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Analysis of cytotoxic T cell precursor frequencies directed against individual HLA-A and -B alloantigens

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Abstract
We describe here a limiting dilution analysis to determine cytotoxic T lymphocyte precursor (CTLp) frequencies against individual HLA-A or -B antigens. This assay is reproducible and showed that the CTLp frequency of an individual remains stable with time. Significant variations in CTLp frequency against the same alloantigen were found in different individuals and even in monozygotic twins, showing that these differences were not (completely) genetically determined. Within an individual, a wide range of CTLp frequencies can be found against different allo-antigens. Serologically cross-reactivity seems not to interfere in this assay. This LDA is a practicable tool for a systematic analysis of CTLp response against selected individual HLA-A or -B antigens and can be used for the selection of HLA mismatched donors for transplantation patients.

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Citation

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MLA
Zhang, Li et al. “Analysis of Cytotoxic T Cell Precursor Frequencies Directed Against Individual HLA-A and -B Alloantigens.” JOURNAL OF IMMUNOLOGICAL METHODS 121.1 (1989): 39–45. Print.
APA
Zhang, Li, Li, S. G., Vandekerckhove, B., Termijtelen, A., Van Rood, J. J., & Claas, F. H. (1989). Analysis of cytotoxic T cell precursor frequencies directed against individual HLA-A and -B alloantigens. JOURNAL OF IMMUNOLOGICAL METHODS, 121(1), 39–45.
Chicago author-date
Zhang, Li, Shu Guang Li, Bart Vandekerckhove, Annemarie Termijtelen, Joh J Van Rood, and Frans HJ Claas. 1989. “Analysis of Cytotoxic T Cell Precursor Frequencies Directed Against Individual HLA-A and -B Alloantigens.” Journal of Immunological Methods 121 (1): 39–45.
Chicago author-date (all authors)
Zhang, Li, Shu Guang Li, Bart Vandekerckhove, Annemarie Termijtelen, Joh J Van Rood, and Frans HJ Claas. 1989. “Analysis of Cytotoxic T Cell Precursor Frequencies Directed Against Individual HLA-A and -B Alloantigens.” Journal of Immunological Methods 121 (1): 39–45.
Vancouver
1.
Zhang L, Li SG, Vandekerckhove B, Termijtelen A, Van Rood JJ, Claas FH. Analysis of cytotoxic T cell precursor frequencies directed against individual HLA-A and -B alloantigens. JOURNAL OF IMMUNOLOGICAL METHODS. 1989;121(1):39–45.
IEEE
[1]
L. Zhang, S. G. Li, B. Vandekerckhove, A. Termijtelen, J. J. Van Rood, and F. H. Claas, “Analysis of cytotoxic T cell precursor frequencies directed against individual HLA-A and -B alloantigens,” JOURNAL OF IMMUNOLOGICAL METHODS, vol. 121, no. 1, pp. 39–45, 1989.
@article{628449,
  abstract     = {We describe here a limiting dilution analysis to determine cytotoxic T lymphocyte precursor (CTLp) frequencies against individual HLA-A or -B antigens. This assay is reproducible and showed that the CTLp frequency of an individual remains stable with time. Significant variations in CTLp frequency against the same alloantigen were found in different individuals and even in monozygotic twins, showing that these differences were not (completely) genetically determined. Within an individual, a wide range of CTLp frequencies can be found against different allo-antigens. Serologically cross-reactivity seems not to interfere in this assay. This LDA is a practicable tool for a systematic analysis of CTLp response against selected individual HLA-A or -B antigens and can be used for the selection of HLA mismatched donors for transplantation patients.},
  author       = {Zhang, Li and Li, Shu Guang and Vandekerckhove, Bart and Termijtelen, Annemarie and Van Rood, Joh J and Claas, Frans HJ},
  issn         = {0022-1759},
  journal      = {JOURNAL OF IMMUNOLOGICAL METHODS},
  language     = {eng},
  number       = {1},
  pages        = {39--45},
  title        = {Analysis of cytotoxic T cell precursor frequencies directed against individual HLA-A and -B alloantigens},
  url          = {http://dx.doi.org/10.1016/0022-1759(89)90417-1},
  volume       = {121},
  year         = {1989},
}

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