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Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC

Steven Van der Plas (UGent) , Els Van Hoeck (UGent) , Frederic Lynen (UGent) , Patrick Sandra (UGent) and Annemieke Madder (UGent)
(2009) EUROPEAN JOURNAL OF ORGANIC CHEMISTRY. 11(Sp. Iss. SI). p.1796-1805
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Abstract
A series of human estrogen receptor (hER) mimics were synthesised. On the basis of the knowledge on the structure of the hormone binding domain of the hER, three different peptide chains were constructed onto a tripodal scaffold. By using a fully automated solid-phase synthesis protocol, 120 members with known identity were synthesised in only a week. Affinity towards estrogenic compounds was checked by affinity LC. For this purpose, ethinylestradiol was "clicked" onto a modified-silica phase, and the obtained material was packed into an HPLC column. The results stemming from the affinity LC experiments were confirmed by clicking one library member to silica and by using this solid phase to extract different endocrine-disrupting chemicals (EDCs) from aqueous media.

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Chicago
Van der Plas, Steven, Els Van Hoeck, Frederic Lynen, Patrick Sandra, and Annemieke Madder. 2009. “Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC.” European Journal of Organic Chemistry 11 (Sp. Iss. SI): 1796–1805.
APA
Van der Plas, S., Van Hoeck, E., Lynen, F., Sandra, P., & Madder, A. (2009). Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC. EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 11(Sp. Iss. SI), 1796–1805.
Vancouver
1.
Van der Plas S, Van Hoeck E, Lynen F, Sandra P, Madder A. Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC. EUROPEAN JOURNAL OF ORGANIC CHEMISTRY. 2009;11(Sp. Iss. SI):1796–805.
MLA
Van der Plas, Steven et al. “Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC.” EUROPEAN JOURNAL OF ORGANIC CHEMISTRY 11.Sp. Iss. SI (2009): 1796–1805. Print.
@article{611868,
  abstract     = {A series of human estrogen receptor (hER) mimics were synthesised. On the basis of the knowledge on the structure of the hormone binding domain of the hER, three different peptide chains were constructed onto a tripodal scaffold. By using a fully automated solid-phase synthesis protocol, 120 members with known identity were synthesised in only a week. Affinity towards estrogenic compounds was checked by affinity LC. For this purpose, ethinylestradiol was {\textacutedbl}clicked{\textacutedbl} onto a modified-silica phase, and the obtained material was packed into an HPLC column. The results stemming from the affinity LC experiments were confirmed by clicking one library member to silica and by using this solid phase to extract different endocrine-disrupting chemicals (EDCs) from aqueous media.},
  author       = {Van der Plas, Steven and Van Hoeck, Els and Lynen, Frederic and Sandra, Patrick and Madder, Annemieke},
  issn         = {1434-193X},
  journal      = {EUROPEAN JOURNAL OF ORGANIC CHEMISTRY},
  language     = {eng},
  number       = {Sp. Iss. SI},
  pages        = {1796--1805},
  title        = {Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC},
  url          = {http://dx.doi.org/10.1002/ejoc.200801290},
  volume       = {11},
  year         = {2009},
}

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