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Cryotolerance of bovine blastocysts is affected by oocyte maturation in media containing palmitic or stearic acid

Mohamed Ahmed MM Shehab El Deen UGent, JLMR Leroy, Dominiek Maes UGent and Ann Van Soom UGent (2009) REPRODUCTION IN DOMESTIC ANIMALS. 44(1). p.140-142
abstract
In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concentrations measured previously in follicular fluid (FF) of high-producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These in vitro results suggest that high NEFA concentrations in FF during a period of negative energy balance in high-yielding dairy cows can have carry-over effects on embryo quality.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Cryotolerance
journal title
REPRODUCTION IN DOMESTIC ANIMALS
Reprod. Domest. Anim.
volume
44
issue
1
pages
140 - 142
Web of Science type
Article
Web of Science id
000262487500024
ISSN
1439-0531
0963-6768
DOI
10.1111/j.1439-0531.2008.01084.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
627924
handle
http://hdl.handle.net/1854/LU-627924
date created
2009-05-14 15:46:47
date last changed
2009-05-27 10:05:59
@article{627924,
  abstract     = {In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concentrations measured previously in follicular fluid (FF) of high-producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5\% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These in vitro results suggest that high NEFA concentrations in FF during a period of negative energy balance in high-yielding dairy cows can have carry-over effects on embryo quality.},
  author       = {Shehab El Deen, Mohamed Ahmed MM and Leroy, JLMR and Maes, Dominiek and Van Soom, Ann},
  issn         = {1439-0531},
  journal      = {REPRODUCTION IN DOMESTIC ANIMALS},
  keyword      = {Cryotolerance},
  language     = {eng},
  number       = {1},
  pages        = {140--142},
  title        = {Cryotolerance of bovine blastocysts is affected by oocyte maturation in media containing palmitic or stearic acid},
  url          = {http://dx.doi.org/10.1111/j.1439-0531.2008.01084.x},
  volume       = {44},
  year         = {2009},
}

Chicago
Shehab El Deen, Mohamed Ahmed MM, JLMR Leroy, Dominiek Maes, and Ann Van Soom. 2009. “Cryotolerance of Bovine Blastocysts Is Affected by Oocyte Maturation in Media Containing Palmitic or Stearic Acid.” Reproduction in Domestic Animals 44 (1): 140–142.
APA
Shehab El Deen, M. A. M., Leroy, J., Maes, D., & Van Soom, A. (2009). Cryotolerance of bovine blastocysts is affected by oocyte maturation in media containing palmitic or stearic acid. REPRODUCTION IN DOMESTIC ANIMALS, 44(1), 140–142.
Vancouver
1.
Shehab El Deen MAM, Leroy J, Maes D, Van Soom A. Cryotolerance of bovine blastocysts is affected by oocyte maturation in media containing palmitic or stearic acid. REPRODUCTION IN DOMESTIC ANIMALS. 2009;44(1):140–2.
MLA
Shehab El Deen, Mohamed Ahmed MM, JLMR Leroy, Dominiek Maes, et al. “Cryotolerance of Bovine Blastocysts Is Affected by Oocyte Maturation in Media Containing Palmitic or Stearic Acid.” REPRODUCTION IN DOMESTIC ANIMALS 44.1 (2009): 140–142. Print.