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Feeding Artemia franciscana (Kellogg) larvae with bacterial heat shock protein, protects from Virbrio campbellii infection

(2009) JOURNAL OF FISH DISEASES. 32(8). p.675-685
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Abstract
Among their numerous physiological effects, heat shock proteins (Hsps) are potent immunomodulators, a characteristic reflecting their potential as therapeutic agents and which led to their application in combating infection. As an example, the up-regulation of endogenous Hsp70 in the branchiopod crustacean Artemia franciscana (Kellogg) is concurrent with shielding against bacterial infection. To better understand this protective mechanism, gnotobiotic Artemia were fed with Escherichia coli treated to over-produce different prokaryotic Hsps. This was shown to increase larval resistance to experimental Vibrio campbellii exposure. Immunoprobing of Western blots showed that the enhanced resistance to V. campbellii correlated with DnaK production in E coli. A definitive role for DnaK was then demonstrated by feeding Artemia larvae with transformed bacteria over-producing only this protein, although other Hsps such as DnaJ and grpE also provided tolerance against Vibrio infection. Feeding of bacteria synthesizing selected Hsps is therefore suggested as an alternative to antibiotic use as a means of enhancing resistance of Artemia larvae to bacterial infection, which may have potential applications in aquaculture.
Keywords
DnaK, Artemia, heat shock proteins, Vibrio, GNOTOBIOTICALLY-GROWN ARTEMIA, LACTIC-ACID BACTERIA, BRINE SHRIMP ARTEMIA, CELL-WALL MUTANTS, PROBIOTIC BACTERIA, ESCHERICHIA-COLI, PENAEUS-MONODON, ANTIBIOTIC-RESISTANCE, PATHOGENIC BACTERIA, IMMUNE-RESPONSE

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Citation

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Chicago
Yik Sung, Yeong, MF Ashame, S Chen, Thomas H MacRae, Patrick Sorgeloos, and Peter Bossier. 2009. “Feeding Artemia Franciscana (Kellogg) Larvae with Bacterial Heat Shock Protein, Protects from Virbrio Campbellii Infection.” Journal of Fish Diseases 32 (8): 675–685.
APA
Yik Sung, Y., Ashame, M., Chen, S., MacRae, T. H., Sorgeloos, P., & Bossier, P. (2009). Feeding Artemia franciscana (Kellogg) larvae with bacterial heat shock protein, protects from Virbrio campbellii infection. JOURNAL OF FISH DISEASES, 32(8), 675–685.
Vancouver
1.
Yik Sung Y, Ashame M, Chen S, MacRae TH, Sorgeloos P, Bossier P. Feeding Artemia franciscana (Kellogg) larvae with bacterial heat shock protein, protects from Virbrio campbellii infection. JOURNAL OF FISH DISEASES. 2009;32(8):675–85.
MLA
Yik Sung, Yeong et al. “Feeding Artemia Franciscana (Kellogg) Larvae with Bacterial Heat Shock Protein, Protects from Virbrio Campbellii Infection.” JOURNAL OF FISH DISEASES 32.8 (2009): 675–685. Print.
@article{664924,
  abstract     = {Among their numerous physiological effects, heat shock proteins (Hsps) are potent immunomodulators, a characteristic reflecting their potential as therapeutic agents and which led to their application in combating infection. As an example, the up-regulation of endogenous Hsp70 in the branchiopod crustacean Artemia franciscana (Kellogg) is concurrent with shielding against bacterial infection. To better understand this protective mechanism, gnotobiotic Artemia were fed with Escherichia coli treated to over-produce different prokaryotic Hsps. This was shown to increase larval resistance to experimental Vibrio campbellii exposure. Immunoprobing of Western blots showed that the enhanced resistance to V. campbellii correlated with DnaK production in E coli. A definitive role for DnaK was then demonstrated by feeding Artemia larvae with transformed bacteria over-producing only this protein, although other Hsps such as DnaJ and grpE also provided tolerance against Vibrio infection. Feeding of bacteria synthesizing selected Hsps is therefore suggested as an alternative to antibiotic use as a means of enhancing resistance of Artemia larvae to bacterial infection, which may have potential applications in aquaculture.},
  author       = {Yik Sung, Yeong and Ashame, MF and Chen, S and MacRae, Thomas H and Sorgeloos, Patrick and Bossier, Peter},
  issn         = {0140-7775},
  journal      = {JOURNAL OF FISH DISEASES},
  keywords     = {DnaK,Artemia,heat shock proteins,Vibrio,GNOTOBIOTICALLY-GROWN ARTEMIA,LACTIC-ACID BACTERIA,BRINE SHRIMP ARTEMIA,CELL-WALL MUTANTS,PROBIOTIC BACTERIA,ESCHERICHIA-COLI,PENAEUS-MONODON,ANTIBIOTIC-RESISTANCE,PATHOGENIC BACTERIA,IMMUNE-RESPONSE},
  language     = {eng},
  number       = {8},
  pages        = {675--685},
  title        = {Feeding Artemia franciscana (Kellogg) larvae with bacterial heat shock protein, protects from Virbrio campbellii infection},
  url          = {http://dx.doi.org/10.1111/j.1365-2761.2009.01046.x},
  volume       = {32},
  year         = {2009},
}

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