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Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2-ethylpyridine column

Claudio Brunelli, Yining Zha, Melissa-Hanna Brown and Patrick Sandra UGent (2008) JOURNAL OF SEPARATION SCIENCE. 31(8). p.1299-1306
abstract
The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2-ethylpyridine column (25 cm x 4.6 mm id, 3 mu m particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open-access generic screening environments.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
31
issue
8
pages
1299 - 1306
Web of Science type
Article
Web of Science id
000256160000009
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.746 (2008)
JCR rank
17/70 (2008)
JCR quartile
1 (2008)
ISSN
1615-9306
DOI
10.1002/jssc.200700555
language
English
UGent publication?
yes
classification
A1
id
667867
handle
http://hdl.handle.net/1854/LU-667867
date created
2009-05-27 10:09:03
date last changed
2009-05-27 10:13:24
@article{667867,
  abstract     = {The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2-ethylpyridine column (25 cm x 4.6 mm id, 3 mu m particles) is presented. The optimization strategy involved separations at 100\% methanol (MeOH) and at 50\% MeOH/50\% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25\% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65\% MeOH/35\% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open-access generic screening environments.},
  author       = {Brunelli, Claudio and Zha, Yining and Brown, Melissa-Hanna and Sandra, Patrick},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  language     = {eng},
  number       = {8},
  pages        = {1299--1306},
  title        = {Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2-ethylpyridine column},
  url          = {http://dx.doi.org/10.1002/jssc.200700555},
  volume       = {31},
  year         = {2008},
}

Chicago
Brunelli, Claudio, Yining Zha, Melissa-Hanna Brown, and Patrick Sandra. 2008. “Pharmaceutical Analysis by Supercritical Fluid Chromatography: Optimization of the Mobile Phase Composition on a 2-ethylpyridine Column.” Journal of Separation Science 31 (8): 1299–1306.
APA
Brunelli, C., Zha, Y., Brown, M.-H., & Sandra, P. (2008). Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2-ethylpyridine column. JOURNAL OF SEPARATION SCIENCE, 31(8), 1299–1306.
Vancouver
1.
Brunelli C, Zha Y, Brown M-H, Sandra P. Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2-ethylpyridine column. JOURNAL OF SEPARATION SCIENCE. 2008;31(8):1299–306.
MLA
Brunelli, Claudio, Yining Zha, Melissa-Hanna Brown, et al. “Pharmaceutical Analysis by Supercritical Fluid Chromatography: Optimization of the Mobile Phase Composition on a 2-ethylpyridine Column.” JOURNAL OF SEPARATION SCIENCE 31.8 (2008): 1299–1306. Print.