Advanced search
2 files | 1.66 MB

Monitoring ALS1 and ALS3 Gene Expression During In Vitro Candida albicans Biofilm Formation Under Continuous Flow Conditions

Heleen Nailis (UGent) , Roosmarijn Vandenbroucke (UGent) , Kelly Tilleman (UGent) , Dieter Deforce (UGent) , Hans Nelis (UGent) and Tom Coenye (UGent)
(2009) MYCOPATHOLOGIA. 167(1). p.9-17
Author
Organization
Abstract
Abstract: ALS1 and ALS3 encode cell-surface associated glycoproteins that are considered to be important for Candida albicans biofilm formation. The main goal of the present study was to monitor ALS1 and ALS3 gene expression during C. albicans biofilm formation (on silicone) under continuous flow conditions, using the Centers for Disease Control biofilm reactor (CDC reactor). For ALS1, we found few changes in gene expression until later stages of biofilm formation (72 and 96 h) when this gene appeared to be downregulated relative to the gene expression level in the start culture. We observed an induction of ALS3 gene expression in the initial stages of biofilm formation (0.5, 1, and 6 h), whereas at later stages, this gene was also downregulated relative to the gene expression level in the start culture. We also found that biofilms of an als3/als3 deletion mutant contained less filaments at several time points (1, 6, 24, and 48 h), although filamentation as such was not affected in this strain. Together, our data indicate an important role for ALS3 in the early phases of biofilm formation in the CDC reactor, probably related to adhesion of filaments, while the role of ALS1 is less clear.
Keywords
SURFACES, REAL-TIME, FAMILY, MODEL, FILAMENTATION, RESISTANCE, ADHESIN, QUANTIFICATION, PCR

Downloads

  • 664748-text.doc
    • full text
    • |
    • open access
    • |
    • application/x-ole-storage
    • |
    • 115.20 KB
  • 664748-figs.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.54 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Nailis, Heleen, Roosmarijn Vandenbroucke, Kelly Tilleman, Dieter Deforce, Hans Nelis, and Tom Coenye. 2009. “Monitoring ALS1 and ALS3 Gene Expression During In Vitro Candida Albicans Biofilm Formation Under Continuous Flow Conditions.” Mycopathologia 167 (1): 9–17.
APA
Nailis, H., Vandenbroucke, R., Tilleman, K., Deforce, D., Nelis, H., & Coenye, T. (2009). Monitoring ALS1 and ALS3 Gene Expression During In Vitro Candida albicans Biofilm Formation Under Continuous Flow Conditions. MYCOPATHOLOGIA, 167(1), 9–17.
Vancouver
1.
Nailis H, Vandenbroucke R, Tilleman K, Deforce D, Nelis H, Coenye T. Monitoring ALS1 and ALS3 Gene Expression During In Vitro Candida albicans Biofilm Formation Under Continuous Flow Conditions. MYCOPATHOLOGIA. 2009;167(1):9–17.
MLA
Nailis, Heleen et al. “Monitoring ALS1 and ALS3 Gene Expression During In Vitro Candida Albicans Biofilm Formation Under Continuous Flow Conditions.” MYCOPATHOLOGIA 167.1 (2009): 9–17. Print.
@article{664748,
  abstract     = {Abstract: ALS1 and ALS3 encode cell-surface associated glycoproteins that are considered to be important for Candida albicans biofilm formation. The main goal of the present study was to monitor ALS1 and ALS3 gene expression during C. albicans biofilm formation (on silicone) under continuous flow conditions, using the Centers for Disease Control biofilm reactor (CDC reactor). For ALS1, we found few changes in gene expression until later stages of biofilm formation (72 and 96 h) when this gene appeared to be downregulated relative to the gene expression level in the start culture. We observed an induction of ALS3 gene expression in the initial stages of biofilm formation (0.5, 1, and 6 h), whereas at later stages, this gene was also downregulated relative to the gene expression level in the start culture. We also found that biofilms of an als3/als3 deletion mutant contained less filaments at several time points (1, 6, 24, and 48 h), although filamentation as such was not affected in this strain. Together, our data indicate an important role for ALS3 in the early phases of biofilm formation in the CDC reactor, probably related to adhesion of filaments, while the role of ALS1 is less clear.},
  author       = {Nailis, Heleen and Vandenbroucke, Roosmarijn and Tilleman, Kelly and Deforce, Dieter and Nelis, Hans and Coenye, Tom},
  issn         = {0301-486X},
  journal      = {MYCOPATHOLOGIA},
  keywords     = {SURFACES,REAL-TIME,FAMILY,MODEL,FILAMENTATION,RESISTANCE,ADHESIN,QUANTIFICATION,PCR},
  language     = {eng},
  number       = {1},
  pages        = {9--17},
  title        = {Monitoring ALS1 and ALS3 Gene Expression During In Vitro Candida albicans Biofilm Formation Under Continuous Flow Conditions},
  url          = {http://dx.doi.org/10.1007/s11046-608-91148-6},
  volume       = {167},
  year         = {2009},
}

Altmetric
View in Altmetric
Web of Science
Times cited: