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Heifer and quarter characteristics associated with periparturient blood and milk neutrophil apoptosis in healthy heifers and in heifers with subclinical mastitis

Sofie Piepers UGent, Geert Opsomer UGent, Evelyne Meyer UGent, Kristel Demeyere UGent, Herman Barkema UGent, Aart de Kruif UGent and Sarne De Vliegher UGent (2009) JOURNAL OF DAIRY SCIENCE. 92(9). p.4330-4339
abstract
Polymorphonuclear neutrophilic leukocytes (PMN) play an important role in the first line cell-mediated immune defense of the body in general and of the mammary gland against mastitis pathogens in particular. Reduced viability of PMN close to parturition may explain the high incidence of infectious diseases and the high prevalence of intramammary infections (IMI) in periparturient dairy heifers. Apoptosis of blood PMN 1 wk before the expected calving date and of blood and milk PMN at 1 to 4 d in milk was determined using flow cytometry. Information on heifer and gland characteristics was collected before calving and in early lactation. Data were analyzed using multivariable, multilevel regression analysis. Supplementation of a commercial mineral/vitamin mix prior to calving was associated with less blood (14.4 ± 2.9% versus 22.4 ± 2.1%) and milk PMN apoptosis (19.0 ± 1.1% versus 26.4 ± 0.9%) near calving, presumably related to higher blood selenium concentrations. Both blood and milk PMN apoptosis showed a seasonal variation with the highest proportion of apoptotic cells between January and March (32.0 ± 6.1% and 34.6 ± 2.7%, respectively) and April and June (31.3 ± 5.7% and 37.8 ± 2.3%, respectively). Heifers losing more than 0.25 points of their body condition (BCS) in the periparturient period had higher proportions of apoptotic blood PMN in early lactation compared to heifers losing less than 0.25 points (24.0 ± 2.8% versus 16.6 ± 1.7%). Milk PMN apoptosis was less pronounced in quarters having teat orifices colonized with non-aureus staphylococci prior to calving (18.9 ± 1.0% versus 29.4 ± 1.0%). The variation in blood PMN apoptosis before and after calving mainly resided at the heifer level (71.4 and 98.4% of the total variation, respectively), whereas the variation in milk PMN apoptosis mainly resided at the heifer (45.7% of the total variation) and quarter level (45.5% of the total variation). These data imply that the impaired blood and milk PMN viability in periparturient heifers can be reduced by optimization of certain heifer management practices such as supplementation of minerals/vitamins, and pasture and feeding strategies.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
COWS, RISK-FACTORS, MANAGEMENT-PRACTICES, NEGATIVE STAPHYLOCOCCI, early lactation, neutrophil apoptosis, DAIRY HEIFERS, milk, heifer, SOMATIC-CELL COUNT, EARLY LACTATION, REPRODUCTIVE-PERFORMANCE, INTRAMAMMARY INFECTIONS, STAPHYLOCOCCUS-CHROMOGENES
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
92
issue
9
pages
4330 - 4339
Web of Science type
Article
Web of Science id
000269156600022
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.463 (2009)
JCR rank
2/49 (2009)
JCR quartile
1 (2009)
ISSN
0022-0302
DOI
10.3168/jds.2009-2029
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
663541
handle
http://hdl.handle.net/1854/LU-663541
date created
2009-05-24 19:40:10
date last changed
2012-10-29 16:51:34
@article{663541,
