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Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut

Liesbeth Allais, Frederiek-Maarten Kerckhof UGent, Stephanie Verscheure, Ken Bracke UGent, Rebecca De Smet, Debby Laukens UGent, Pieter Van den Abbeele, Martine De Vos UGent, Nico Boon UGent, Guy Brusselle UGent, et al. (2016) ENVIRONMENTAL MICROBIOLOGY. 18(5). p.1352-1363
abstract
Inflammatory Bowel Diseases (IBD) are complex multifactorial diseases characterized by an inappropriate host response to an altered commensal microbiome and dysfunctional mucus barrier. Cigarette smoking is the best known environmental risk factor in IBD. Here, we studied the influence of chronic smoke exposure on the gut microbiome and mucus layer composition in conventional mice. We compared smoke-exposed to air-exposed mice (n = 12) after a smoke exposure of 24 weeks. Both Illumina sequencing (n = 6) and denaturing gradient gel electrophoresis (DGGE) (n = 12) showed that bacterial activity and community structure were significantly altered in the colon due to smoke exposure. Interestingly, an increase of Lachnospiraceae sp. activity in the colon was observed. Also, changes in the mRNA expression Muc2 and Muc3 increased in the ileum, whereas Muc4 increased in the distal colon of smoke-exposed mice (n = 6). Furthermore, we observed increased Cxcl2 and decreased Ifn-γ in the ileum, and increased Il-6 and decreased Tgf-β in the proximal colon. Tight junction gene expression remained unchanged. We infer that the modulating role of chronic smoke exposure as a latently present risk factor in the gut may be driven by the altered epithelial mucus profiles and changes in microbiome composition and immune factors.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CROHNS-DISEASE, INTESTINAL INFLAMMATION, BOWEL-DISEASE, ENVIRONMENTAL RISK-FACTORS, EPIDEMIOLOGY, 16S RIBOSOMAL-RNA, GRADIENT GEL-ELECTROPHORESIS, dysbiosis, inflammatory bowel disease, mucus layer, cigarette smoking, gut microbiome, INFECTION, EXPRESSION, MICE
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
editor
Kenneth Timmis
volume
18
issue
5
pages
1352 - 1363
Web of Science type
Article
Web of Science id
000375481200007
JCR category
MICROBIOLOGY
JCR impact factor
5.395 (2016)
JCR rank
17/124 (2016)
JCR quartile
1 (2016)
ISSN
1462-2912
DOI
10.1111/1462-2920.12934
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5990375
handle
http://hdl.handle.net/1854/LU-5990375
date created
2015-06-10 16:23:43
date last changed
2016-12-19 15:46:14
@article{5990375,
  abstract     = {Inflammatory Bowel Diseases (IBD) are complex multifactorial diseases characterized by an inappropriate host response to an altered commensal microbiome and dysfunctional mucus barrier. Cigarette smoking is the best known environmental risk factor in IBD. Here, we studied the influence of chronic smoke exposure on the gut microbiome and mucus layer composition in conventional mice. We compared smoke-exposed to air-exposed mice (n = 12) after a smoke exposure of 24 weeks. Both Illumina sequencing (n = 6) and denaturing gradient gel electrophoresis (DGGE) (n = 12) showed that bacterial activity and community structure were significantly altered in the colon due to smoke exposure. Interestingly, an increase of Lachnospiraceae sp. activity in the colon was observed. Also, changes in the mRNA expression Muc2 and Muc3 increased in the ileum, whereas Muc4 increased in the distal colon of smoke-exposed mice (n = 6). Furthermore, we observed increased Cxcl2 and decreased Ifn-\ensuremath{\gamma} in the ileum, and increased Il-6 and decreased Tgf-\ensuremath{\beta} in the proximal colon. Tight junction gene expression remained unchanged. We infer that the modulating role of chronic smoke exposure as a latently present risk factor in the gut may be driven by the altered epithelial mucus profiles and changes in microbiome composition and immune factors.},
  author       = {Allais, Liesbeth and Kerckhof, Frederiek-Maarten and Verscheure, Stephanie and Bracke, Ken and De Smet, Rebecca and Laukens, Debby and Van den Abbeele, Pieter and De Vos, Martine and Boon, Nico and Brusselle, Guy and Cuvelier, Claude and Van de Wiele, Tom},
  editor       = {Timmis, Kenneth},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {CROHNS-DISEASE,INTESTINAL INFLAMMATION,BOWEL-DISEASE,ENVIRONMENTAL RISK-FACTORS,EPIDEMIOLOGY,16S RIBOSOMAL-RNA,GRADIENT GEL-ELECTROPHORESIS,dysbiosis,inflammatory bowel disease,mucus layer,cigarette smoking,gut microbiome,INFECTION,EXPRESSION,MICE},
  language     = {eng},
  number       = {5},
  pages        = {1352--1363},
  title        = {Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut},
  url          = {http://dx.doi.org/10.1111/1462-2920.12934},
  volume       = {18},
  year         = {2016},
}

Chicago
Allais, Liesbeth, Frederiek-Maarten Kerckhof, Stephanie Verscheure, Ken Bracke, Rebecca De Smet, Debby Laukens, Pieter Van den Abbeele, et al. 2016. “Chronic Cigarette Smoke Exposure Induces Microbial and Inflammatory Shifts and Mucin Changes in the Murine Gut.” Ed. Kenneth Timmis. Environmental Microbiology 18 (5): 1352–1363.
APA
Allais, L., Kerckhof, F.-M., Verscheure, S., Bracke, K., De Smet, R., Laukens, D., Van den Abbeele, P., et al. (2016). Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut. (K. Timmis, Ed.)ENVIRONMENTAL MICROBIOLOGY, 18(5), 1352–1363.
Vancouver
1.
Allais L, Kerckhof F-M, Verscheure S, Bracke K, De Smet R, Laukens D, et al. Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut. Timmis K, editor. ENVIRONMENTAL MICROBIOLOGY. 2016;18(5):1352–63.
MLA
Allais, Liesbeth, Frederiek-Maarten Kerckhof, Stephanie Verscheure, et al. “Chronic Cigarette Smoke Exposure Induces Microbial and Inflammatory Shifts and Mucin Changes in the Murine Gut.” Ed. Kenneth Timmis. ENVIRONMENTAL MICROBIOLOGY 18.5 (2016): 1352–1363. Print.