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Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov.

Jessy Praet (UGent) , Ivan Meeus (UGent) , Margo Cnockaert (UGent) , Kurt Houf (UGent) , Guy Smagghe (UGent) and Peter Vandamme (UGent)
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Abstract
Twelve isolates of lactic acid bacteria (LAB) were obtained in the course of a bumble bee gut microbiota study and grouped into four matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry clusters. Comparative 16S rRNA gene sequence analysis revealed that cluster 1 isolates, represented by strain LMG 28288(T), are most closely related to Lactobacillus apis (97.0 % sequence similarity to that of L. apis LMG 26964(T)). Cluster 2 isolates represented by strain LMG 28290(T) are most closely related to Weissella hellenica (99.6 % sequence similarity to that of W. hellenica LMG 15125(T)). The single cluster 3 and 4 isolates had identical 16S rRNA gene sequences which were 94.8 % similar to that of Leuconostoc mesenteroides subsp. mesenteroides LMG 6893(T), their nearest phylogenetic neighbour. A polyphasic taxonomic study additionally including comparative pheS sequence analysis, DNA-DNA hybridization experiments, DNA G+C content analysis, (GTG)(5)-PCR fingerprinting and a biochemical characterization, demonstrated that cluster 1 isolates represent a novel Lactobacillus species for which we propose the name Lactobacillus bombicola sp. nov. with LMG 28288(T) (= DSM 28793(T)) as the type strain; and that cluster 2 isolates represent a novel Weissella species for which we propose the name Weissella bombi sp. nov. with LMG 28290(T) (= DSM 28794(T)) as the type strain. Cluster 3 and 4 isolates, in contrast, represented a very distinct, novel taxon that could be distinguished from members of the genera Leuconostoc and Fructobacillus, its nearest phylogenetic neighbours, by its cellular morphology, non-fructophilic metabolism and DNA G+C content. We therefore classify both isolates into a novel species representing a novel LAB genus for which the name Convivina intestini gen. nov., sp. nov. is proposed with LMG 28291(T) (= DSM 28795(T)) as the type strain.
Keywords
Lactobacillus, Lactic acid bacteria, Weissella, Leuconostocaceae, Bumble bee, Gut microbiota, HONEYBEE APIS-MELLIFERA, DIGESTIVE-TRACT, DECLINE, IDENTIFICATION, FERMENTATION, LEUCONOSTOC, VULNERABILITY, STOMACH

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Chicago
Praet, Jessy, Ivan Meeus, Margo Cnockaert, Kurt Houf, Guy Smagghe, and Peter Vandamme. 2015. “Novel Lactic Acid Bacteria Isolated from the Bumble Bee Gut: Convivina Intestini Gen. Nov., Sp. Nov., Lactobacillus Bombicola Sp. Nov., and Weissella Bombi Sp. Nov.” Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 107 (5): 1337–1349.
APA
Praet, Jessy, Meeus, I., Cnockaert, M., Houf, K., Smagghe, G., & Vandamme, P. (2015). Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 107(5), 1337–1349.
Vancouver
1.
Praet J, Meeus I, Cnockaert M, Houf K, Smagghe G, Vandamme P. Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. 2015;107(5):1337–49.
MLA
Praet, Jessy, Ivan Meeus, Margo Cnockaert, et al. “Novel Lactic Acid Bacteria Isolated from the Bumble Bee Gut: Convivina Intestini Gen. Nov., Sp. Nov., Lactobacillus Bombicola Sp. Nov., and Weissella Bombi Sp. Nov.” ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 107.5 (2015): 1337–1349. Print.
@article{5987538,
  abstract     = {Twelve isolates of lactic acid bacteria (LAB) were obtained in the course of a bumble bee gut microbiota study and grouped into four matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry clusters. Comparative 16S rRNA gene sequence analysis revealed that cluster 1 isolates, represented by strain LMG 28288(T), are most closely related to Lactobacillus apis (97.0 \% sequence similarity to that of L. apis LMG 26964(T)). Cluster 2 isolates represented by strain LMG 28290(T) are most closely related to Weissella hellenica (99.6 \% sequence similarity to that of W. hellenica LMG 15125(T)). The single cluster 3 and 4 isolates had identical 16S rRNA gene sequences which were 94.8 \% similar to that of Leuconostoc mesenteroides subsp. mesenteroides LMG 6893(T), their nearest phylogenetic neighbour. A polyphasic taxonomic study additionally including comparative pheS sequence analysis, DNA-DNA hybridization experiments, DNA G+C content analysis, (GTG)(5)-PCR fingerprinting and a biochemical characterization, demonstrated that cluster 1 isolates represent a novel Lactobacillus species for which we propose the name Lactobacillus bombicola sp. nov. with LMG 28288(T) (= DSM 28793(T)) as the type strain; and that cluster 2 isolates represent a novel Weissella species for which we propose the name Weissella bombi sp. nov. with LMG 28290(T) (= DSM 28794(T)) as the type strain. Cluster 3 and 4 isolates, in contrast, represented a very distinct, novel taxon that could be distinguished from members of the genera Leuconostoc and Fructobacillus, its nearest phylogenetic neighbours, by its cellular morphology, non-fructophilic metabolism and DNA G+C content. We therefore classify both isolates into a novel species representing a novel LAB genus for which the name Convivina intestini gen. nov., sp. nov. is proposed with LMG 28291(T) (= DSM 28795(T)) as the type strain.},
  author       = {Praet, Jessy and Meeus, Ivan and Cnockaert, Margo and Houf, Kurt and Smagghe, Guy and Vandamme, Peter},
  issn         = {0003-6072},
  journal      = {ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {1337--1349},
  title        = {Novel lactic acid bacteria isolated from the bumble bee gut: Convivina intestini gen. nov., sp. nov., Lactobacillus bombicola sp. nov., and Weissella bombi sp. nov.},
  url          = {http://dx.doi.org/10.1007/s10482-015-0429-z},
  volume       = {107},
  year         = {2015},
}

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