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Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins

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Abstract
A multiplexed capillary electrophoresis (CE) system equipped with 96 channels was evaluated for high-throughput screening of enantiomers of solutes of pharmaceutical interest. Using highly (DS similar to 12) sulphated alpha-, beta- and gamma-cyclodextrins under acidic conditions (pH 2.5) only 48 channels could be used because of the high conductivity of the chiral selectors. method transfer from a single channel to a 48 channel CE system is described. Under optimised conditions, the analysis time on the multiplexed 48 channel CE system is ca. five to eight times the analysis time on the single channel CE system. The figures of merit for the multiplexed system are presented as well as performance evaluation including throughput and productivity gain. Intra-day precision (n = 6) ranged from 2.0 to 16.5% and from 2.2 to 15.5% for migration time and resolution, respectively. These values increased with ca. 10% for intermediate precision.

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Chicago
Saavedra, Luis, Beverly Nickerson, Ricardo E. Borjas, Frederic Lynen, and Patrick Sandra. 2008. “Enantioseparation of Pharmaceutical Compounds by Multiplexed Capillary Electrophoresis Using Highly Sulphated Alpha-, Beta- and Gamma-cyclodextrins.” Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences 875 (1): 248–253.
APA
Saavedra, L., Nickerson, B., Borjas, R. E., Lynen, F., & Sandra, P. (2008). Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 875(1), 248–253.
Vancouver
1.
Saavedra L, Nickerson B, Borjas RE, Lynen F, Sandra P. Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2008;875(1):248–53.
MLA
Saavedra, Luis et al. “Enantioseparation of Pharmaceutical Compounds by Multiplexed Capillary Electrophoresis Using Highly Sulphated Alpha-, Beta- and Gamma-cyclodextrins.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 875.1 (2008): 248–253. Print.
@article{667748,
  abstract     = {A multiplexed capillary electrophoresis (CE) system equipped with 96 channels was evaluated for high-throughput screening of enantiomers of solutes of pharmaceutical interest. Using highly (DS similar to 12) sulphated alpha-, beta- and gamma-cyclodextrins under acidic conditions (pH 2.5) only 48 channels could be used because of the high conductivity of the chiral selectors. method transfer from a single channel to a 48 channel CE system is described. Under optimised conditions, the analysis time on the multiplexed 48 channel CE system is ca. five to eight times the analysis time on the single channel CE system. The figures of merit for the multiplexed system are presented as well as performance evaluation including throughput and productivity gain. Intra-day precision (n = 6) ranged from 2.0 to 16.5\% and from 2.2 to 15.5\% for migration time and resolution, respectively. These values increased with ca. 10\% for intermediate precision.},
  author       = {Saavedra, Luis and Nickerson, Beverly and Borjas, Ricardo E. and Lynen, Frederic and Sandra, Patrick},
  issn         = {1570-0232},
  journal      = {JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES},
  language     = {eng},
  number       = {1},
  pages        = {248--253},
  title        = {Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2008.07.020},
  volume       = {875},
  year         = {2008},
}

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