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Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins after Polyacrylamide Gel Electrophoresis

Zhenzhen Wang, Xia Liu, Willy Baeyens (UGent) , Joris Delanghe (UGent) and Jin Ouyang
(2008) JOURNAL OF PROTEOME RESEARCH. 7(12). p.5075-5081
Author
Organization
Abstract
A novel chemiluminescent probe, copper(II)-Alizarin Red S (ARS) complex, for the detection of human serum proteins after polyacrylamide gel electrophoresis (PAGE) is described. The detection is based on the binding of the copper(II)-ARS complex to proteins and the catalytic activity of copper(II) in the luminol-hydrogen peroxide chemiluminescence (CL) system. Various proteins are directly detected in polyacrylamide gels, avoiding tedious transferring procedures. In the present study, the possible reaction mechanism and sensitivity evaluation are analyzed. The experimental conditions such as solution concentration, complex ratio, and washing reagents are likewise optimized. The proposed method offers simple, fast, and sensitive detection of serum proteins. As a novel chemiluminescent detection method, it shows significant analytical potential in biochemistry.
Keywords
Chemiluminescence imaging, Copper(II)-Alizarin Red S complex, Probe, Human serum proteins, CBB staining, IMAGING DETECTION, SYPRO ORANGE, CHEMI-LUMINESCENCE I, BINDING, MEMBRANES, IRON, ALBUMIN, SILVER, STAINS

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Chicago
Wang, Zhenzhen, Xia Liu, Willy Baeyens, Joris Delanghe, and Jin Ouyang. 2008. “Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins After Polyacrylamide Gel Electrophoresis.” Journal of Proteome Research 7 (12): 5075–5081.
APA
Wang, Zhenzhen, Liu, X., Baeyens, W., Delanghe, J., & Ouyang, J. (2008). Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins after Polyacrylamide Gel Electrophoresis. JOURNAL OF PROTEOME RESEARCH, 7(12), 5075–5081.
Vancouver
1.
Wang Z, Liu X, Baeyens W, Delanghe J, Ouyang J. Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins after Polyacrylamide Gel Electrophoresis. JOURNAL OF PROTEOME RESEARCH. 1155 16TH ST, NW, WASHINGTON, DC 20036 USA: AMER CHEMICAL SOC; 2008;7(12):5075–81.
MLA
Wang, Zhenzhen, Xia Liu, Willy Baeyens, et al. “Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins After Polyacrylamide Gel Electrophoresis.” JOURNAL OF PROTEOME RESEARCH 7.12 (2008): 5075–5081. Print.
@article{606702,
  abstract     = {A novel chemiluminescent probe, copper(II)-Alizarin Red S (ARS) complex, for the detection of human serum proteins after polyacrylamide gel electrophoresis (PAGE) is described. The detection is based on the binding of the copper(II)-ARS complex to proteins and the catalytic activity of copper(II) in the luminol-hydrogen peroxide chemiluminescence (CL) system. Various proteins are directly detected in polyacrylamide gels, avoiding tedious transferring procedures. In the present study, the possible reaction mechanism and sensitivity evaluation are analyzed. The experimental conditions such as solution concentration, complex ratio, and washing reagents are likewise optimized. The proposed method offers simple, fast, and sensitive detection of serum proteins. As a novel chemiluminescent detection method, it shows significant analytical potential in biochemistry.},
  author       = {Wang, Zhenzhen and Liu, Xia and Baeyens, Willy and Delanghe, Joris and Ouyang, Jin},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {Chemiluminescence imaging,Copper(II)-Alizarin Red S complex,Probe,Human serum proteins,CBB staining,IMAGING DETECTION,SYPRO ORANGE,CHEMI-LUMINESCENCE I,BINDING,MEMBRANES,IRON,ALBUMIN,SILVER,STAINS},
  language     = {eng},
  number       = {12},
  pages        = {5075--5081},
  publisher    = {AMER CHEMICAL SOC},
  title        = {Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins after Polyacrylamide Gel Electrophoresis},
  url          = {http://dx.doi.org/10.1021/pr800365n},
  volume       = {7},
  year         = {2008},
}
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