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Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes

Ine Lentacker (UGent) , Nan Wang (UGent) , Roosmarijn Vandenbroucke (UGent) , Jo Demeester (UGent) , Stefaan De Smedt (UGent) and Niek Sanders (UGent)
(2009) MOLECULAR PHARMACEUTICS. 6(2). p.457-467
Author
Organization
Abstract
Recently we reported that the transfection of cells by PEGylated lipoplexes becomes significantly better by binding the PEGylated lipoplexes to the surface of microbubbles and applying ultrasound. To further optimize this gene delivery system it is important to understand the working mechanism. This paper elucidates the cellular entry path of these lipoplexes. The results clearly show that the PEGylated lipoplexes, released from the microbubbles upon applying ultrasound, are not taken up by endocytosis, the most common route for nanoparticles to enter cells. Our data demonstrate that, upon implosion of the microbubbles, the PEGylated lipoplexes are released and are most probably able to passively diffuse through the cell membrane pores or become injected in the cytoplasm of the target cells. This is attractive as the in vivo use of PEGylated nanoparticles remains currently limited due to a decreased cellular uptake and inefficient escape of the PEGylated nanoparticles from the endosomes.
Keywords
Ultrasound, microbubbles, intracellular pathway, PEGylated lipoplexes, microjets, GENE DELIVERY, INTRACELLULAR DELIVERY, MEDIATED TRANSFECTION, IN-VITRO, CELL INTERACTION, ENHANCED ULTRASOUND, PHOTOCHEMICAL INTERNALIZATION, CONTRAST AGENTS, PLASMID DNA, CAVITATION

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Chicago
Lentacker, Ine, Nan Wang, Roosmarijn Vandenbroucke, Jo Demeester, Stefaan De Smedt, and Niek Sanders. 2009. “Ultrasound Exposure of Lipoplex Loaded Microbubbles Facilitates Direct Cytoplasmic Entry of the Lipoplexes.” Molecular Pharmaceutics 6 (2): 457–467.
APA
Lentacker, I., Wang, N., Vandenbroucke, R., Demeester, J., De Smedt, S., & Sanders, N. (2009). Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes. MOLECULAR PHARMACEUTICS, 6(2), 457–467.
Vancouver
1.
Lentacker I, Wang N, Vandenbroucke R, Demeester J, De Smedt S, Sanders N. Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes. MOLECULAR PHARMACEUTICS. 2009;6(2):457–67.
MLA
Lentacker, Ine, Nan Wang, Roosmarijn Vandenbroucke, et al. “Ultrasound Exposure of Lipoplex Loaded Microbubbles Facilitates Direct Cytoplasmic Entry of the Lipoplexes.” MOLECULAR PHARMACEUTICS 6.2 (2009): 457–467. Print.
@article{603918,
  abstract     = {Recently we reported that the transfection of cells by PEGylated lipoplexes becomes significantly better by binding the PEGylated lipoplexes to the surface of microbubbles and applying ultrasound. To further optimize this gene delivery system it is important to understand the working mechanism. This paper elucidates the cellular entry path of these lipoplexes. The results clearly show that the PEGylated lipoplexes, released from the microbubbles upon applying ultrasound, are not taken up by endocytosis, the most common route for nanoparticles to enter cells. Our data demonstrate that, upon implosion of the microbubbles, the PEGylated lipoplexes are released and are most probably able to passively diffuse through the cell membrane pores or become injected in the cytoplasm of the target cells. This is attractive as the in vivo use of PEGylated nanoparticles remains currently limited due to a decreased cellular uptake and inefficient escape of the PEGylated nanoparticles from the endosomes.},
  author       = {Lentacker, Ine and Wang, Nan and Vandenbroucke, Roosmarijn and Demeester, Jo and De Smedt, Stefaan and Sanders, Niek},
  issn         = {1543-8384},
  journal      = {MOLECULAR PHARMACEUTICS},
  language     = {eng},
  number       = {2},
  pages        = {457--467},
  title        = {Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes},
  url          = {http://dx.doi.org/10.1021/mp800154s},
  volume       = {6},
  year         = {2009},
}

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