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A doubling of microphytobenthos biomass coincides with a tenfold increase in denitrifier and total bacterial abundances in intertidal sediments of a temperate estuary

Helen Decleyre (UGent) , Kim Heylen (UGent) , Koen Sabbe (UGent) , Bjorn Tytgat (UGent) , Dieter Deforce (UGent) , Filip Van Nieuwerburgh (UGent) , Carl Van Colen (UGent) and Anne Willems (UGent)
(2015) PLOS ONE. 10(5).
Author
Organization
Abstract
Surface sediments are important systems for the removal of anthropogenically derived inorganic nitrogen in estuaries. They are often characterized by the presence of a microphytobenthos (MPB) biofilm, which can impact bacterial communities in underlying sediments for example by secretion of extracellular polymeric substances (EPS) and competition for nutrients (including nitrogen). Pyrosequencing and qPCR was performed on two intertidal surface sediments of the Westerschelde estuary characterized by a two-fold difference in MPB biomass but no difference in MPB composition. Doubling of MPB biomass was accompanied by a disproportionately (ten-fold) increase in total bacterial abundances while, unexpectedly, no difference in general community structure was observed, despite significantly lower bacterial richness and distinct community membership, mostly for non-abundant taxa. Denitrifier abundances corresponded likewise while community structure, both for nirS and nirK denitrifiers, remained unchanged, suggesting that competition with diatoms for nitrate is negligible at concentrations in the investigated sediments (appr. 1 mg/l NO3-). This study indicates that MPB biomass increase has a general, significantly positive effect on total bacterial and denitrifier abundances, with stimulation or inhibition of specific bacterial groups that however do not result in a re-structured community.
Keywords
estuarine sediments, denitrification, diatoms, pyrosequencing, qPCR, REDUCTASE GENES NIRS, NITROGEN-CYCLE, COMMUNITY COMPOSITION, CULTIVATED DENITRIFIERS, MICROBIAL DIVERSITY, NITRATE REDUCTION, MARINE ECOSYSTEMS, ACTIVATED-SLUDGE, UNITED-KINGDOM, RARE BIOSPHERE

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MLA
Decleyre, Helen et al. “A Doubling of Microphytobenthos Biomass Coincides with a Tenfold Increase in Denitrifier and Total Bacterial Abundances in Intertidal Sediments of a Temperate Estuary.” PLOS ONE 10.5 (2015): n. pag. Print.
APA
Decleyre, H., Heylen, K., Sabbe, K., Tytgat, B., Deforce, D., Van Nieuwerburgh, F., Van Colen, C., et al. (2015). A doubling of microphytobenthos biomass coincides with a tenfold increase in denitrifier and total bacterial abundances in intertidal sediments of a temperate estuary. PLOS ONE, 10(5).
Chicago author-date
Decleyre, Helen, Kim Heylen, Koen Sabbe, Bjorn Tytgat, Dieter Deforce, Filip Van Nieuwerburgh, Carl Van Colen, and Anne Willems. 2015. “A Doubling of Microphytobenthos Biomass Coincides with a Tenfold Increase in Denitrifier and Total Bacterial Abundances in Intertidal Sediments of a Temperate Estuary.” Plos One 10 (5).
Chicago author-date (all authors)
Decleyre, Helen, Kim Heylen, Koen Sabbe, Bjorn Tytgat, Dieter Deforce, Filip Van Nieuwerburgh, Carl Van Colen, and Anne Willems. 2015. “A Doubling of Microphytobenthos Biomass Coincides with a Tenfold Increase in Denitrifier and Total Bacterial Abundances in Intertidal Sediments of a Temperate Estuary.” Plos One 10 (5).
Vancouver
1.
Decleyre H, Heylen K, Sabbe K, Tytgat B, Deforce D, Van Nieuwerburgh F, et al. A doubling of microphytobenthos biomass coincides with a tenfold increase in denitrifier and total bacterial abundances in intertidal sediments of a temperate estuary. PLOS ONE. 2015;10(5).
IEEE
[1]
H. Decleyre et al., “A doubling of microphytobenthos biomass coincides with a tenfold increase in denitrifier and total bacterial abundances in intertidal sediments of a temperate estuary,” PLOS ONE, vol. 10, no. 5, 2015.
@article{5990267,
  abstract     = {Surface sediments are important systems for the removal of anthropogenically derived inorganic nitrogen in estuaries. They are often characterized by the presence of a microphytobenthos (MPB) biofilm, which can impact bacterial communities in underlying sediments for example by secretion of extracellular polymeric substances (EPS) and competition for nutrients (including nitrogen). Pyrosequencing and qPCR was performed on two intertidal surface sediments of the Westerschelde estuary characterized by a two-fold difference in MPB biomass but no difference in MPB composition. Doubling of MPB biomass was accompanied by a disproportionately (ten-fold) increase in total bacterial abundances while, unexpectedly, no difference in general community structure was observed, despite significantly lower bacterial richness and distinct community membership, mostly for non-abundant taxa. Denitrifier abundances corresponded likewise while community structure, both for nirS and nirK denitrifiers, remained unchanged, suggesting that competition with diatoms for nitrate is negligible at concentrations in the investigated sediments (appr. 1 mg/l NO3-). This study indicates that MPB biomass increase has a general, significantly positive effect on total bacterial and denitrifier abundances, with stimulation or inhibition of specific bacterial groups that however do not result in a re-structured community.},
  articleno    = {e0126583},
  author       = {Decleyre, Helen and Heylen, Kim and Sabbe, Koen and Tytgat, Bjorn and Deforce, Dieter and Van Nieuwerburgh, Filip and Van Colen, Carl and Willems, Anne},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {estuarine sediments,denitrification,diatoms,pyrosequencing,qPCR,REDUCTASE GENES NIRS,NITROGEN-CYCLE,COMMUNITY COMPOSITION,CULTIVATED DENITRIFIERS,MICROBIAL DIVERSITY,NITRATE REDUCTION,MARINE ECOSYSTEMS,ACTIVATED-SLUDGE,UNITED-KINGDOM,RARE BIOSPHERE},
  language     = {eng},
  number       = {5},
  pages        = {23},
  title        = {A doubling of microphytobenthos biomass coincides with a tenfold increase in denitrifier and total bacterial abundances in intertidal sediments of a temperate estuary},
  url          = {http://dx.doi.org/10.1371/journal.pone.0126583},
  volume       = {10},
  year         = {2015},
}

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