Advanced search
1 file | 732.74 KB

Creating a fatty acid methyl ester database for lipid profiling in a single drop of human blood using high resolution capillary gas chromatography and mass spectrometry

(2008) JOURNAL OF CHROMATOGRAPHY A. 1211(1-2). p.120-128
Author
Organization
Abstract
Capillary gas chromatography (CGC) in combination with mass spectrometry (MS) was optimized for the separation and detection of the fatty acids occurring in the lipid fraction of blood. A fingertip blood sample (ca. 50 mu L) was transesterified into the methyl esters and analyzed on a 100 m x 0.25 mm ID column coated with a biscyanopropyl polysiloxane (HP-88) stationary phase. The method was retention time locked. Programmed temperature vaporization injection (PTV) in the solvent venting mode was applied to minimize the sample size, while maintaining high sensitivity. The total analysis time was ca. 60 min. Retention times and both electron impact (EI) and positive chemical ionization (PCI) mass spectrometry were combined to elucidate the fatty acids according to alkyl chain, degree of unsaturation and position of the double bonds. Using extracted ion chromatograms about 100 fatty acids and related compounds Were detected in blood samples and most of them were identified. This work resulted in a very large fatty acid methyl esters database, containing retention time and mass spectral information that will be applied to metabolomic studies.

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 732.74 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Bicalho, Beatriz, Frank David, Klaus Rumplel, Erick Kindt, and Patrick Sandra. 2008. “Creating a Fatty Acid Methyl Ester Database for Lipid Profiling in a Single Drop of Human Blood Using High Resolution Capillary Gas Chromatography and Mass Spectrometry.” Journal of Chromatography A 1211 (1-2): 120–128.
APA
Bicalho, B., David, F., Rumplel, K., Kindt, E., & Sandra, P. (2008). Creating a fatty acid methyl ester database for lipid profiling in a single drop of human blood using high resolution capillary gas chromatography and mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1211(1-2), 120–128.
Vancouver
1.
Bicalho B, David F, Rumplel K, Kindt E, Sandra P. Creating a fatty acid methyl ester database for lipid profiling in a single drop of human blood using high resolution capillary gas chromatography and mass spectrometry. JOURNAL OF CHROMATOGRAPHY A. 2008;1211(1-2):120–8.
MLA
Bicalho, Beatriz, Frank David, Klaus Rumplel, et al. “Creating a Fatty Acid Methyl Ester Database for Lipid Profiling in a Single Drop of Human Blood Using High Resolution Capillary Gas Chromatography and Mass Spectrometry.” JOURNAL OF CHROMATOGRAPHY A 1211.1-2 (2008): 120–128. Print.
@article{667642,
  abstract     = {Capillary gas chromatography (CGC) in combination with mass spectrometry (MS) was optimized for the separation and detection of the fatty acids occurring in the lipid fraction of blood. A fingertip blood sample (ca. 50 mu L) was transesterified into the methyl esters and analyzed on a 100 m x 0.25 mm ID column coated with a biscyanopropyl polysiloxane (HP-88) stationary phase. The method was retention time locked. Programmed temperature vaporization injection (PTV) in the solvent venting mode was applied to minimize the sample size, while maintaining high sensitivity. The total analysis time was ca. 60 min. Retention times and both electron impact (EI) and positive chemical ionization (PCI) mass spectrometry were combined to elucidate the fatty acids according to alkyl chain, degree of unsaturation and position of the double bonds. Using extracted ion chromatograms about 100 fatty acids and related compounds Were detected in blood samples and most of them were identified. This work resulted in a very large fatty acid methyl esters database, containing retention time and mass spectral information that will be applied to metabolomic studies.},
  author       = {Bicalho, Beatriz and David, Frank and Rumplel, Klaus and Kindt, Erick and Sandra, Patrick},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  number       = {1-2},
  pages        = {120--128},
  title        = {Creating a fatty acid methyl ester database for lipid profiling in a single drop of human blood using high resolution capillary gas chromatography and mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.09.066},
  volume       = {1211},
  year         = {2008},
}

Altmetric
View in Altmetric
Web of Science
Times cited: