Advanced search
1 file | 1.08 MB

Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut

Liesbeth Allais (UGent) , Frederiek-Maarten Kerckhof (UGent) , Stephanie Verscheure, Ken Bracke (UGent) , Rebecca De Smet (UGent) , Debby Laukens (UGent) , Pieter Van den Abbeele (UGent) , Martine De Vos (UGent) , Nico Boon (UGent) , Guy Brusselle (UGent) , et al.
(2016) ENVIRONMENTAL MICROBIOLOGY. 18(5). p.1352-1363
Author
Organization
Abstract
Inflammatory Bowel Diseases (IBD) are complex multifactorial diseases characterized by an inappropriate host response to an altered commensal microbiome and dysfunctional mucus barrier. Cigarette smoking is the best known environmental risk factor in IBD. Here, we studied the influence of chronic smoke exposure on the gut microbiome and mucus layer composition in conventional mice. We compared smoke-exposed to air-exposed mice (n = 12) after a smoke exposure of 24 weeks. Both Illumina sequencing (n = 6) and denaturing gradient gel electrophoresis (DGGE) (n = 12) showed that bacterial activity and community structure were significantly altered in the colon due to smoke exposure. Interestingly, an increase of Lachnospiraceae sp. activity in the colon was observed. Also, changes in the mRNA expression Muc2 and Muc3 increased in the ileum, whereas Muc4 increased in the distal colon of smoke-exposed mice (n = 6). Furthermore, we observed increased Cxcl2 and decreased Ifn-γ in the ileum, and increased Il-6 and decreased Tgf-β in the proximal colon. Tight junction gene expression remained unchanged. We infer that the modulating role of chronic smoke exposure as a latently present risk factor in the gut may be driven by the altered epithelial mucus profiles and changes in microbiome composition and immune factors.
Keywords
CROHNS-DISEASE, INTESTINAL INFLAMMATION, BOWEL-DISEASE, ENVIRONMENTAL RISK-FACTORS, EPIDEMIOLOGY, 16S RIBOSOMAL-RNA, GRADIENT GEL-ELECTROPHORESIS, dysbiosis, inflammatory bowel disease, mucus layer, cigarette smoking, gut microbiome, INFECTION, EXPRESSION, MICE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.08 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Allais, Liesbeth, Frederiek-Maarten Kerckhof, Stephanie Verscheure, Ken Bracke, Rebecca De Smet, Debby Laukens, Pieter Van den Abbeele, et al. 2016. “Chronic Cigarette Smoke Exposure Induces Microbial and Inflammatory Shifts and Mucin Changes in the Murine Gut.” Ed. Kenneth Timmis. Environmental Microbiology 18 (5): 1352–1363.
APA
Allais, L., Kerckhof, F.-M., Verscheure, S., Bracke, K., De Smet, R., Laukens, D., Van den Abbeele, P., et al. (2016). Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut. (K. Timmis, Ed.)ENVIRONMENTAL MICROBIOLOGY, 18(5), 1352–1363.
Vancouver
1.
Allais L, Kerckhof F-M, Verscheure S, Bracke K, De Smet R, Laukens D, et al. Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut. Timmis K, editor. ENVIRONMENTAL MICROBIOLOGY. 2016;18(5):1352–63.
MLA
Allais, Liesbeth, Frederiek-Maarten Kerckhof, Stephanie Verscheure, et al. “Chronic Cigarette Smoke Exposure Induces Microbial and Inflammatory Shifts and Mucin Changes in the Murine Gut.” Ed. Kenneth Timmis. ENVIRONMENTAL MICROBIOLOGY 18.5 (2016): 1352–1363. Print.
@article{5990375,
  abstract     = {Inflammatory Bowel Diseases (IBD) are complex multifactorial diseases characterized by an inappropriate host response to an altered commensal microbiome and dysfunctional mucus barrier. Cigarette smoking is the best known environmental risk factor in IBD. Here, we studied the influence of chronic smoke exposure on the gut microbiome and mucus layer composition in conventional mice. We compared smoke-exposed to air-exposed mice (n = 12) after a smoke exposure of 24 weeks. Both Illumina sequencing (n = 6) and denaturing gradient gel electrophoresis (DGGE) (n = 12) showed that bacterial activity and community structure were significantly altered in the colon due to smoke exposure. Interestingly, an increase of Lachnospiraceae sp. activity in the colon was observed. Also, changes in the mRNA expression Muc2 and Muc3 increased in the ileum, whereas Muc4 increased in the distal colon of smoke-exposed mice (n = 6). Furthermore, we observed increased Cxcl2 and decreased Ifn-\ensuremath{\gamma} in the ileum, and increased Il-6 and decreased Tgf-\ensuremath{\beta} in the proximal colon. Tight junction gene expression remained unchanged. We infer that the modulating role of chronic smoke exposure as a latently present risk factor in the gut may be driven by the altered epithelial mucus profiles and changes in microbiome composition and immune factors.},
  author       = {Allais, Liesbeth and Kerckhof, Frederiek-Maarten and Verscheure, Stephanie and Bracke, Ken and De Smet, Rebecca and Laukens, Debby and Van den Abbeele, Pieter and De Vos, Martine and Boon, Nico and Brusselle, Guy and Cuvelier, Claude and Van de Wiele, Tom},
  editor       = {Timmis, Kenneth},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {CROHNS-DISEASE,INTESTINAL INFLAMMATION,BOWEL-DISEASE,ENVIRONMENTAL RISK-FACTORS,EPIDEMIOLOGY,16S RIBOSOMAL-RNA,GRADIENT GEL-ELECTROPHORESIS,dysbiosis,inflammatory bowel disease,mucus layer,cigarette smoking,gut microbiome,INFECTION,EXPRESSION,MICE},
  language     = {eng},
  number       = {5},
  pages        = {1352--1363},
  title        = {Chronic cigarette smoke exposure induces microbial and inflammatory shifts and mucin changes in the murine gut},
  url          = {http://dx.doi.org/10.1111/1462-2920.12934},
  volume       = {18},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: