Ghent University Academic Bibliography

Advanced

Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time

Deirdre Cabooter, François Lestremau, Frederic Lynen UGent, Patrick Sandra UGent and Gert Desmet (2008) JOURNAL OF CHROMATOGRAPHY A. 1212(1-2). p.23-34
abstract
The present paper reports on the possibility to use the kinetic plot method (KPM) to select and design the best possible system to achieve a given number (100,000) of theoretical plates for a pharmaceutical test mixture, using the information obtained from a series of single Column performance measurements of sub-2 mu m and supra-2 mu m porous shell particles conducted at three different temperatures and using mixtures of acetonitrile and 0.1% formic acid in water as the mobile phase. Because the KPM involves an extrapolation to different column lengths, the quality of the design was subsequently verified by coupling several columns to achieve the optimal total column length and run the actual analysis at the calculated optimal flow rate. The prediction error was generally better than 10%, with a slightly better prediction for to and N than for the retention time t(R). The sub-2 mu m and the porous shell particle coupled column systems achieve the 100,000 plates about equally fast, despite the fact that the former were used at 1000 bar and the latter only at 600 bar. The high temperature Operation (80 degrees C) yielded the fastest separation in both cases, allowing to reach 100,000 plates for a component eluting at k'=2.5 in only about 15 min.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
JOURNAL OF CHROMATOGRAPHY A
J. Chromatogr. A
volume
1212
issue
1-2
pages
23 - 34
Web of Science type
Article
Web of Science id
000261272800004
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.756 (2008)
JCR rank
6/70 (2008)
JCR quartile
1 (2008)
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.09.106
language
English
UGent publication?
yes
classification
A1
id
667700
handle
http://hdl.handle.net/1854/LU-667700
date created
2009-05-27 09:35:29
date last changed
2010-05-12 13:40:53
@article{667700,
  abstract     = {The present paper reports on the possibility to use the kinetic plot method (KPM) to select and design the best possible system to achieve a given number (100,000) of theoretical plates for a pharmaceutical test mixture, using the information obtained from a series of single Column performance measurements of sub-2 mu m and supra-2 mu m porous shell particles conducted at three different temperatures and using mixtures of acetonitrile and 0.1\% formic acid in water as the mobile phase. Because the KPM involves an extrapolation to different column lengths, the quality of the design was subsequently verified by coupling several columns to achieve the optimal total column length and run the actual analysis at the calculated optimal flow rate. The prediction error was generally better than 10\%, with a slightly better prediction for to and N than for the retention time t(R). The sub-2 mu m and the porous shell particle coupled column systems achieve the 100,000 plates about equally fast, despite the fact that the former were used at 1000 bar and the latter only at 600 bar. The high temperature Operation (80 degrees C) yielded the fastest separation in both cases, allowing to reach 100,000 plates for a component eluting at k'=2.5 in only about 15 min.},
  author       = {Cabooter, Deirdre and Lestremau, Fran\c{c}ois and Lynen, Frederic and Sandra, Patrick and Desmet, Gert},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  number       = {1-2},
  pages        = {23--34},
  title        = {Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.09.106},
  volume       = {1212},
  year         = {2008},
}

Chicago
Cabooter, Deirdre, François Lestremau, Frederic Lynen, Patrick Sandra, and Gert Desmet. 2008. “Kinetic Plot Method as a Tool to Design Coupled Column Systems Producing 100,000 Theoretical Plates in the Shortest Possible Time.” Journal of Chromatography A 1212 (1-2): 23–34.
APA
Cabooter, D., Lestremau, F., Lynen, F., Sandra, P., & Desmet, G. (2008). Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time. JOURNAL OF CHROMATOGRAPHY A, 1212(1-2), 23–34.
Vancouver
1.
Cabooter D, Lestremau F, Lynen F, Sandra P, Desmet G. Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time. JOURNAL OF CHROMATOGRAPHY A. 2008;1212(1-2):23–34.
MLA
Cabooter, Deirdre, François Lestremau, Frederic Lynen, et al. “Kinetic Plot Method as a Tool to Design Coupled Column Systems Producing 100,000 Theoretical Plates in the Shortest Possible Time.” JOURNAL OF CHROMATOGRAPHY A 1212.1-2 (2008): 23–34. Print.