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Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphtalene derivatives for amperometric determination of cholesterol

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Abstract
Cholesterol amperometric biosensors constructed with enzymes entrapped in electropolymerized layers of polypyrrole and poly-naphthalene derivative polymers are compared. The biosensors are based on entrapment of cholesterol oxidase and/or cholesterol esterase in monolayer or multilayer films electrochemically synthesised from pyrrole, 1,8-diaminonaphthalene (1,8-DAN), and 1,5-diaminonaphthalene (1,5-DAN) monomers. Seven configurations were assayed and compared, and different analytical properties were obtained depending on the kind of polymer and the arrangement of the layers. The selectivity properties were evaluated for the different monolayer and bilayer configurations proposed as a function of the film permeation factor. All the steps involved in the preparation of the biosensors and determination of cholesterol were carried out in a flow system. Sensitivity and selectivity depend greatly on hydrophobicity, permeability, compactness, thickness, and the kind of the polymer used. In some cases a protective outer layer of non-conducting poly(o-phenylenediamine) polymer improves the analytical characteristics of the biosensor. A comparative study was made of the analytical performance of each of the configurations developed. The biosensors were also applied to the flow-injection determination of cholesterol in a synthetic serum.

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Citation

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Chicago
Vidal, Juan Carlos, Esperanza Garcia-Ruiz, Javier Espuelas, Maria Aramendia, and Juan Ramon Castillo. 2003. “Comparison of Biosensors Based on Entrapment of Cholesterol Oxidase and Cholesterol Esterase in Electropolymerized Films of Polypyrrole and Diaminonaphtalene Derivatives for Amperometric Determination of Cholesterol.” Analytical and Bioanalytical Chemistry 377 (2): 273–280.
APA
Vidal, J. C., Garcia-Ruiz, E., Espuelas, J., Aramendia, M., & Castillo, J. R. (2003). Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphtalene derivatives for amperometric determination of cholesterol. Analytical and Bioanalytical Chemistry, 377(2), 273–280.
Vancouver
1.
Vidal JC, Garcia-Ruiz E, Espuelas J, Aramendia M, Castillo JR. Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphtalene derivatives for amperometric determination of cholesterol. Analytical and Bioanalytical Chemistry. 2003;377(2):273–80.
MLA
Vidal, Juan Carlos, Esperanza Garcia-Ruiz, Javier Espuelas, et al. “Comparison of Biosensors Based on Entrapment of Cholesterol Oxidase and Cholesterol Esterase in Electropolymerized Films of Polypyrrole and Diaminonaphtalene Derivatives for Amperometric Determination of Cholesterol.” Analytical and Bioanalytical Chemistry 377.2 (2003): 273–280. Print.
@article{667906,
  abstract     = {Cholesterol amperometric biosensors constructed with enzymes entrapped in electropolymerized layers of polypyrrole and poly-naphthalene derivative polymers are compared. The biosensors are based on entrapment of cholesterol oxidase and/or cholesterol esterase in monolayer or multilayer films electrochemically synthesised from pyrrole, 1,8-diaminonaphthalene (1,8-DAN), and 1,5-diaminonaphthalene (1,5-DAN) monomers. Seven configurations were assayed and compared, and different analytical properties were obtained depending on the kind of polymer and the arrangement of the layers. The selectivity properties were evaluated for the different monolayer and bilayer configurations proposed as a function of the film permeation factor. All the steps involved in the preparation of the biosensors and determination of cholesterol were carried out in a flow system. Sensitivity and selectivity depend greatly on hydrophobicity, permeability, compactness, thickness, and the kind of the polymer used. In some cases a protective outer layer of non-conducting poly(o-phenylenediamine) polymer improves the analytical characteristics of the biosensor. A comparative study was made of the analytical performance of each of the configurations developed. The biosensors were also applied to the flow-injection determination of cholesterol in a synthetic serum.},
  author       = {Vidal, Juan Carlos and Garcia-Ruiz, Esperanza and Espuelas, Javier and Aramendia, Maria and Castillo, Juan Ramon},
  issn         = {1618-2642},
  journal      = {Analytical and Bioanalytical Chemistry},
  language     = {eng},
  number       = {2},
  pages        = {273--280},
  title        = {Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphtalene derivatives for amperometric determination of cholesterol},
  url          = {http://dx.doi.org/10.1007/s00216-003-2120-x},
  volume       = {377},
  year         = {2003},
}

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