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Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins

Luis Saavedra, Beverly Nickerson, Ricardo E. Borjas, Frederic Lynen UGent and Patrick Sandra UGent (2008) JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 875(1). p.248-253
abstract
A multiplexed capillary electrophoresis (CE) system equipped with 96 channels was evaluated for high-throughput screening of enantiomers of solutes of pharmaceutical interest. Using highly (DS similar to 12) sulphated alpha-, beta- and gamma-cyclodextrins under acidic conditions (pH 2.5) only 48 channels could be used because of the high conductivity of the chiral selectors. method transfer from a single channel to a 48 channel CE system is described. Under optimised conditions, the analysis time on the multiplexed 48 channel CE system is ca. five to eight times the analysis time on the single channel CE system. The figures of merit for the multiplexed system are presented as well as performance evaluation including throughput and productivity gain. Intra-day precision (n = 6) ranged from 2.0 to 16.5% and from 2.2 to 15.5% for migration time and resolution, respectively. These values increased with ca. 10% for intermediate precision.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
J. Chromatogr. B
volume
875
issue
1
pages
248 - 253
Web of Science type
Article
Web of Science id
000260945800033
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.5 (2008)
JCR rank
21/70 (2008)
JCR quartile
2 (2008)
ISSN
1570-0232
DOI
10.1016/j.jchromb.2008.07.020
language
English
UGent publication?
yes
classification
A1
id
667748
handle
http://hdl.handle.net/1854/LU-667748
date created
2009-05-27 09:43:19
date last changed
2009-05-27 09:48:13
@article{667748,
  abstract     = {A multiplexed capillary electrophoresis (CE) system equipped with 96 channels was evaluated for high-throughput screening of enantiomers of solutes of pharmaceutical interest. Using highly (DS similar to 12) sulphated alpha-, beta- and gamma-cyclodextrins under acidic conditions (pH 2.5) only 48 channels could be used because of the high conductivity of the chiral selectors. method transfer from a single channel to a 48 channel CE system is described. Under optimised conditions, the analysis time on the multiplexed 48 channel CE system is ca. five to eight times the analysis time on the single channel CE system. The figures of merit for the multiplexed system are presented as well as performance evaluation including throughput and productivity gain. Intra-day precision (n = 6) ranged from 2.0 to 16.5\% and from 2.2 to 15.5\% for migration time and resolution, respectively. These values increased with ca. 10\% for intermediate precision.},
  author       = {Saavedra, Luis and Nickerson, Beverly and Borjas, Ricardo E. and Lynen, Frederic and Sandra, Patrick},
  issn         = {1570-0232},
  journal      = {JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES},
  language     = {eng},
  number       = {1},
  pages        = {248--253},
  title        = {Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2008.07.020},
  volume       = {875},
  year         = {2008},
}

Chicago
Saavedra, Luis, Beverly Nickerson, Ricardo E. Borjas, Frederic Lynen, and Patrick Sandra. 2008. “Enantioseparation of Pharmaceutical Compounds by Multiplexed Capillary Electrophoresis Using Highly Sulphated Alpha-, Beta- and Gamma-cyclodextrins.” Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences 875 (1): 248–253.
APA
Saavedra, L., Nickerson, B., Borjas, R. E., Lynen, F., & Sandra, P. (2008). Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 875(1), 248–253.
Vancouver
1.
Saavedra L, Nickerson B, Borjas RE, Lynen F, Sandra P. Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2008;875(1):248–53.
MLA
Saavedra, Luis, Beverly Nickerson, Ricardo E. Borjas, et al. “Enantioseparation of Pharmaceutical Compounds by Multiplexed Capillary Electrophoresis Using Highly Sulphated Alpha-, Beta- and Gamma-cyclodextrins.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 875.1 (2008): 248–253. Print.