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Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques

Koen Sandra, Mahan Moshir, Filip D'hondt, Robin Tuytten, Katleen Verleysen, Koen Kas UGent, Isabelle François UGent and Patrick Sandra UGent (2009) JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 877(11-12). p.1019-1039
abstract
Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
journal title
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
J. Chromatogr. B
volume
877
issue
11-12
pages
1019 - 1039
Web of Science type
Review
Web of Science id
000265446400001
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.777 (2009)
JCR rank
14/70 (2009)
JCR quartile
1 (2009)
ISSN
1570-0232
DOI
10.1016/j.jchromb.2009.02.050
language
English
UGent publication?
yes
classification
A1
id
668065
handle
http://hdl.handle.net/1854/LU-668065
date created
2009-05-27 10:43:52
date last changed
2016-12-19 15:43:47
@article{668065,
  abstract     = {Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.},
  author       = {Sandra, Koen and Moshir, Mahan and D'hondt, Filip and Tuytten, Robin and Verleysen, Katleen and Kas, Koen and Fran\c{c}ois, Isabelle and Sandra, Patrick},
  issn         = {1570-0232},
  journal      = {JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES},
  language     = {eng},
  number       = {11-12},
  pages        = {1019--1039},
  title        = {Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2009.02.050},
  volume       = {877},
  year         = {2009},
}

Chicago
Sandra, Koen, Mahan Moshir, Filip D’hondt, Robin Tuytten, Katleen Verleysen, Koen Kas, Isabelle François, and Patrick Sandra. 2009. “Highly Efficient Peptide Separations in Proteomics Part 2: Bi- and Multidimensional Liquid-based Separation Techniques.” Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences 877 (11-12): 1019–1039.
APA
Sandra, Koen, Moshir, M., D’hondt, F., Tuytten, R., Verleysen, K., Kas, K., François, I., et al. (2009). Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 877(11-12), 1019–1039.
Vancouver
1.
Sandra K, Moshir M, D’hondt F, Tuytten R, Verleysen K, Kas K, et al. Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2009;877(11-12):1019–39.
MLA
Sandra, Koen, Mahan Moshir, Filip D’hondt, et al. “Highly Efficient Peptide Separations in Proteomics Part 2: Bi- and Multidimensional Liquid-based Separation Techniques.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.11-12 (2009): 1019–1039. Print.