Ghent University Academic Bibliography

Advanced

Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica

Claudio Brunelli, Yining Zhao, Melissa-Hanna Brown and Patrick Sandra UGent (2008) JOURNAL OF CHROMATOGRAPHY A. 1185(2). p.263-272
abstract
A method has been developed for the analysis of a broad spectrum of pharmaceuticals using packed column supercritical fluid chromatography (pSFC) on a cyanopropyl silicagel stationary phase. Five 25 cm x 4.6 mm 1.D., 5.0 mu m columns were coupled to generate ca. 100 000 plates. The selectivity was tuned by varying the nature and concentration of various modifiers and additives in the carbon dioxide mobile phase. It was noted that pressure influences both efficiency and selectivity of the chromatographic process. Final method conditions are: outlet pressure 100 bar, flow 2.0 mL/min, temperature 40 degrees C, organic modifier program from 5% (1 min) to 40% at 2.0%/min, organic modifier composition methanol: acetonitrile in a ratio of 3:1 (variable according to sample composition) with peak symmetry additives trifluoroacetic acid and diisopropylamine both at levels of 0.5%.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
JOURNAL OF CHROMATOGRAPHY A
J. Chromatogr. A
volume
1185
issue
2
pages
263 - 272
Web of Science type
Article
Web of Science id
000254494900014
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.756 (2008)
JCR rank
6/70 (2008)
JCR quartile
1 (2008)
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.01.050
language
English
UGent publication?
yes
classification
A1
id
668012
handle
http://hdl.handle.net/1854/LU-668012
date created
2009-05-27 10:38:32
date last changed
2009-05-27 10:43:00
@article{668012,
  abstract     = {A method has been developed for the analysis of a broad spectrum of pharmaceuticals using packed column supercritical fluid chromatography (pSFC) on a cyanopropyl silicagel stationary phase. Five 25 cm x 4.6 mm 1.D., 5.0 mu m columns were coupled to generate ca. 100 000 plates. The selectivity was tuned by varying the nature and concentration of various modifiers and additives in the carbon dioxide mobile phase. It was noted that pressure influences both efficiency and selectivity of the chromatographic process. Final method conditions are: outlet pressure 100 bar, flow 2.0 mL/min, temperature 40 degrees C, organic modifier program from 5\% (1 min) to 40\% at 2.0\%/min, organic modifier composition methanol: acetonitrile in a ratio of 3:1 (variable according to sample composition) with peak symmetry additives trifluoroacetic acid and diisopropylamine both at levels of 0.5\%.},
  author       = {Brunelli, Claudio and Zhao, Yining and Brown, Melissa-Hanna and Sandra, Patrick},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  number       = {2},
  pages        = {263--272},
  title        = {Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.01.050},
  volume       = {1185},
  year         = {2008},
}

Chicago
Brunelli, Claudio, Yining Zhao, Melissa-Hanna Brown, and Patrick Sandra. 2008. “Development of a Supercritical Fluid Chromatography High-resolution Separation Method Suitable for Pharmaceuticals Using Cyanopropyl Silica.” Journal of Chromatography A 1185 (2): 263–272.
APA
Brunelli, C., Zhao, Y., Brown, M.-H., & Sandra, P. (2008). Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica. JOURNAL OF CHROMATOGRAPHY A, 1185(2), 263–272.
Vancouver
1.
Brunelli C, Zhao Y, Brown M-H, Sandra P. Development of a supercritical fluid chromatography high-resolution separation method suitable for pharmaceuticals using cyanopropyl silica. JOURNAL OF CHROMATOGRAPHY A. 2008;1185(2):263–72.
MLA
Brunelli, Claudio, Yining Zhao, Melissa-Hanna Brown, et al. “Development of a Supercritical Fluid Chromatography High-resolution Separation Method Suitable for Pharmaceuticals Using Cyanopropyl Silica.” JOURNAL OF CHROMATOGRAPHY A 1185.2 (2008): 263–272. Print.