Advanced search
1 file | 742.89 KB Add to list

The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis

Author
Organization
Abstract
RNAi experiments in insects are characterized by great variability in efficiency; for instance beetles and locusts are very amenable to dsRNA-mediated gene silencing, while other insect groups, most notably lepidopterans, are more refractory to RNAi. Several factors can be forwarded that could affect the efficiency of RNAi, such as the composition and function of the intracellular RNAi machinery, the mechanism of dsRNA uptake, the presence of dsRNA- and siRNA-degrading enzymes and non-specific activation of the innate immune response. In this essay, we investigate the evidence whether persistent infection with RNA viruses could be a major factor that affects the response to exogenous dsRNA in insects. The occurrence of RNA viruses in different insect groups will be discussed, as well as several mechanisms by which viruses could interfere with the process of RNAi. Finally, the impact of RNA virus infection on the design of dsRNA-based insect control strategies will be considered.
Keywords
persistent virus infection, RNAi, RNA virus, pest control, insects, innate immune response, DOUBLE-STRANDED-RNA, FLOCK HOUSE VIRUS, CYTOPLASMIC POLYHEDROSIS-VIRUS, DESERT LOCUST, SMALL SILENCING RNAS, BOMBYX-MORI, SCHISTOCERCA-GREGARIA, ANTIVIRAL IMMUNITY, CAENORHABDITIS-ELEGANS, DENGUE VIRUS

Downloads

  • fphys-04-00319.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 742.89 KB

Citation

Please use this url to cite or link to this publication:

MLA
Swevers, Luc, Jozef Vanden Broeck, and Guy Smagghe. “The Possible Impact of Persistent Virus Infection on the Function of the RNAi Machinery in Insects: a Hypothesis.” FRONTIERS IN PHYSIOLOGY 4 (2013): n. pag. Print.
APA
Swevers, Luc, Vanden Broeck, J., & Smagghe, G. (2013). The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis. FRONTIERS IN PHYSIOLOGY, 4.
Chicago author-date
Swevers, Luc, Jozef Vanden Broeck, and Guy Smagghe. 2013. “The Possible Impact of Persistent Virus Infection on the Function of the RNAi Machinery in Insects: a Hypothesis.” Frontiers in Physiology 4.
Chicago author-date (all authors)
Swevers, Luc, Jozef Vanden Broeck, and Guy Smagghe. 2013. “The Possible Impact of Persistent Virus Infection on the Function of the RNAi Machinery in Insects: a Hypothesis.” Frontiers in Physiology 4.
Vancouver
1.
Swevers L, Vanden Broeck J, Smagghe G. The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis. FRONTIERS IN PHYSIOLOGY. 2013;4.
IEEE
[1]
L. Swevers, J. Vanden Broeck, and G. Smagghe, “The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis,” FRONTIERS IN PHYSIOLOGY, vol. 4, 2013.
@article{5987194,
  abstract     = {RNAi experiments in insects are characterized by great variability in efficiency; for instance beetles and locusts are very amenable to dsRNA-mediated gene silencing, while other insect groups, most notably lepidopterans, are more refractory to RNAi. Several factors can be forwarded that could affect the efficiency of RNAi, such as the composition and function of the intracellular RNAi machinery, the mechanism of dsRNA uptake, the presence of dsRNA- and siRNA-degrading enzymes and non-specific activation of the innate immune response. In this essay, we investigate the evidence whether persistent infection with RNA viruses could be a major factor that affects the response to exogenous dsRNA in insects. The occurrence of RNA viruses in different insect groups will be discussed, as well as several mechanisms by which viruses could interfere with the process of RNAi. Finally, the impact of RNA virus infection on the design of dsRNA-based insect control strategies will be considered.},
  articleno    = {319},
  author       = {Swevers, Luc and Vanden Broeck, Jozef and Smagghe, Guy},
  issn         = {1664-042X},
  journal      = {FRONTIERS IN PHYSIOLOGY},
  keywords     = {persistent virus infection,RNAi,RNA virus,pest control,insects,innate immune response,DOUBLE-STRANDED-RNA,FLOCK HOUSE VIRUS,CYTOPLASMIC POLYHEDROSIS-VIRUS,DESERT LOCUST,SMALL SILENCING RNAS,BOMBYX-MORI,SCHISTOCERCA-GREGARIA,ANTIVIRAL IMMUNITY,CAENORHABDITIS-ELEGANS,DENGUE VIRUS},
  language     = {eng},
  pages        = {15},
  title        = {The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis},
  url          = {http://dx.doi.org/10.3389/fphys.2013.00319},
  volume       = {4},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: