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Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production

Maarten Ameye (UGent) , Kris Audenaert (UGent) , Nathalie De Zutter (UGent) , Kathy Steppe (UGent) , Lieven Van Meulebroek (UGent) , Lynn Vanhaecke (UGent) , David De Vleesschauwer (UGent) , Geert Haesaert (UGent) and Guy Smagghe (UGent)
(2015) PLANT PHYSIOLOGY. 167(4). p.1671-1684
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Abstract
Priming refers to a mechanism whereby plants are sensitized to respond faster and/or more strongly to future pathogen attack. Here, we demonstrate that preexposure to the green leaf volatile Z-3-hexenyl acetate (Z-3-HAC) primed wheat (Triticum aestivum) for enhanced defense against subsequent infection with the hemibiotrophic fungus Fusarium graminearum. Bioassays showed that, after priming with Z-3-HAC, wheat ears accumulated up to 40% fewer necrotic spikelets. Furthermore, leaves of seedlings showed significantly smaller necrotic lesions comparedwith nonprimed plants, coinciding with strongly reduced fungal growth in planta. Additionally, we found that F. graminearum produced more deoxynivalenol, a mycotoxin, in the primed treatment. Expression analysis of salicylic acid (SA) and jasmonic acid (JA) biosynthesis genes and exogenous methyl salicylate and methyl jasmonate applications showed that plant defense against F. graminearum is sequentially regulated by SA and JA during the early and later stages of infection, respectively. Interestingly, analysis of the effect of Z-3-HAC pretreatment on SA-and JA-responsive gene expression in hormone-treated and pathogen-inoculated seedlings revealed that Z-3-HAC boosts JA-dependent defenses during the necrotrophic infection stage of F. graminearum but suppresses SA-regulated defense during its biotrophic phase. Together, these findings highlight the importance of temporally separated hormone changes in molding plant health and disease and support a scenario whereby the green leaf volatile Z-3-HAC protects wheat against Fusarium head blight by priming for enhanced JA-dependent defenses during the necrotrophic stages of infection.
Keywords
SMALL-GRAIN CEREALS, PROGRAMMED CELL-DEATH, PLANT VOLATILES, HEAD BLIGHT, HEXANOIC ACID, ALTERNARIA-ALTERNATA, INDUCED RESISTANCE, BOTRYTIS-CINEREA, GENE-EXPRESSION, SALICYLIC-ACID

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Chicago
Ameye, Maarten, Kris Audenaert, Nathalie De Zutter, Kathy Steppe, Lieven Van Meulebroek, Lynn Vanhaecke, David De Vleesschauwer, Geert Haesaert, and Guy Smagghe. 2015. “Priming of Wheat with the Green Leaf Volatile Z-3-hexenyl Acetate Enhances Defense Against Fusarium Graminearum but Boosts Deoxynivalenol Production.” Plant Physiology 167 (4): 1671–1684.
APA
Ameye, M., Audenaert, K., De Zutter, N., Steppe, K., Van Meulebroek, L., Vanhaecke, L., De Vleesschauwer, D., et al. (2015). Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production. PLANT PHYSIOLOGY, 167(4), 1671–1684.
Vancouver
1.
Ameye M, Audenaert K, De Zutter N, Steppe K, Van Meulebroek L, Vanhaecke L, et al. Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production. PLANT PHYSIOLOGY. 2015;167(4):1671–84.
MLA
Ameye, Maarten, Kris Audenaert, Nathalie De Zutter, et al. “Priming of Wheat with the Green Leaf Volatile Z-3-hexenyl Acetate Enhances Defense Against Fusarium Graminearum but Boosts Deoxynivalenol Production.” PLANT PHYSIOLOGY 167.4 (2015): 1671–1684. Print.
@article{5987735,
  abstract     = {Priming refers to a mechanism whereby plants are sensitized to respond faster and/or more strongly to future pathogen attack. Here, we demonstrate that preexposure to the green leaf volatile Z-3-hexenyl acetate (Z-3-HAC) primed wheat (Triticum aestivum) for enhanced defense against subsequent infection with the hemibiotrophic fungus Fusarium graminearum. Bioassays showed that, after priming with Z-3-HAC, wheat ears accumulated up to 40\% fewer necrotic spikelets. Furthermore, leaves of seedlings showed significantly smaller necrotic lesions comparedwith nonprimed plants, coinciding with strongly reduced fungal growth in planta. Additionally, we found that F. graminearum produced more deoxynivalenol, a mycotoxin, in the primed treatment. Expression analysis of salicylic acid (SA) and jasmonic acid (JA) biosynthesis genes and exogenous methyl salicylate and methyl jasmonate applications showed that plant defense against F. graminearum is sequentially regulated by SA and JA during the early and later stages of infection, respectively. Interestingly, analysis of the effect of Z-3-HAC pretreatment on SA-and JA-responsive gene expression in hormone-treated and pathogen-inoculated seedlings revealed that Z-3-HAC boosts JA-dependent defenses during the necrotrophic infection stage of F. graminearum but suppresses SA-regulated defense during its biotrophic phase. Together, these findings highlight the importance of temporally separated hormone changes in molding plant health and disease and support a scenario whereby the green leaf volatile Z-3-HAC protects wheat against Fusarium head blight by priming for enhanced JA-dependent defenses during the necrotrophic stages of infection.},
  author       = {Ameye, Maarten and Audenaert, Kris and De Zutter, Nathalie and Steppe, Kathy and Van Meulebroek, Lieven and Vanhaecke, Lynn and De Vleesschauwer, David and Haesaert, Geert and Smagghe, Guy},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {SMALL-GRAIN CEREALS,PROGRAMMED CELL-DEATH,PLANT VOLATILES,HEAD BLIGHT,HEXANOIC ACID,ALTERNARIA-ALTERNATA,INDUCED RESISTANCE,BOTRYTIS-CINEREA,GENE-EXPRESSION,SALICYLIC-ACID},
  language     = {eng},
  number       = {4},
  pages        = {1671--1684},
  title        = {Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production},
  url          = {http://dx.doi.org/10.1104/pp.15.00107},
  volume       = {167},
  year         = {2015},
}

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