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Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques

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Abstract
Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.

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Chicago
Sandra, Koen, Mahan Moshir, Filip D’hondt, Robin Tuytten, Katleen Verleysen, Koen Kas, Isabelle François, and Patrick Sandra. 2009. “Highly Efficient Peptide Separations in Proteomics Part 2: Bi- and Multidimensional Liquid-based Separation Techniques.” Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences 877 (11-12): 1019–1039.
APA
Sandra, Koen, Moshir, M., D’hondt, F., Tuytten, R., Verleysen, K., Kas, K., François, I., et al. (2009). Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 877(11-12), 1019–1039.
Vancouver
1.
Sandra K, Moshir M, D’hondt F, Tuytten R, Verleysen K, Kas K, et al. Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2009;877(11-12):1019–39.
MLA
Sandra, Koen, Mahan Moshir, Filip D’hondt, et al. “Highly Efficient Peptide Separations in Proteomics Part 2: Bi- and Multidimensional Liquid-based Separation Techniques.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.11-12 (2009): 1019–1039. Print.
@article{668065,
  abstract     = {Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.},
  author       = {Sandra, Koen and Moshir, Mahan and D'hondt, Filip and Tuytten, Robin and Verleysen, Katleen and Kas, Koen and Fran\c{c}ois, Isabelle and Sandra, Patrick},
  issn         = {1570-0232},
  journal      = {JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES},
  language     = {eng},
  number       = {11-12},
  pages        = {1019--1039},
  title        = {Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2009.02.050},
  volume       = {877},
  year         = {2009},
}

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