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Concentration-dependent peptide losses during the drying step in the sample preparation: influence of additives

Adel Pezeshki (UGent) , Valentijn Vergote (UGent) , Christian Burvenich (UGent) and Bart De Spiegeleer (UGent)
(2009)
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Abstract
It is expected that bovine teat canal, teat cistern and/or mammary epithelial cells produce antimicrobial peptides as the first line of defense against pathogens, especially during dry period [1]. Peptide profiling of udder tissues will give us more information about the possible roles of peptides (peptidomics). However, variable recovery of peptides during sample preparation is a well-known phenomenon. Peptide loss, mainly due to adsorption, can occur during sample treatment [2]. Removal of the solvent from samples by drying (both centrifugal evaporation and freeze-drying) was found to significantly reduce the recovery of model peptides [2]. For both drying methods, recovery of the model peptides was increased when 5% v/v of n-nonyl-β-D-glucopyranoside (0.05% w/v aqueous solution) was used as a solution additive. Moreover, applying this additive gave more robust results, i.e. less influenced by the operational variables. Adsorption of peptides is a concentration dependent surface phenomenon. Investigating the concentration effect (200, 100, 20 and 2 μg/ml each) on model peptides adsorption, i.e. bovine insulin, mouse obestatin, goserelin, buserelin and leucine enkephalin, showed that at lower concentrations, higher loss of peptides is occurred. Modeled results based on Langmuir and Fruendlich equations confirmed that concentration effect can be adequately described by the Freundlich equation in our study [2].
Keywords
peptide adsorption, n-nonyl-β-D-glucopyranoside, Bovine mammary gland, peptidomics

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Citation

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Chicago
Pezeshki, Adel, Valentijn Vergote, Christian Burvenich, and Bart De Spiegeleer. 2009. “Concentration-dependent Peptide Losses During the Drying Step in the Sample Preparation: Influence of Additives.” In .
APA
Pezeshki, A., Vergote, V., Burvenich, C., & De Spiegeleer, B. (2009). Concentration-dependent peptide losses during the drying step in the sample preparation: influence of additives. Presented at the Symposium Farmaceutische Wetenschappen.
Vancouver
1.
Pezeshki A, Vergote V, Burvenich C, De Spiegeleer B. Concentration-dependent peptide losses during the drying step in the sample preparation: influence of additives. 2009.
MLA
Pezeshki, Adel et al. “Concentration-dependent Peptide Losses During the Drying Step in the Sample Preparation: Influence of Additives.” 2009. Print.
@inproceedings{666745,
  abstract     = {It is expected that bovine teat canal, teat cistern and/or mammary epithelial cells produce antimicrobial peptides as the first line of defense against pathogens, especially during dry period [1]. Peptide profiling of udder tissues will give us more information about the possible roles of peptides (peptidomics). However, variable recovery of peptides during sample preparation is a well-known phenomenon. Peptide loss, mainly due to adsorption, can occur during sample treatment [2]. Removal of the solvent from samples by drying (both centrifugal evaporation and freeze-drying) was found to significantly reduce the recovery of model peptides [2]. For both drying methods, recovery of the model peptides was increased when 5% v/v of n-nonyl-β-D-glucopyranoside (0.05% w/v aqueous solution) was used as a solution additive. Moreover, applying this additive gave more robust results, i.e. less influenced by the operational variables. Adsorption of peptides is a concentration dependent surface phenomenon. Investigating the concentration effect (200, 100, 20 and 2 μg/ml each) on model peptides adsorption, i.e. bovine insulin, mouse obestatin, goserelin, buserelin and leucine enkephalin, showed that at lower concentrations, higher loss of peptides is occurred. Modeled results based on Langmuir and Fruendlich equations confirmed that concentration effect can be adequately described by the Freundlich equation in our study [2].},
  author       = {Pezeshki, Adel and Vergote, Valentijn and Burvenich, Christian and De Spiegeleer, Bart},
  keywords     = {peptide adsorption,n-nonyl-β-D-glucopyranoside,Bovine mammary gland,peptidomics},
  language     = {eng},
  title        = {Concentration-dependent peptide losses during the drying step in the sample preparation: influence of additives},
  year         = {2009},
}