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Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH's

Isabelle François UGent, Deirdre Cabooter, Koen Sandra, Frederic Lynen UGent, Gert Desmet and Patrick Sandra UGent (2009) JOURNAL OF SEPARATION SCIENCE. 32(8). p.1137-1144
abstract
As all alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LC x RP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
32
issue
8
pages
1137 - 1144
Web of Science type
Article
Web of Science id
000265793700005
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.551 (2009)
JCR rank
22/70 (2009)
JCR quartile
2 (2009)
ISSN
1615-9306
DOI
10.1002/jssc.200800578
language
English
UGent publication?
yes
classification
A1
id
668251
handle
http://hdl.handle.net/1854/LU-668251
date created
2009-05-27 11:11:14
date last changed
2009-05-27 11:14:53
@article{668251,
  abstract     = {As all alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LC x RP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.},
  author       = {Fran\c{c}ois, Isabelle and Cabooter, Deirdre and Sandra, Koen and Lynen, Frederic and Desmet, Gert and Sandra, Patrick},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  language     = {eng},
  number       = {8},
  pages        = {1137--1144},
  title        = {Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH's},
  url          = {http://dx.doi.org/10.1002/jssc.200800578},
  volume       = {32},
  year         = {2009},
}

Chicago
François, Isabelle, Deirdre Cabooter, Koen Sandra, Frederic Lynen, Gert Desmet, and Patrick Sandra. 2009. “Tryptic Digest Analysis by Comprehensive Reversed Phase x Two Reversed Phase Liquid Chromatography (RP-LC x 2RP-LC) at Different pH’s.” Journal of Separation Science 32 (8): 1137–1144.
APA
François, I., Cabooter, D., Sandra, K., Lynen, F., Desmet, G., & Sandra, P. (2009). Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH’s. JOURNAL OF SEPARATION SCIENCE, 32(8), 1137–1144.
Vancouver
1.
François I, Cabooter D, Sandra K, Lynen F, Desmet G, Sandra P. Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH’s. JOURNAL OF SEPARATION SCIENCE. 2009;32(8):1137–44.
MLA
François, Isabelle, Deirdre Cabooter, Koen Sandra, et al. “Tryptic Digest Analysis by Comprehensive Reversed Phase x Two Reversed Phase Liquid Chromatography (RP-LC x 2RP-LC) at Different pH’s.” JOURNAL OF SEPARATION SCIENCE 32.8 (2009): 1137–1144. Print.