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Evaluation of a molecularly imprinted polymer as in-line concentrator in capillary electrophoresis

Francisco J. Lara, Frederic Lynen UGent, Patrick Sandra UGent, Ana M. Garcia-Campana and Fermin Ales-Barrero (2008) ELECTROPHORESIS. 29(18). p.3834-3841
abstract
Molecularly imprinted polymers (MIPs) have been evaluated as sorbent for the construction of an in-line solid phase extraction concentrator in capillary electrophoresis to be applied in the monitoring of triazine herbicides: atrazine and its three metabolites, desethylatrazine, desisopropylatrazine and desethyldesisopropylatrazine. Initially, the electrophoretic separation of these compounds was optimized. The electrolyte consists of an aqueous solution of 75 mM phosphoric acid (H3PO4) adjusted to pH 2.1 and containing 0.7 mM cetyltrimethylammonium bromide. After the fabrication and assembly of the concentrator into the capillary, these optimal CE conditions were applied to evaluate the performance of this device. Efficiencies of 40 000-55 000 plates could be achieved and the separation time was around one hour. Different parameters affecting the in-line molecularly imprinted solid-phase extraction in capillary electrophoresis such as composition and volume of the elution plug were optimized. The method was evaluated in terms of linearity, precision and limits of detection and quantification. MIPs were compared with Oasis hydrophilic-lipophilic-balance (HLB) particles for the in-line coupling of solid-phase extraction and capillary electrophoresis. The superior selectivity of MIPs is demonstrated through direct injection of a urine sample spiked with 10 mu g/mL atrazine, desethylatrazine, desisopropylatrazine and desethyldesisopropylatrazine. Recoveries were between 92 and 102% compared with an aqueous solution.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
ELECTROPHORESIS
volume
29
issue
18
pages
3834 - 3841
Web of Science type
Article
Web of Science id
000260242100016
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.509 (2008)
JCR rank
7/70 (2008)
JCR quartile
1 (2008)
ISSN
0173-0835
DOI
10.1002/elps.200700889
language
English
UGent publication?
yes
classification
A1
id
667781
handle
http://hdl.handle.net/1854/LU-667781
date created
2009-05-27 09:49:34
date last changed
2009-05-27 09:54:37
@article{667781,
  abstract     = {Molecularly imprinted polymers (MIPs) have been evaluated as sorbent for the construction of an in-line solid phase extraction concentrator in capillary electrophoresis to be applied in the monitoring of triazine herbicides: atrazine and its three metabolites, desethylatrazine, desisopropylatrazine and desethyldesisopropylatrazine. Initially, the electrophoretic separation of these compounds was optimized. The electrolyte consists of an aqueous solution of 75 mM phosphoric acid (H3PO4) adjusted to pH 2.1 and containing 0.7 mM cetyltrimethylammonium bromide. After the fabrication and assembly of the concentrator into the capillary, these optimal CE conditions were applied to evaluate the performance of this device. Efficiencies of 40 000-55 000 plates could be achieved and the separation time was around one hour. Different parameters affecting the in-line molecularly imprinted solid-phase extraction in capillary electrophoresis such as composition and volume of the elution plug were optimized. The method was evaluated in terms of linearity, precision and limits of detection and quantification. MIPs were compared with Oasis hydrophilic-lipophilic-balance (HLB) particles for the in-line coupling of solid-phase extraction and capillary electrophoresis. The superior selectivity of MIPs is demonstrated through direct injection of a urine sample spiked with 10 mu g/mL atrazine, desethylatrazine, desisopropylatrazine and desethyldesisopropylatrazine. Recoveries were between 92 and 102\% compared with an aqueous solution.},
  author       = {Lara, Francisco J. and Lynen, Frederic and Sandra, Patrick and Garcia-Campana, Ana M. and Ales-Barrero, Fermin},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  language     = {eng},
  number       = {18},
  pages        = {3834--3841},
  title        = {Evaluation of a molecularly imprinted polymer as in-line concentrator in capillary electrophoresis},
  url          = {http://dx.doi.org/10.1002/elps.200700889},
  volume       = {29},
  year         = {2008},
}

Chicago
Lara, Francisco J., Frederic Lynen, Patrick Sandra, Ana M. Garcia-Campana, and Fermin Ales-Barrero. 2008. “Evaluation of a Molecularly Imprinted Polymer as In-line Concentrator in Capillary Electrophoresis.” Electrophoresis 29 (18): 3834–3841.
APA
Lara, F. J., Lynen, F., Sandra, P., Garcia-Campana, A. M., & Ales-Barrero, F. (2008). Evaluation of a molecularly imprinted polymer as in-line concentrator in capillary electrophoresis. ELECTROPHORESIS, 29(18), 3834–3841.
Vancouver
1.
Lara FJ, Lynen F, Sandra P, Garcia-Campana AM, Ales-Barrero F. Evaluation of a molecularly imprinted polymer as in-line concentrator in capillary electrophoresis. ELECTROPHORESIS. 2008;29(18):3834–41.
MLA
Lara, Francisco J., Frederic Lynen, Patrick Sandra, et al. “Evaluation of a Molecularly Imprinted Polymer as In-line Concentrator in Capillary Electrophoresis.” ELECTROPHORESIS 29.18 (2008): 3834–3841. Print.