@article{664840,
  abstract     = {{Given the key role of interleukin-5 (IL-5) in eosinophil function, we investigated the regulated expression of the membrane-anchored (TM-IL-5R alpha) isoform, or a secreted (SOL IL-5R alpha) isoform, on both protein and transcript level in vitro and in vivo.
A real-time PCR, FACS and ELISA were established to determine IL-5R alpha isoform expression in peripheral blood and nasal tissue from control subjects and nasal polyp (NP) patients with or without asthma. Human peripheral blood eosinophils were incubated with IL-5 and were analyzed for SOL-IL-5R alpha and TM-IL-5R alpha mRNA and protein levels in comparison with CD-69 expression.

SOL-IL-5R alpha and TM-IL-5R alpha mRNA and protein expression was significantly increased in NP vs controls. In polyp tissue, SOL-IL-5R alpha expression correlated to disease severity and eosinophils counts, whereas TM-IL-5R alpha levels were inversely correlated to eosinophils counts and SOL-IL-5R alpha expression. FACS analysis revealed increased CD-69 and decreased TM-IL-5R alpha expression in NP tissue eosinophils vs blood eosinophils. Incubation of blood eosinophils with IL-5 caused up-regulation of CD-69 and down-regulation of TM-IL-5R alpha after 2 and 24 h.

The expression of SOL-IL-5R alpha and TM-IL-5R alpha differs according to the eosinophil activation state and localization in the body (blood vs tissue) and may therefore be involved in the fine-tuning of the eosinophil homeostasis. Exposure of eosinophils to IL-5 reduces their responsiveness to IL-5 by regulated expression of the IL-5R alpha isoforms. Since, TM-IL-5R alpha is down-regulated and SOL-IL-5R alpha (antagonistic) is upregulated in NP tissue, our findings are important to understand the clinical trials with anti-IL-5 in humans.}},
  author       = {{Gevaert, Philippe and Hellman, C. and Lundblad, L. and Lundahl, J. and Holtappels, Gabriële and Van Cauwenberge, Paul and Tavernier, Jan and Bachert, Claus}},
  issn         = {{0105-4538}},
  journal      = {{ALLERGY}},
  keywords     = {{rhinosinusitis,KeyWords Plus: SOLUBLE IL-5 RECEPTOR,DECREASED EXPRESSION,ALLERGEN CHALLENGE,GM-CSF,INHIBITION,SUBUNIT,INFLAMMATION,CYTOKINES,AIRWAY,CHAIN,nasal polyps,eosinophils,IL-5 receptor alpha}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{725--732}},
  publisher    = {{WILEY-BLACKWELL PUBLISHING, INC}},
  title        = {{Differential expression of the interleukin 5 receptor alpha isoforms in blood and tissue eosinophils of nasal polyp patients}},
  url          = {{http://doi.org/10.1111/j.1398-9995.2008.01885.x}},
  volume       = {{64}},
  year         = {{2009}},
}

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