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Bifidobacterium commune sp. nov. isolated from the bumble bee gut

Jessy Praet (UGent) , Ivan Meeus (UGent) , Margo Cnockaert (UGent) , Maarten Aerts (UGent) , Guy Smagghe (UGent) and Peter Vandamme (UGent)
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Abstract
Bifidobacteria were isolated from the gut of Bombus lapidarius, Bombus terrestris and Bombus hypnorum bumble bees by direct isolation on modified trypticase phytone yeast extract agar. The MALDI-TOF MS profiles of four isolates (LMG 28292(T), R-53560, R-53124, LMG 28626) were found to be identical and did not cluster with the profiles of established Bifidobacterium species. Analysis of the 16S rRNA gene sequence of strain LMG 28292(T) revealed that LMG 28292(T) is most closely related to the Bifidobacterium bohemicum type strain (96.8 %), which was also isolated from bumble bee gut specimens. The hsp60 gene of strain LMG 28292(T) shows 85.8 % sequence similarity to that of the B. bohemicum type strain. The (GTG)(5)-PCR profiles and the hsp60 sequences of all four isolates were indistinguishable; however, three different phenotypes were observed among the four isolates by means of the API 50CHL microtest system. Based on the phylogenetic, genotypic and phenotypic data, we propose to classify the four isolates within the novel species Bifidobacterium commune sp. nov., with LMG 28292(T) (= DSM 28792(T)) as the type strain.
Keywords
Bumble bee, Bifidobacteria, Gut microbiota, Isolation, HONEYBEE HEALTH, DIGESTIVE-TRACT, IDENTIFICATION, DECLINE, BOMBUS, FERMENTATION, BACTERIA, TOOL

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MLA
Praet, Jessy et al. “Bifidobacterium Commune Sp. Nov. Isolated from the Bumble Bee Gut.” ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 107.5 (2015): 1307–1313. Print.
APA
Praet, J., Meeus, I., Cnockaert, M., Aerts, M., Smagghe, G., & Vandamme, P. (2015). Bifidobacterium commune sp. nov. isolated from the bumble bee gut. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 107(5), 1307–1313.
Chicago author-date
Praet, Jessy, Ivan Meeus, Margo Cnockaert, Maarten Aerts, Guy Smagghe, and Peter Vandamme. 2015. “Bifidobacterium Commune Sp. Nov. Isolated from the Bumble Bee Gut.” Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 107 (5): 1307–1313.
Chicago author-date (all authors)
Praet, Jessy, Ivan Meeus, Margo Cnockaert, Maarten Aerts, Guy Smagghe, and Peter Vandamme. 2015. “Bifidobacterium Commune Sp. Nov. Isolated from the Bumble Bee Gut.” Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 107 (5): 1307–1313.
Vancouver
1.
Praet J, Meeus I, Cnockaert M, Aerts M, Smagghe G, Vandamme P. Bifidobacterium commune sp. nov. isolated from the bumble bee gut. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. 2015;107(5):1307–13.
IEEE
[1]
J. Praet, I. Meeus, M. Cnockaert, M. Aerts, G. Smagghe, and P. Vandamme, “Bifidobacterium commune sp. nov. isolated from the bumble bee gut,” ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, vol. 107, no. 5, pp. 1307–1313, 2015.
@article{5987530,
  abstract     = {{Bifidobacteria were isolated from the gut of Bombus lapidarius, Bombus terrestris and Bombus hypnorum bumble bees by direct isolation on modified trypticase phytone yeast extract agar. The MALDI-TOF MS profiles of four isolates (LMG 28292(T), R-53560, R-53124, LMG 28626) were found to be identical and did not cluster with the profiles of established Bifidobacterium species. Analysis of the 16S rRNA gene sequence of strain LMG 28292(T) revealed that LMG 28292(T) is most closely related to the Bifidobacterium bohemicum type strain (96.8 %), which was also isolated from bumble bee gut specimens. The hsp60 gene of strain LMG 28292(T) shows 85.8 % sequence similarity to that of the B. bohemicum type strain. The (GTG)(5)-PCR profiles and the hsp60 sequences of all four isolates were indistinguishable; however, three different phenotypes were observed among the four isolates by means of the API 50CHL microtest system. Based on the phylogenetic, genotypic and phenotypic data, we propose to classify the four isolates within the novel species Bifidobacterium commune sp. nov., with LMG 28292(T) (= DSM 28792(T)) as the type strain.}},
  author       = {{Praet, Jessy and Meeus, Ivan and Cnockaert, Margo and Aerts, Maarten and Smagghe, Guy and Vandamme, Peter}},
  issn         = {{0003-6072}},
  journal      = {{ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY}},
  keywords     = {{Bumble bee,Bifidobacteria,Gut microbiota,Isolation,HONEYBEE HEALTH,DIGESTIVE-TRACT,IDENTIFICATION,DECLINE,BOMBUS,FERMENTATION,BACTERIA,TOOL}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1307--1313}},
  title        = {{Bifidobacterium commune sp. nov. isolated from the bumble bee gut}},
  url          = {{http://dx.doi.org/10.1007/s10482-015-0425-3}},
  volume       = {{107}},
  year         = {{2015}},
}

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