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Effect of analyte properties on the kinetic performance of liquid chromatographic separations

André de Villiers, Frederic Lynen UGent and Patrick Sandra UGent (2009) JOURNAL OF CHROMATOGRAPHY A. 1216(16). p.3431-3442
abstract
Advances in modern high-performance liquid chromatography (HPLC) have led to increased interest in the comparison of the ultimate performance limits of methodologies aimed at increasing the resolving power per unit time. Kinetic plot-based methods have proven invaluable in facilitating such evaluations. However, in bridging the gap between fundamental comparisons and the eventual practical applicability of kinetic performance data, the effect of analyte properties have thus far largely been neglected. Using pharmaceutical compounds as representative real-life analytes, it is demonstrated that noteworthy differences in the optimal kinetic performance of a chromatographic system are observed compared to data for common test compounds. For a given stationary phase particle size, higher optimal- and maximum plate numbers, corresponding to increased analysis times, are measured for pharmaceutical compounds. Moreover, it is found that the optimal particle size/maximum pressure combination depends on the analyte under investigation, with the beneficial range of efficiencies for small particles shifted towards higher plate numbers for drug molecules. It is further demonstrated that the pH of the mobile phase plays a crucial role in determining the kinetic performance of pharmaceutical compounds. These data clearly indicate that data for test compounds do not reflect the performance attainable for pharmaceutical compounds and highlights the importance of using real-life samples to perform kinetic evaluations.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
journal title
JOURNAL OF CHROMATOGRAPHY A
J. Chromatogr. A
volume
1216
issue
16
pages
3431 - 3442
conference name
32nd International Symposium on High Performance Liquid Phase Separations and Related Techniques
conference location
Baltimore, MD (USA)
conference start
2008-05-10
conference end
2008-05-16
Web of Science type
Proceedings Paper
Web of Science id
000265262300003
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
4.101 (2009)
JCR rank
4/70 (2009)
JCR quartile
1 (2009)
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.11.101
language
English
UGent publication?
yes
classification
A1
id
668363
handle
http://hdl.handle.net/1854/LU-668363
date created
2009-05-27 11:21:35
date last changed
2009-05-27 11:26:48
@article{668363,
  abstract     = {Advances in modern high-performance liquid chromatography (HPLC) have led to increased interest in the comparison of the ultimate performance limits of methodologies aimed at increasing the resolving power per unit time. Kinetic plot-based methods have proven invaluable in facilitating such evaluations. However, in bridging the gap between fundamental comparisons and the eventual practical applicability of kinetic performance data, the effect of analyte properties have thus far largely been neglected. Using pharmaceutical compounds as representative real-life analytes, it is demonstrated that noteworthy differences in the optimal kinetic performance of a chromatographic system are observed compared to data for common test compounds. For a given stationary phase particle size, higher optimal- and maximum plate numbers, corresponding to increased analysis times, are measured for pharmaceutical compounds. Moreover, it is found that the optimal particle size/maximum pressure combination depends on the analyte under investigation, with the beneficial range of efficiencies for small particles shifted towards higher plate numbers for drug molecules. It is further demonstrated that the pH of the mobile phase plays a crucial role in determining the kinetic performance of pharmaceutical compounds. These data clearly indicate that data for test compounds do not reflect the performance attainable for pharmaceutical compounds and highlights the importance of using real-life samples to perform kinetic evaluations.},
  author       = {de Villiers, Andr{\'e} and Lynen, Frederic and Sandra, Patrick},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  location     = {Baltimore, MD (USA)},
  number       = {16},
  pages        = {3431--3442},
  title        = {Effect of analyte properties on the kinetic performance of liquid chromatographic separations},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.11.101},
  volume       = {1216},
  year         = {2009},
}

Chicago
de Villiers, André, Frederic Lynen, and Patrick Sandra. 2009. “Effect of Analyte Properties on the Kinetic Performance of Liquid Chromatographic Separations.” Journal of Chromatography A 1216 (16): 3431–3442.
APA
de Villiers, André, Lynen, F., & Sandra, P. (2009). Effect of analyte properties on the kinetic performance of liquid chromatographic separations. JOURNAL OF CHROMATOGRAPHY A, 1216(16), 3431–3442. Presented at the 32nd International Symposium on High Performance Liquid Phase Separations and Related Techniques.
Vancouver
1.
de Villiers A, Lynen F, Sandra P. Effect of analyte properties on the kinetic performance of liquid chromatographic separations. JOURNAL OF CHROMATOGRAPHY A. 2009;1216(16):3431–42.
MLA
de Villiers, André, Frederic Lynen, and Patrick Sandra. “Effect of Analyte Properties on the Kinetic Performance of Liquid Chromatographic Separations.” JOURNAL OF CHROMATOGRAPHY A 1216.16 (2009): 3431–3442. Print.