  abstract     = {Polymorphonuclear neutrophilic leukocytes (PMN) play an important role in the first line cell-mediated immune defense of the body in general and of the mammary gland against mastitis pathogens in particular. Reduced viability of PMN close to parturition may explain the high incidence of infectious diseases and the high prevalence of intramammary infections (IMI) in periparturient dairy heifers. Apoptosis of blood PMN 1 wk before the expected calving date and of blood and milk PMN at 1 to 4 d in milk was determined using flow cytometry. Information on heifer and gland characteristics was collected before calving and in early lactation. Data were analyzed using multivariable, multilevel regression analysis. Supplementation of a commercial mineral/vitamin mix prior to calving was associated with less blood (14.4 {\textpm} 2.9\% versus 22.4 {\textpm} 2.1\%) and milk PMN apoptosis (19.0 {\textpm} 1.1\% versus 26.4 {\textpm} 0.9\%) near calving, presumably related to higher blood selenium concentrations. Both blood and milk PMN apoptosis showed a seasonal variation with the highest proportion of apoptotic cells between January and March (32.0 {\textpm} 6.1\% and 34.6 {\textpm} 2.7\%, respectively) and April and June (31.3 {\textpm} 5.7\% and 37.8 {\textpm} 2.3\%, respectively). Heifers losing more than 0.25 points of their body condition (BCS) in the periparturient period had higher proportions of apoptotic blood PMN in early lactation compared to heifers losing less than 0.25 points (24.0 {\textpm} 2.8\% versus 16.6 {\textpm} 1.7\%). Milk PMN apoptosis was less pronounced in quarters having teat orifices colonized with non-aureus staphylococci prior to calving (18.9 {\textpm} 1.0\% versus 29.4 {\textpm} 1.0\%). The variation in blood PMN apoptosis before and after calving mainly resided at the heifer level (71.4 and 98.4\% of the total variation, respectively), whereas the variation in milk PMN apoptosis mainly resided at the heifer (45.7\% of the total variation) and quarter level (45.5\% of the total variation). These data imply that the impaired blood and milk PMN viability in periparturient heifers can be reduced by optimization of certain heifer management practices such as supplementation of minerals/vitamins, and pasture and feeding strategies.},
  author       = {Piepers, Sofie and Opsomer, Geert and Meyer, Evelyne and Demeyere, Kristel and Barkema, Herman and de Kruif, Aart and De Vliegher, Sarne},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {COWS,RISK-FACTORS,MANAGEMENT-PRACTICES,NEGATIVE STAPHYLOCOCCI,early lactation,neutrophil apoptosis,DAIRY HEIFERS,milk,heifer,SOMATIC-CELL COUNT,EARLY LACTATION,REPRODUCTIVE-PERFORMANCE,INTRAMAMMARY INFECTIONS,STAPHYLOCOCCUS-CHROMOGENES},
  language     = {eng},
  number       = {9},
  pages        = {4330--4339},
  title        = {Heifer and quarter characteristics associated with periparturient blood and milk neutrophil apoptosis in healthy heifers and in heifers with subclinical mastitis},
  url          = {http://dx.doi.org/10.3168/jds.2009-2029},
  volume       = {92},
  year         = {2009},
}

Chicago
Piepers, Sofie, Geert Opsomer, Evelyne Meyer, Kristel Demeyere, Herman Barkema, Aart de Kruif, and Sarne De Vliegher. 2009. “Heifer and Quarter Characteristics Associated with Periparturient Blood and Milk Neutrophil Apoptosis in Healthy Heifers and in Heifers with Subclinical Mastitis.” Journal of Dairy Science 92 (9): 4330–4339.
APA
Piepers, S., Opsomer, G., Meyer, E., Demeyere, K., Barkema, H., de Kruif, A., & De Vliegher, S. (2009). Heifer and quarter characteristics associated with periparturient blood and milk neutrophil apoptosis in healthy heifers and in heifers with subclinical mastitis. JOURNAL OF DAIRY SCIENCE, 92(9), 4330–4339.
Vancouver
1.
Piepers S, Opsomer G, Meyer E, Demeyere K, Barkema H, de Kruif A, et al. Heifer and quarter characteristics associated with periparturient blood and milk neutrophil apoptosis in healthy heifers and in heifers with subclinical mastitis. JOURNAL OF DAIRY SCIENCE. 2009;92(9):4330–9.
MLA
Piepers, Sofie, Geert Opsomer, Evelyne Meyer, et al. “Heifer and Quarter Characteristics Associated with Periparturient Blood and Milk Neutrophil Apoptosis in Healthy Heifers and in Heifers with Subclinical Mastitis.” JOURNAL OF DAIRY SCIENCE 92.9 (2009): 4330–4339. Print.