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Fluoxetine suppresses calcium signaling in human T lymphocytes through depletion of intracellular calcium stores

(2015) CELL CALCIUM. 58(3). p.254-263
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Abstract
Selective serotonin reuptake inhibitors, such as fluoxetine, have recently been shown to exert anti-inflammatory and immunosuppressive effects. Although the effects on cytokine secretion, proliferation and viability of T lymphocytes have been extensively characterized, little is known about the mechanism behind these effects. It is well known that Ca2+ signaling is an important step in the signaling transduction pathway following T cell receptor activation. Therefore, we investigated if fluoxetine interferes with Ca2+ signaling in Jurkat T lymphocytes. Fluoxetine was found to suppress Ca2+ signaling in response to T cell receptor activation. Moreover, fluoxetine was found to deplete intracellular Ca2+ stores, thereby leaving less Ca2+ available for release upon IP3- and ryanodine-receptor activation. The Ca2+-modifying effects of fluoxetine are not related to its capability to block the serotonin transporter, as even a large excess of 5HT did not abolish the effects. In conclusion, these data show that fluoxetine decreases IP3- and ryanodine-receptor mediated Ca2+ release in Jurkat T lymphocytes, an effect likely to be at the basis of the observed immunosuppression.
Keywords
SSRI, Fluoxetine, T lymphocyte, Calcium signaling, Insitol 1, 4, 5-trisphosphate, Ryanodine receptor, SEROTONIN REUPTAKE INHIBITORS, CA2+ RELEASE, PC12 CELLS, ACTIVATION, PROLIFERATION, MOBILIZATION, EXPRESSION, MACHINERY, RECEPTORS, CHANNELS

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Citation

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Chicago
Gobin, Veerle, Marijke De Bock, Bart Broeckx, Maja Kiselinova, Ward De Spiegelaere, Linos Vandekerckhove, Katleen Van Steendam, Luc Leybaert, and Dieter Deforce. 2015. “Fluoxetine Suppresses Calcium Signaling in Human T Lymphocytes Through Depletion of Intracellular Calcium Stores.” Cell Calcium 58 (3): 254–263.
APA
Gobin, V., De Bock, M., Broeckx, B., Kiselinova, M., De Spiegelaere, W., Vandekerckhove, L., Van Steendam, K., et al. (2015). Fluoxetine suppresses calcium signaling in human T lymphocytes through depletion of intracellular calcium stores. CELL CALCIUM, 58(3), 254–263.
Vancouver
1.
Gobin V, De Bock M, Broeckx B, Kiselinova M, De Spiegelaere W, Vandekerckhove L, et al. Fluoxetine suppresses calcium signaling in human T lymphocytes through depletion of intracellular calcium stores. CELL CALCIUM. 2015;58(3):254–63.
MLA
Gobin, Veerle, Marijke De Bock, Bart Broeckx, et al. “Fluoxetine Suppresses Calcium Signaling in Human T Lymphocytes Through Depletion of Intracellular Calcium Stores.” CELL CALCIUM 58.3 (2015): 254–263. Print.
@article{5991757,
  abstract     = {Selective serotonin reuptake inhibitors, such as fluoxetine, have recently been shown to exert anti-inflammatory and immunosuppressive effects. Although the effects on cytokine secretion, proliferation and viability of T lymphocytes have been extensively characterized, little is known about the mechanism behind these effects. It is well known that Ca2+ signaling is an important step in the signaling transduction pathway following T cell receptor activation. Therefore, we investigated if fluoxetine interferes with Ca2+ signaling in Jurkat T lymphocytes. Fluoxetine was found to suppress Ca2+ signaling in response to T cell receptor activation. Moreover, fluoxetine was found to deplete intracellular Ca2+ stores, thereby leaving less Ca2+ available for release upon IP3- and ryanodine-receptor activation. The Ca2+-modifying effects of fluoxetine are not related to its capability to block the serotonin transporter, as even a large excess of 5HT did not abolish the effects. In conclusion, these data show that fluoxetine decreases IP3- and ryanodine-receptor mediated Ca2+ release in Jurkat T lymphocytes, an effect likely to be at the basis of the observed immunosuppression.},
  author       = {Gobin, Veerle and De Bock, Marijke and Broeckx, Bart and Kiselinova, Maja and De Spiegelaere, Ward and Vandekerckhove, Linos and Van Steendam, Katleen and Leybaert, Luc and Deforce, Dieter},
  issn         = {0143-4160},
  journal      = {CELL CALCIUM},
  language     = {eng},
  number       = {3},
  pages        = {254--263},
  title        = {Fluoxetine suppresses calcium signaling in human T lymphocytes through depletion of intracellular calcium stores},
  url          = {http://dx.doi.org/10.1016/j.ceca.2015.06.003},
  volume       = {58},
  year         = {2015},
}

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