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Multiple Step Gradient Analysis in Stationary Phase Optimised Selectivity LC for the Analysis of Complex Mixtures

Maarten De Beer UGent, Frederic Lynen UGent, Melissa-Hanna Brown and Patrick Sandra UGent (2009) CHROMATOGRAPHIA. 69(7-8). p.609-614
abstract
Stationary phase optimised selectivity liquid chromatography (SOSLC) is an approach to tune a given LC separation by combining different stationary phases in a multi- segment column set-up. The presently available SOSLC optimisation procedure and algorithm are, however, only applicable to isocratic conditions. This is a severe limitation for the analysis of mixtures composed of components covering a broad hydrophobicity range. A strategy is described to circumvent this limitation. The components of a mixture are divided into different groups according to hydrophobicity as elucidated by a gradient analysis on a C-18 reversed-phase column. Each group separation is then individually optimised with a specific isocratic mobile phase composition using the original SOSLC strategy. The mobile phase composition thereby only differs in the percentage of organic modifier between the various groups. Finally, a combination of stationary phases that guarantees sufficient selectivity for all the groups is selected and the separation is performed by a multiple step gradient, whereby each level consists of the mobile phase composition applied for the SOSLC optimisation of the individual groups. The multi step gradient approach is demonstrated through the analysis of a mixture of 27 steroids covering a wide range of hydrophobicity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
CHROMATOGRAPHIA
volume
69
issue
7-8
pages
609 - 614
Web of Science type
Article
Web of Science id
000264885400003
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
1.098 (2009)
JCR rank
48/70 (2009)
JCR quartile
3 (2009)
ISSN
0009-5893
DOI
10.1365/s10337-008-0942-6
language
English
UGent publication?
yes
classification
A1
id
668313
handle
http://hdl.handle.net/1854/LU-668313
date created
2009-05-27 11:15:49
date last changed
2009-05-27 11:20:28
@article{668313,
  abstract     = {Stationary phase optimised selectivity liquid chromatography (SOSLC) is an approach to tune a given LC separation by combining different stationary phases in a multi- segment column set-up. The presently available SOSLC optimisation procedure and algorithm are, however, only applicable to isocratic conditions. This is a severe limitation for the analysis of mixtures composed of components covering a broad hydrophobicity range. A strategy is described to circumvent this limitation. The components of a mixture are divided into different groups according to hydrophobicity as elucidated by a gradient analysis on a C-18 reversed-phase column. Each group separation is then individually optimised with a specific isocratic mobile phase composition using the original SOSLC strategy. The mobile phase composition thereby only differs in the percentage of organic modifier between the various groups. Finally, a combination of stationary phases that guarantees sufficient selectivity for all the groups is selected and the separation is performed by a multiple step gradient, whereby each level consists of the mobile phase composition applied for the SOSLC optimisation of the individual groups. The multi step gradient approach is demonstrated through the analysis of a mixture of 27 steroids covering a wide range of hydrophobicity.},
  author       = {De Beer, Maarten and Lynen, Frederic and Brown, Melissa-Hanna and Sandra, Patrick},
  issn         = {0009-5893},
  journal      = {CHROMATOGRAPHIA},
  language     = {eng},
  number       = {7-8},
  pages        = {609--614},
  title        = {Multiple Step Gradient Analysis in Stationary Phase Optimised Selectivity LC for the Analysis of Complex Mixtures},
  url          = {http://dx.doi.org/10.1365/s10337-008-0942-6},
  volume       = {69},
  year         = {2009},
}

Chicago
De Beer, Maarten, Frederic Lynen, Melissa-Hanna Brown, and Patrick Sandra. 2009. “Multiple Step Gradient Analysis in Stationary Phase Optimised Selectivity LC for the Analysis of Complex Mixtures.” Chromatographia 69 (7-8): 609–614.
APA
De Beer, M., Lynen, F., Brown, M.-H., & Sandra, P. (2009). Multiple Step Gradient Analysis in Stationary Phase Optimised Selectivity LC for the Analysis of Complex Mixtures. CHROMATOGRAPHIA, 69(7-8), 609–614.
Vancouver
1.
De Beer M, Lynen F, Brown M-H, Sandra P. Multiple Step Gradient Analysis in Stationary Phase Optimised Selectivity LC for the Analysis of Complex Mixtures. CHROMATOGRAPHIA. 2009;69(7-8):609–14.
MLA
De Beer, Maarten, Frederic Lynen, Melissa-Hanna Brown, et al. “Multiple Step Gradient Analysis in Stationary Phase Optimised Selectivity LC for the Analysis of Complex Mixtures.” CHROMATOGRAPHIA 69.7-8 (2009): 609–614. Print.