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Serial coupling of reversed-phase and hydrophilic interaction liquid chromatography to broaden the elution window for the analysis of pharmaceutical compounds

Stefan Louw UGent, Alberto Dos Santos Pereira UGent, Frederic Lynen UGent, Melissa Hanna-Brown and Patrick Sandra UGent (2008) JOURNAL OF CHROMATOGRAPHY A. 1208(1-2). p.90-94
abstract
It is presently a common practice in drug discovery to analyse samples by reversed-phase liquid chromatography (RPLC) and hydrophilic interaction chromatography (HILIC). To increase throughput, HILIC was connected in series to RPLC by means of a T-piece with make-up flow. The first column is a 2 mm I.D. column having an optimal flow between 0.1 and 0.2 mL/min. Via the T-piece. the flow for the second dimension column with an I.D. of 4.6 mm is adjusted to 1.5-2.0 mL/min with a high acetonitrile content (i.e. >= 80%) mobile phase. Therefore, even in gradient RPLC analysis starting with a mobile phase with high water content, the HILIC column is always operated at high acetonitrile concentration which is required to obtain retention on the HILIC column. The performance of the hyphenated RPLC/HILIC set-up is illustrated with the analysis of two model samples of pharmaceutical interest. Optimization of the conditions in the HILIC dimension is discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
JOURNAL OF CHROMATOGRAPHY A
J. Chromatogr. A
volume
1208
issue
1-2
pages
90 - 94
Web of Science type
Article
Web of Science id
000260445900013
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.756 (2008)
JCR rank
6/70 (2008)
JCR quartile
1 (2008)
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.08.058
language
English
UGent publication?
yes
classification
A1
id
667818
handle
http://hdl.handle.net/1854/LU-667818
date created
2009-05-27 09:55:22
date last changed
2009-05-27 10:00:34
@article{667818,
  abstract     = {It is presently a common practice in drug discovery to analyse samples by reversed-phase liquid chromatography (RPLC) and hydrophilic interaction chromatography (HILIC). To increase throughput, HILIC was connected in series to RPLC by means of a T-piece with make-up flow. The first column is a 2 mm I.D. column having an optimal flow between 0.1 and 0.2 mL/min. Via the T-piece. the flow for the second dimension column with an I.D. of 4.6 mm is adjusted to 1.5-2.0 mL/min with a high acetonitrile content (i.e. {\textrangle}= 80\%) mobile phase. Therefore, even in gradient RPLC analysis starting with a mobile phase with high water content, the HILIC column is always operated at high acetonitrile concentration which is required to obtain retention on the HILIC column. The performance of the hyphenated RPLC/HILIC set-up is illustrated with the analysis of two model samples of pharmaceutical interest. Optimization of the conditions in the HILIC dimension is discussed.},
  author       = {Louw, Stefan and Dos Santos Pereira, Alberto and Lynen, Frederic and Hanna-Brown, Melissa and Sandra, Patrick},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  number       = {1-2},
  pages        = {90--94},
  title        = {Serial coupling of reversed-phase and hydrophilic interaction liquid chromatography to broaden the elution window for the analysis of pharmaceutical compounds},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.08.058},
  volume       = {1208},
  year         = {2008},
}

Chicago
Louw, Stefan, Alberto Dos Santos Pereira, Frederic Lynen, Melissa Hanna-Brown, and Patrick Sandra. 2008. “Serial Coupling of Reversed-phase and Hydrophilic Interaction Liquid Chromatography to Broaden the Elution Window for the Analysis of Pharmaceutical Compounds.” Journal of Chromatography A 1208 (1-2): 90–94.
APA
Louw, S., Dos Santos Pereira, A., Lynen, F., Hanna-Brown, M., & Sandra, P. (2008). Serial coupling of reversed-phase and hydrophilic interaction liquid chromatography to broaden the elution window for the analysis of pharmaceutical compounds. JOURNAL OF CHROMATOGRAPHY A, 1208(1-2), 90–94.
Vancouver
1.
Louw S, Dos Santos Pereira A, Lynen F, Hanna-Brown M, Sandra P. Serial coupling of reversed-phase and hydrophilic interaction liquid chromatography to broaden the elution window for the analysis of pharmaceutical compounds. JOURNAL OF CHROMATOGRAPHY A. 2008;1208(1-2):90–4.
MLA
Louw, Stefan, Alberto Dos Santos Pereira, Frederic Lynen, et al. “Serial Coupling of Reversed-phase and Hydrophilic Interaction Liquid Chromatography to Broaden the Elution Window for the Analysis of Pharmaceutical Compounds.” JOURNAL OF CHROMATOGRAPHY A 1208.1-2 (2008): 90–94. Print.