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Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates

(2009) INTERNATIONAL MICROBIOLOGY. 11(4). p.261-266
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Abstract
The probiotic relevant characteristics of 45 strains of vaginal Lactobocillus isolated from healthy women were analyzed. Of these, 21 strains were classified as L. crispatus, 17 as L. jensenii, six as L. gasseri, and one as L. plantarum. The rate of acidification varied significantly between the strains as did their ability to form biofilms. None used glycogen as a fermentable carbohydrate. H(2)O(2) generation was common, especially among L. jensenii isolates (88%). No bacteriocinogenic strains were detected. Most strains harbored plasmids (from 1 to 7) of various sizes, those in excess of 50 kb being frequent. One of these plasmids was found to be promiscuous since it hybridized with extrachromosomal bands of 15 isolates. All strains were resistant to metronidazole, ciprofloxacin, gentamicin, clindamycin, trimethoprim, and sulfametoxazole and susceptible to a series of beta-lactams, erythromycin, tetracycline, and benzalkonium chloride. Almost half of the strains were highly resistant to nonoxinol 9, which is commonly used as a spermicide. Based on these analyses, strains of all three common species are proposed as new probiotic candidates.
Keywords
vaginal lactobacilli, Lactobacillus spp., probiotics, plasmids, antibiotic susceptibility/resistance, spermicides, LACTIC-ACID BACTERIA, TETRACYCLINE RESISTANCE GENE, HYDROGEN-PEROXIDE PRODUCTION, PLACEBO-CONTROLLED TRIAL, EPITHELIAL-CELLS, RHAMNOSUS GR-1, FLORA, IDENTIFICATION, UROPATHOGENS, INFECTIONS

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Citation

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MLA
Martín, Rebeca et al. “Characterization of Indigenous Vaginal Lactobacilli from Healthy Women as Probiotic Candidates.” INTERNATIONAL MICROBIOLOGY 11.4 (2009): 261–266. Print.
APA
Martín, R., Soberón, N., Vaneechoutte, M., Flórez, A. B., Vázquez, F., & Suárez, J. E. (2009). Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates. INTERNATIONAL MICROBIOLOGY, 11(4), 261–266.
Chicago author-date
Martín, Rebeca, Nora Soberón, Mario Vaneechoutte, Ana B Flórez, Fernando Vázquez, and Juan E Suárez. 2009. “Characterization of Indigenous Vaginal Lactobacilli from Healthy Women as Probiotic Candidates.” International Microbiology 11 (4): 261–266.
Chicago author-date (all authors)
Martín, Rebeca, Nora Soberón, Mario Vaneechoutte, Ana B Flórez, Fernando Vázquez, and Juan E Suárez. 2009. “Characterization of Indigenous Vaginal Lactobacilli from Healthy Women as Probiotic Candidates.” International Microbiology 11 (4): 261–266.
Vancouver
1.
Martín R, Soberón N, Vaneechoutte M, Flórez AB, Vázquez F, Suárez JE. Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates. INTERNATIONAL MICROBIOLOGY. 2009;11(4):261–6.
IEEE
[1]
R. Martín, N. Soberón, M. Vaneechoutte, A. B. Flórez, F. Vázquez, and J. E. Suárez, “Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates,” INTERNATIONAL MICROBIOLOGY, vol. 11, no. 4, pp. 261–266, 2009.
@article{628746,
  abstract     = {{The probiotic relevant characteristics of 45 strains of vaginal Lactobocillus isolated from healthy women were analyzed. Of these, 21 strains were classified as L. crispatus, 17 as L. jensenii, six as L. gasseri, and one as L. plantarum. The rate of acidification varied significantly between the strains as did their ability to form biofilms. None used glycogen as a fermentable carbohydrate. H(2)O(2) generation was common, especially among L. jensenii isolates (88%). No bacteriocinogenic strains were detected. Most strains harbored plasmids (from 1 to 7) of various sizes, those in excess of 50 kb being frequent. One of these plasmids was found to be promiscuous since it hybridized with extrachromosomal bands of 15 isolates. All strains were resistant to metronidazole, ciprofloxacin, gentamicin, clindamycin, trimethoprim, and sulfametoxazole and susceptible to a series of beta-lactams, erythromycin, tetracycline, and benzalkonium chloride. Almost half of the strains were highly resistant to nonoxinol 9, which is commonly used as a spermicide. Based on these analyses, strains of all three common species are proposed as new probiotic candidates.}},
  author       = {{Martín, Rebeca and Soberón, Nora and Vaneechoutte, Mario and Flórez, Ana B and Vázquez, Fernando and Suárez, Juan E}},
  issn         = {{1139-6709}},
  journal      = {{INTERNATIONAL MICROBIOLOGY}},
  keywords     = {{vaginal lactobacilli,Lactobacillus spp.,probiotics,plasmids,antibiotic susceptibility/resistance,spermicides,LACTIC-ACID BACTERIA,TETRACYCLINE RESISTANCE GENE,HYDROGEN-PEROXIDE PRODUCTION,PLACEBO-CONTROLLED TRIAL,EPITHELIAL-CELLS,RHAMNOSUS GR-1,FLORA,IDENTIFICATION,UROPATHOGENS,INFECTIONS}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{261--266}},
  title        = {{Characterization of indigenous vaginal lactobacilli from healthy women as probiotic candidates}},
  url          = {{http://dx.doi.org/10.2436/20.1501.01.70}},
  volume       = {{11}},
  year         = {{2009}},
}

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