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Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes

Ine Lentacker UGent, Nan Wang UGent, Roosmarijn Vandenbroucke UGent, Jo Demeester UGent, Stefaan De Smedt UGent and Niek Sanders UGent (2009) MOLECULAR PHARMACEUTICS. 6(2). p.457-467
abstract
Recently we reported that the transfection of cells by PEGylated lipoplexes becomes significantly better by binding the PEGylated lipoplexes to the surface of microbubbles and applying ultrasound. To further optimize this gene delivery system it is important to understand the working mechanism. This paper elucidates the cellular entry path of these lipoplexes. The results clearly show that the PEGylated lipoplexes, released from the microbubbles upon applying ultrasound, are not taken up by endocytosis, the most common route for nanoparticles to enter cells. Our data demonstrate that, upon implosion of the microbubbles, the PEGylated lipoplexes are released and are most probably able to passively diffuse through the cell membrane pores or become injected in the cytoplasm of the target cells. This is attractive as the in vivo use of PEGylated nanoparticles remains currently limited due to a decreased cellular uptake and inefficient escape of the PEGylated nanoparticles from the endosomes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Ultrasound, microbubbles, intracellular pathway, PEGylated lipoplexes, microjets, GENE DELIVERY, INTRACELLULAR DELIVERY, MEDIATED TRANSFECTION, IN-VITRO, CELL INTERACTION, ENHANCED ULTRASOUND, PHOTOCHEMICAL INTERNALIZATION, CONTRAST AGENTS, PLASMID DNA, CAVITATION
journal title
MOLECULAR PHARMACEUTICS
Mol. Pharm.
volume
6
issue
2
pages
457 - 467
Web of Science type
Article
Web of Science id
000264992400013
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
5.408 (2009)
JCR rank
16/236 (2009)
JCR quartile
1 (2009)
ISSN
1543-8384
DOI
10.1021/mp800154s
language
English
UGent publication?
yes
classification
A1
id
603918
handle
http://hdl.handle.net/1854/LU-603918
date created
2009-04-29 15:43:54
date last changed
2009-10-08 13:48:26
@article{603918,
  abstract     = {Recently we reported that the transfection of cells by PEGylated lipoplexes becomes significantly better by binding the PEGylated lipoplexes to the surface of microbubbles and applying ultrasound. To further optimize this gene delivery system it is important to understand the working mechanism. This paper elucidates the cellular entry path of these lipoplexes. The results clearly show that the PEGylated lipoplexes, released from the microbubbles upon applying ultrasound, are not taken up by endocytosis, the most common route for nanoparticles to enter cells. Our data demonstrate that, upon implosion of the microbubbles, the PEGylated lipoplexes are released and are most probably able to passively diffuse through the cell membrane pores or become injected in the cytoplasm of the target cells. This is attractive as the in vivo use of PEGylated nanoparticles remains currently limited due to a decreased cellular uptake and inefficient escape of the PEGylated nanoparticles from the endosomes.},
  author       = {Lentacker, Ine and Wang, Nan and Vandenbroucke, Roosmarijn and Demeester, Jo and De Smedt, Stefaan and Sanders, Niek},
  issn         = {1543-8384},
  journal      = {MOLECULAR PHARMACEUTICS},
  keyword      = {Ultrasound,microbubbles,intracellular pathway,PEGylated lipoplexes,microjets,GENE DELIVERY,INTRACELLULAR DELIVERY,MEDIATED TRANSFECTION,IN-VITRO,CELL INTERACTION,ENHANCED ULTRASOUND,PHOTOCHEMICAL INTERNALIZATION,CONTRAST AGENTS,PLASMID DNA,CAVITATION},
  language     = {eng},
  number       = {2},
  pages        = {457--467},
  title        = {Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes},
  url          = {http://dx.doi.org/10.1021/mp800154s},
  volume       = {6},
  year         = {2009},
}

Chicago
Lentacker, Ine, Nan Wang, Roosmarijn Vandenbroucke, Jo Demeester, Stefaan De Smedt, and Niek Sanders. 2009. “Ultrasound Exposure of Lipoplex Loaded Microbubbles Facilitates Direct Cytoplasmic Entry of the Lipoplexes.” Molecular Pharmaceutics 6 (2): 457–467.
APA
Lentacker, I., Wang, N., Vandenbroucke, R., Demeester, J., De Smedt, S., & Sanders, N. (2009). Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes. MOLECULAR PHARMACEUTICS, 6(2), 457–467.
Vancouver
1.
Lentacker I, Wang N, Vandenbroucke R, Demeester J, De Smedt S, Sanders N. Ultrasound exposure of lipoplex loaded microbubbles facilitates direct cytoplasmic entry of the lipoplexes. MOLECULAR PHARMACEUTICS. 2009;6(2):457–67.
MLA
Lentacker, Ine, Nan Wang, Roosmarijn Vandenbroucke, et al. “Ultrasound Exposure of Lipoplex Loaded Microbubbles Facilitates Direct Cytoplasmic Entry of the Lipoplexes.” MOLECULAR PHARMACEUTICS 6.2 (2009): 457–467. Print.