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Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica

(2008) JOURNAL OF CHROMATOGRAPHY A. 1185(2). p.263-272
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Abstract
A method has been developed for the analysis of a broad spectrum of pharmaceuticals using packed column supercritical fluid chromatography (pSFC) on a cyanopropyl silicagel stationary phase. Five 25 cm x 4.6 mm 1.D., 5.0 mu m columns were coupled to generate ca. 100 000 plates. The selectivity was tuned by varying the nature and concentration of various modifiers and additives in the carbon dioxide mobile phase. It was noted that pressure influences both efficiency and selectivity of the chromatographic process. Final method conditions are: outlet pressure 100 bar, flow 2.0 mL/min, temperature 40 degrees C, organic modifier program from 5% (1 min) to 40% at 2.0%/min, organic modifier composition methanol: acetonitrile in a ratio of 3:1 (variable according to sample composition) with peak symmetry additives trifluoroacetic acid and diisopropylamine both at levels of 0.5%.

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Citation

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MLA
Brunelli, Claudio et al. “Development of a Supercritical Fluid Chromatography High-resolution Separation Method Suitable for Pharmaceuticals Using Cyanopropyl Silica.” JOURNAL OF CHROMATOGRAPHY A 1185.2 (2008): 263–272. Print.
APA
Brunelli, C., Zhao, Y., Brown, M.-H., & Sandra, P. (2008). Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica. JOURNAL OF CHROMATOGRAPHY A, 1185(2), 263–272.
Chicago author-date
Brunelli, Claudio, Yining Zhao, Melissa-Hanna Brown, and Patrick Sandra. 2008. “Development of a Supercritical Fluid Chromatography High-resolution Separation Method Suitable for Pharmaceuticals Using Cyanopropyl Silica.” Journal of Chromatography A 1185 (2): 263–272.
Chicago author-date (all authors)
Brunelli, Claudio, Yining Zhao, Melissa-Hanna Brown, and Patrick Sandra. 2008. “Development of a Supercritical Fluid Chromatography High-resolution Separation Method Suitable for Pharmaceuticals Using Cyanopropyl Silica.” Journal of Chromatography A 1185 (2): 263–272.
Vancouver
1.
Brunelli C, Zhao Y, Brown M-H, Sandra P. Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica. JOURNAL OF CHROMATOGRAPHY A. 2008;1185(2):263–72.
IEEE
[1]
C. Brunelli, Y. Zhao, M.-H. Brown, and P. Sandra, “Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica,” JOURNAL OF CHROMATOGRAPHY A, vol. 1185, no. 2, pp. 263–272, 2008.
@article{668012,
  abstract     = {A method has been developed for the analysis of a broad spectrum of pharmaceuticals using packed column supercritical fluid chromatography (pSFC) on a cyanopropyl silicagel stationary phase. Five 25 cm x 4.6 mm 1.D., 5.0 mu m columns were coupled to generate ca. 100 000 plates. The selectivity was tuned by varying the nature and concentration of various modifiers and additives in the carbon dioxide mobile phase. It was noted that pressure influences both efficiency and selectivity of the chromatographic process. Final method conditions are: outlet pressure 100 bar, flow 2.0 mL/min, temperature 40 degrees C, organic modifier program from 5% (1 min) to 40% at 2.0%/min, organic modifier composition methanol: acetonitrile in a ratio of 3:1 (variable according to sample composition) with peak symmetry additives trifluoroacetic acid and diisopropylamine both at levels of 0.5%.},
  author       = {Brunelli, Claudio and Zhao, Yining and Brown, Melissa-Hanna and Sandra, Patrick},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  number       = {2},
  pages        = {263--272},
  title        = {Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.01.050},
  volume       = {1185},
  year         = {2008},
}

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