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Characterization of a putative endoxylanase in the migratory plant-parasitic nematode Radopholus similis

Annelies Haegeman (UGent) , Bartel Vanholme (UGent) and Godelieve Gheysen (UGent)
(2009) MOLECULAR PLANT PATHOLOGY. 10(3). p.389-401
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Abstract
Plant-parasitic nematodes have developed an arsenal of enzymes to degrade the rigid plant cell wall. In this article we report the presence of a putative endoxylanase in the migratory endoparasitic nematode Radopholus similis. This enzyme is thought to facilitate the migration of the nematode, as it breaks down xylan, the major component of hemicellulose. The corresponding gene (Rs-xyl1) was cloned and the sequence revealed three small introns. Interestingly the position of all three introns was conserved in a putative endoxylanase from Meloidogyne hapla and one of them in two endoxylanases of M. incognita, which suggests a common ancestral gene. Spatial and temporal expression of the Rs-xyl1 gene was examined by in situ hybridization and semi-quantitative RT-PCR. The putative protein consists of a signal peptide, a catalytic domain and a carbohydrate binding module (CBM). The catalytic domain showed similarity to both glycosyl hydrolase family 5 (GHF5) and GHF30 enzymes. Using Hidden Markov Model profiles and phylogenetic analysis, we were able to show that Rs-XYL1 and its closest homologues are not members of GHF5 as previously suggested, but rather form a subclass within GHF30. Silencing the putative endoxylanase by dsRNA targeting the CBM region resulted in an average decrease in infection of 60%, indicating that the gene is important for the nematode to complete its lifecycle.
Keywords
ESOPHAGEAL-GLAND, CLONING, RNA INTERFERENCE, MOLECULAR-BIOLOGY, HETERODERA-GLYCINES, MICROBIAL XYLANASES, ERWINIA-CHRYSANTHEMI, PECTATE LYASE, BURSAPHELENCHUS-XYLOPHILUS, MELOIDOGYNE-INCOGNITA

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Citation

Please use this url to cite or link to this publication:

Chicago
Haegeman, Annelies, Bartel Vanholme, and Godelieve Gheysen. 2009. “Characterization of a Putative Endoxylanase in the Migratory Plant-parasitic Nematode Radopholus Similis.” Molecular Plant Pathology 10 (3): 389–401.
APA
Haegeman, Annelies, Vanholme, B., & Gheysen, G. (2009). Characterization of a putative endoxylanase in the migratory plant-parasitic nematode Radopholus similis. MOLECULAR PLANT PATHOLOGY, 10(3), 389–401.
Vancouver
1.
Haegeman A, Vanholme B, Gheysen G. Characterization of a putative endoxylanase in the migratory plant-parasitic nematode Radopholus similis. MOLECULAR PLANT PATHOLOGY. 2009;10(3):389–401.
MLA
Haegeman, Annelies, Bartel Vanholme, and Godelieve Gheysen. “Characterization of a Putative Endoxylanase in the Migratory Plant-parasitic Nematode Radopholus Similis.” MOLECULAR PLANT PATHOLOGY 10.3 (2009): 389–401. Print.
@article{623212,
  abstract     = {Plant-parasitic nematodes have developed an arsenal of enzymes to degrade the rigid plant cell wall. In this article we report the presence of a putative endoxylanase in the migratory endoparasitic nematode Radopholus similis. This enzyme is thought to facilitate the migration of the nematode, as it breaks down xylan, the major component of hemicellulose. The corresponding gene (Rs-xyl1) was cloned and the sequence revealed three small introns. Interestingly the position of all three introns was conserved in a putative endoxylanase from Meloidogyne hapla and one of them in two endoxylanases of M. incognita, which suggests a common ancestral gene. Spatial and temporal expression of the Rs-xyl1 gene was examined by in situ hybridization and semi-quantitative RT-PCR. The putative protein consists of a signal peptide, a catalytic domain and a carbohydrate binding module (CBM). The catalytic domain showed similarity to both glycosyl hydrolase family 5 (GHF5) and GHF30 enzymes. Using Hidden Markov Model profiles and phylogenetic analysis, we were able to show that Rs-XYL1 and its closest homologues are not members of GHF5 as previously suggested, but rather form a subclass within GHF30. Silencing the putative endoxylanase by dsRNA targeting the CBM region resulted in an average decrease in infection of 60\%, indicating that the gene is important for the nematode to complete its lifecycle.},
  author       = {Haegeman, Annelies and Vanholme, Bartel and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keyword      = {ESOPHAGEAL-GLAND,CLONING,RNA INTERFERENCE,MOLECULAR-BIOLOGY,HETERODERA-GLYCINES,MICROBIAL XYLANASES,ERWINIA-CHRYSANTHEMI,PECTATE LYASE,BURSAPHELENCHUS-XYLOPHILUS,MELOIDOGYNE-INCOGNITA},
  language     = {eng},
  number       = {3},
  pages        = {389--401},
  title        = {Characterization of a putative endoxylanase in the migratory plant-parasitic nematode Radopholus similis},
  url          = {http://dx.doi.org/10.1111/J.1364-3703.2009.00539.X},
  volume       = {10},
  year         = {2009},
}

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