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Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples

Sophie Marchand, Kim Heylen UGent, Winy Messens, Katleen Coudijzer, Paul De Vos UGent, Koen Dewettinck UGent, Lieve Herman, Jan De Block and Marc Heyndrickx UGent (2009) ENVIRONMENTAL MICROBIOLOGY. 11(2). p.467-482
abstract
Psychrotolerant bacteria and their heat-resistant proteases play a major role in the spoilage of UHT-processed dairy products. Summer and winter raw milk samples were screened for the presence of such bacteria. One hundred and three proteolytic psychrotolerant bacteria were isolated, characterized by API tests, rep-PCR fingerprint analysis and evaluated for heat-resistant protease production. Twenty-nine strains (representing 79% of the complete collection) were further identified by 16S rRNA gene sequencing, rpoB gene sequencing and DNA-DNA hybridizations. A seasonal inter- and intra-species influence on milk spoilage capacity (e.g. growth rate and/or protease production) was demonstrated. Moreover, this polyphasic approach led to the identification of Pseudomonas fragi and Pseudomonas lundensis (representing 53% of all isolates) as predominant producers of heat-resistant proteases in raw milk. The role of Pseudomonas fluorescens, historically reported as important milk spoiler, could not unequivocally be established. The use of more reliable identification techniques and further revision of the taxonomy of P. fluorescens will probably result in a different perspective on its role in the milk spoilage issue.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PHASE VARIATION, FATTY-ACIDS, GENUS PSEUDOMONAS, ANTIBACTERIAL ACTIVITY, FIMBRIAL TRANSCRIPTION, ESCHERICHIA-COLI, DAIRY-PRODUCTS, SP-NOV., DEOXYRIBONUCLEIC-ACID, POLYMERASE-CHAIN-REACTION
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
volume
11
issue
2
pages
467 - 482
Web of Science type
Article
Web of Science id
000262515800016
JCR category
MICROBIOLOGY
JCR impact factor
4.909 (2009)
JCR rank
15/94 (2009)
JCR quartile
1 (2009)
ISSN
1462-2912
DOI
10.1111/j.1462-2920.2008.01785.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
626934
handle
http://hdl.handle.net/1854/LU-626934
date created
2009-05-13 18:07:19
date last changed
2012-11-12 09:28:58
@article{626934,
  abstract     = {Psychrotolerant bacteria and their heat-resistant proteases play a major role in the spoilage of UHT-processed dairy products. Summer and winter raw milk samples were screened for the presence of such bacteria. One hundred and three proteolytic psychrotolerant bacteria were isolated, characterized by API tests, rep-PCR fingerprint analysis and evaluated for heat-resistant protease production. Twenty-nine strains (representing 79\% of the complete collection) were further identified by 16S rRNA gene sequencing, rpoB gene sequencing and DNA-DNA hybridizations. A seasonal inter- and intra-species influence on milk spoilage capacity (e.g. growth rate and/or protease production) was demonstrated. Moreover, this polyphasic approach led to the identification of Pseudomonas fragi and Pseudomonas lundensis (representing 53\% of all isolates) as predominant producers of heat-resistant proteases in raw milk. The role of Pseudomonas fluorescens, historically reported as important milk spoiler, could not unequivocally be established. The use of more reliable identification techniques and further revision of the taxonomy of P. fluorescens will probably result in a different perspective on its role in the milk spoilage issue.},
  author       = {Marchand, Sophie and Heylen, Kim and Messens, Winy and Coudijzer, Katleen and De Vos, Paul and Dewettinck, Koen and Herman, Lieve and De Block, Jan and Heyndrickx, Marc},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {PHASE VARIATION,FATTY-ACIDS,GENUS PSEUDOMONAS,ANTIBACTERIAL ACTIVITY,FIMBRIAL TRANSCRIPTION,ESCHERICHIA-COLI,DAIRY-PRODUCTS,SP-NOV.,DEOXYRIBONUCLEIC-ACID,POLYMERASE-CHAIN-REACTION},
  language     = {eng},
  number       = {2},
  pages        = {467--482},
  title        = {Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2008.01785.x},
  volume       = {11},
  year         = {2009},
}

Chicago
Marchand, Sophie, Kim Heylen, Winy Messens, Katleen Coudijzer, Paul De Vos, Koen Dewettinck, Lieve Herman, Jan De Block, and Marc Heyndrickx. 2009. “Seasonal Influence on Heat-resistant Proteolytic Capacity of Pseudomonas Lundensis and Pseudomonas Fragi, Predominant Milk Spoilers Isolated from Belgian Raw Milk Samples.” Environmental Microbiology 11 (2): 467–482.
APA
Marchand, S., Heylen, K., Messens, W., Coudijzer, K., De Vos, P., Dewettinck, K., Herman, L., et al. (2009). Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples. ENVIRONMENTAL MICROBIOLOGY, 11(2), 467–482.
Vancouver
1.
Marchand S, Heylen K, Messens W, Coudijzer K, De Vos P, Dewettinck K, et al. Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples. ENVIRONMENTAL MICROBIOLOGY. 2009;11(2):467–82.
MLA
Marchand, Sophie, Kim Heylen, Winy Messens, et al. “Seasonal Influence on Heat-resistant Proteolytic Capacity of Pseudomonas Lundensis and Pseudomonas Fragi, Predominant Milk Spoilers Isolated from Belgian Raw Milk Samples.” ENVIRONMENTAL MICROBIOLOGY 11.2 (2009): 467–482. Print.