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Induction of isotype switching and IG production by CD5+ and CD10+ human fetal B-cells

(1992) JOURNAL OF IMMUNOLOGY. 148(11). p.3398-3404
Author
Organization
Abstract
In the present study the capacity of early fetal B cells to produce Ig was investigated. It is shown that B cells from fetal liver, spleen, and bone marrow (BM) can be induced to produce IgM, IgG, IgG4, and IgE, but not IgA, in response to IL-4 in the presence of anti-CD40 mAb or cloned CD4+ T cells. Even splenic B cells from a human fetus of only 12 wk of gestation produced these Ig isotypes. IFN-alpha, IFN-gamma, and transforming growth factor-beta inhibited IL-4-induced IgE production in fetal B cells, as described for mature B cells. The majority of B cells in fetal spleen expressed CD5 and CD10 and > 99% of B cells in fetal BM were CD10+. Highly purified CD10+, CD19+ immature B cells and CD5+, CD19+ B cells could be induced to produce Ig, including IgG4 and IgE, in similar amounts as unseparated CD19+ B cells. Virtually all CD19+ cells still expressed CD10 after 12 days of culture. However, the IgE-producing cells at the end of the culture period were found in the CD19-,CD10- cell population, suggesting differentiation of CD19+, CD10+ B cells into CD19-, CD10- plasma cells. Pre-B cells are characterized by their lack of expression of surface IgM (sIgM). Only 30 to 40% of BM B cells expressed sIgM. However, in contrast to sIgM+, CD10+, CD19+ immature B cells, sorted sIgM-, CD10+, CD19+ pre-B cells failed to differentiate into Ig-secreting cells under the present culture conditions. Addition of IL-6 to these cultures was ineffective. Taken together, these results indicate that fetal CD5+ and CD10+ B cells are mature in their capacity to be induced to Ig isotype switching in vitro as soon as they express sIgM.
Keywords
INTERLEUKIN-4, ANTIGEN, PRE-B, POKEWEED MITOGEN, MONONUCLEAR-CELLS, LYMPHOCYTES, STAPHYLOCOCCUS-AUREUS, ACUTE LYMPHOBLASTIC-LEUKEMIA, INVITRO, PRECURSORS

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Chicago
Punnonen, J, G Aversa, Bart Vandekerckhove, M Roncarolo, and J De Vries. 1992. “Induction of Isotype Switching and IG Production by CD5+ and CD10+ Human Fetal B-cells.” Journal of Immunology 148 (11): 3398–3404.
APA
Punnonen, J., Aversa, G., Vandekerckhove, B., Roncarolo, M., & De Vries, J. (1992). Induction of isotype switching and IG production by CD5+ and CD10+ human fetal B-cells. JOURNAL OF IMMUNOLOGY, 148(11), 3398–3404.
Vancouver
1.
Punnonen J, Aversa G, Vandekerckhove B, Roncarolo M, De Vries J. Induction of isotype switching and IG production by CD5+ and CD10+ human fetal B-cells. JOURNAL OF IMMUNOLOGY. 1992;148(11):3398–404.
MLA
Punnonen, J et al. “Induction of Isotype Switching and IG Production by CD5+ and CD10+ Human Fetal B-cells.” JOURNAL OF IMMUNOLOGY 148.11 (1992): 3398–3404. Print.
@article{626560,
  abstract     = {In the present study the capacity of early fetal B cells to produce Ig was investigated. It is shown that B cells from fetal liver, spleen, and bone marrow (BM) can be induced to produce IgM, IgG, IgG4, and IgE, but not IgA, in response to IL-4 in the presence of anti-CD40 mAb or cloned CD4+ T cells. Even splenic B cells from a human fetus of only 12 wk of gestation produced these Ig isotypes. IFN-alpha, IFN-gamma, and transforming growth factor-beta inhibited IL-4-induced IgE production in fetal B cells, as described for mature B cells. The majority of B cells in fetal spleen expressed CD5 and CD10 and > 99% of B cells in fetal BM were CD10+. Highly purified CD10+, CD19+ immature B cells and CD5+, CD19+ B cells could be induced to produce Ig, including IgG4 and IgE, in similar amounts as unseparated CD19+ B cells. Virtually all CD19+ cells still expressed CD10 after 12 days of culture. However, the IgE-producing cells at the end of the culture period were found in the CD19-,CD10- cell population, suggesting differentiation of CD19+, CD10+ B cells into CD19-, CD10- plasma cells. Pre-B cells are characterized by their lack of expression of surface IgM (sIgM). Only 30 to 40% of BM B cells expressed sIgM. However, in contrast to sIgM+, CD10+, CD19+ immature B cells, sorted sIgM-, CD10+, CD19+ pre-B cells failed to differentiate into Ig-secreting cells under the present culture conditions. Addition of IL-6 to these cultures was ineffective. Taken together, these results indicate that fetal CD5+ and CD10+ B cells are mature in their capacity to be induced to Ig isotype switching in vitro as soon as they express sIgM.},
  author       = {Punnonen, J and Aversa, G and Vandekerckhove, Bart and Roncarolo, M and De Vries, J},
  issn         = {0022-1767},
  journal      = {JOURNAL OF IMMUNOLOGY},
  keywords     = {INTERLEUKIN-4,ANTIGEN,PRE-B,POKEWEED MITOGEN,MONONUCLEAR-CELLS,LYMPHOCYTES,STAPHYLOCOCCUS-AUREUS,ACUTE LYMPHOBLASTIC-LEUKEMIA,INVITRO,PRECURSORS},
  language     = {eng},
  number       = {11},
  pages        = {3398--3404},
  title        = {Induction of isotype switching and IG production by CD5+ and CD10+ human fetal B-cells},
  volume       = {148},
  year         = {1992},
}