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Identification of protein binders in works of art by high-performance liquid chromatography-diode array detector analysis of their tryptic digests

Wim Fremout UGent, Jana Sanyova, Steven Saverwyns, Peter Vandenabeele UGent and Luc Moens UGent (2009) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 393(8). p.1991-1999
abstract
Proteins in works of art are generally determined by the relative amounts of amino acids. This method, however, implies a loss of information on the protein structure and its modifications. Consequently, we propose a method based on the analysis of trypsin digests using high-performance liquid chromatography (HPLC) UV diode array detection (DAD) for painting binder studies. All reaction steps are done in the same vial; no extraction methods or sample transfer is needed, reducing the risk of sample losses. A collection of pure binders (collagen, ovalbumin, yolk and casein) as well as homemade and historical paint samples have been investigated with this method. Chromatograms of unknowns at 214 nm and 280 nm are compared with those of the reference samples as a fingerprint. There is a good agreement between many peptides, but others seem to have been lost or their retention time shifted due to small compositional changes because of ageing and degradation of the paint. The results are comparable with the results of other techniques used for binder identification on the same samples, with the additional advantage of differentiation between egg yolk and glair.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
WALL PAINTING SAMPLES, ETHYL CHLOROFORMATE DERIVATIVES, BINDING MEDIA, MASS-SPECTROMETRY, AMINO-ACIDS, INFRARED-SPECTROSCOPY, RAMAN-SPECTROSCOPY, MICRO-RAMAN, GC-MS, PYROLYSIS, HPLC-DAD, Ancient art objects, Tryptic digest, Binding media, Cultural heritage samples, Protein binder
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
393
issue
8
pages
1991 - 1999
conference name
15th Biennial conference on Applied Surface Analysis
conference location
Soest, Germany
conference start
2008-09-08
conference end
2008-09-10
Web of Science type
Proceedings Paper
Web of Science id
000264697900021
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.48 (2009)
JCR rank
6/70 (2009)
JCR quartile
1 (2009)
ISSN
1618-2642
DOI
10.1007/s00216-009-2686-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
600763
handle
http://hdl.handle.net/1854/LU-600763
date created
2009-04-27 09:49:33
date last changed
2012-11-09 14:02:35
@article{600763,
  abstract     = {Proteins in works of art are generally determined by the relative amounts of amino acids. This method, however, implies a loss of information on the protein structure and its modifications. Consequently, we propose a method based on the analysis of trypsin digests using high-performance liquid chromatography (HPLC) UV diode array detection (DAD) for painting binder studies. All reaction steps are done in the same vial; no extraction methods or sample transfer is needed, reducing the risk of sample losses. A collection of pure binders (collagen, ovalbumin, yolk and casein) as well as homemade and historical paint samples have been investigated with this method. Chromatograms of unknowns at 214 nm and 280 nm are compared with those of the reference samples as a fingerprint. There is a good agreement between many peptides, but others seem to have been lost or their retention time shifted due to small compositional changes because of ageing and degradation of the paint. The results are comparable with the results of other techniques used for binder identification on the same samples, with the additional advantage of differentiation between egg yolk and glair.},
  author       = {Fremout, Wim and Sanyova, Jana and Saverwyns, Steven and Vandenabeele, Peter and Moens, Luc},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {WALL PAINTING SAMPLES,ETHYL CHLOROFORMATE DERIVATIVES,BINDING MEDIA,MASS-SPECTROMETRY,AMINO-ACIDS,INFRARED-SPECTROSCOPY,RAMAN-SPECTROSCOPY,MICRO-RAMAN,GC-MS,PYROLYSIS,HPLC-DAD,Ancient art objects,Tryptic digest,Binding media,Cultural heritage samples,Protein binder},
  language     = {eng},
  location     = {Soest, Germany},
  number       = {8},
  pages        = {1991--1999},
  title        = {Identification of protein binders in works of art by high-performance liquid chromatography-diode array detector analysis of their tryptic digests},
  url          = {http://dx.doi.org/10.1007/s00216-009-2686-z},
  volume       = {393},
  year         = {2009},
}

Chicago
Fremout, Wim, Jana Sanyova, Steven Saverwyns, Peter Vandenabeele, and Luc Moens. 2009. “Identification of Protein Binders in Works of Art by High-performance Liquid Chromatography-diode Array Detector Analysis of Their Tryptic Digests.” Analytical and Bioanalytical Chemistry 393 (8): 1991–1999.
APA
Fremout, W., Sanyova, J., Saverwyns, S., Vandenabeele, P., & Moens, L. (2009). Identification of protein binders in works of art by high-performance liquid chromatography-diode array detector analysis of their tryptic digests. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 393(8), 1991–1999. Presented at the 15th Biennial conference on Applied Surface Analysis.
Vancouver
1.
Fremout W, Sanyova J, Saverwyns S, Vandenabeele P, Moens L. Identification of protein binders in works of art by high-performance liquid chromatography-diode array detector analysis of their tryptic digests. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2009;393(8):1991–9.
MLA
Fremout, Wim, Jana Sanyova, Steven Saverwyns, et al. “Identification of Protein Binders in Works of Art by High-performance Liquid Chromatography-diode Array Detector Analysis of Their Tryptic Digests.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 393.8 (2009): 1991–1999. Print.