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8-prenylnaringenin and tamoxifen inhibit the shedding of irradiated epithelial cells and increase the latency period of radiation-induced oral mucositis

Tine De Ryck, Annouchka Van Impe, Barbara Vanhoecke, Arne Heyerick UGent, Luc Vakaet UGent, Wilfried De Neve UGent, Doreen Müller, Margret Schmidt, Wolfgang Dörr and Marc Bracke UGent (2015) STRAHLENTHERAPIE UND ONKOLOGIE. 191(5). p.429-436
abstract
Purpose: The major component in the pathogenesis of oral radiation-induced mucositis is progressive epithelial hypoplasia and eventual ulceration. Irradiation inhibits cell proliferation, while cell loss at the surface continues. We conceived to slow down this desquamation by increasing intercellular adhesion, regulated by the E-cadherin/catenin complex. We investigated if 8-prenylnaringenin (8-PN) or tamoxifen (TAM) decrease the shedding of irradiated human buccal epithelial cells in vitro and thus delay the ulcerative phase of radiation-induced mucositis in vivo. Material and methods: In vitro, aggregates of buccal epithelial cells were irradiated and cultured in suspension for 11 days. 8-PN or TAM were investigated regarding their effect on cell shedding. In vivo, the lower tongue surface of mice was irradiated with graded single doses of 25 kV X-rays. The incidence, latency, and duration of the resulting mucosal ulcerations were analyzed after topical treatment with 8-PN, TAM or solvent. Results: 8-PN or TAM prevented the volume reduction of the irradiated cell aggregates during the incubation period. This was the result of a higher residual cell number in the treated versus the untreated irradiated aggregates. In vivo, topical treatment with 8-PN or TAM significantly increased the latency of mucositis from 10.9 to 12.1 and 12.4 days respectively, while the ulcer incidence was unchanged. Conclusion: 8-PN and TAM prevent volume reduction of irradiated cell aggregates in suspension culture. In the tongues of mice, these compounds increase the latency period. This suggests a role for these compounds for the amelioration of radiation-induced mucositis in the treatment of head and neck tumors.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Head and neck cancer, Radiotherapy, Mouse tongue model, Buccal epithelium, Cell aggregates, E-cadherin, KERATINOCYTE GROWTH-FACTOR, MAMMARY-CARCINOMA CELLS, NECK-CANCER, FACTOR PALIFERMIN, E-CADHERIN, BREAST-CANCER, HEAD, RADIOTHERAPY, MICE, MAINTENANCE
journal title
STRAHLENTHERAPIE UND ONKOLOGIE
Strahlenther. Onkol.
volume
191
issue
5
pages
429 - 436
Web of Science type
Article
Web of Science id
000353911900005
JCR category
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
JCR impact factor
2.898 (2015)
JCR rank
29/124 (2015)
JCR quartile
1 (2015)
ISSN
0179-7158
DOI
10.1007/s00066-014-0782-2
language
English
UGent publication?
yes
classification
A1
additional info
the last two authors are equally contributing senior authors
copyright statement
I have transferred the copyright for this publication to the publisher
id
5986191
handle
http://hdl.handle.net/1854/LU-5986191
date created
2015-06-08 15:19:22
date last changed
2016-12-19 15:47:01
@article{5986191,
  abstract     = {Purpose: The major component in the pathogenesis of oral radiation-induced mucositis is progressive epithelial hypoplasia and eventual ulceration. Irradiation inhibits cell proliferation, while cell loss at the surface continues. We conceived to slow down this desquamation by increasing intercellular adhesion, regulated by the E-cadherin/catenin complex. We investigated if 8-prenylnaringenin (8-PN) or tamoxifen (TAM) decrease the shedding of irradiated human buccal epithelial cells in vitro and thus delay the ulcerative phase of radiation-induced mucositis in vivo. 
Material and methods: In vitro, aggregates of buccal epithelial cells were irradiated and cultured in suspension for 11 days. 8-PN or TAM were investigated regarding their effect on cell shedding. In vivo, the lower tongue surface of mice was irradiated with graded single doses of 25 kV X-rays. The incidence, latency, and duration of the resulting mucosal ulcerations were analyzed after topical treatment with 8-PN, TAM or solvent. 
Results: 8-PN or TAM prevented the volume reduction of the irradiated cell aggregates during the incubation period. This was the result of a higher residual cell number in the treated versus the untreated irradiated aggregates. In vivo, topical treatment with 8-PN or TAM significantly increased the latency of mucositis from 10.9 to 12.1 and 12.4 days respectively, while the ulcer incidence was unchanged. 
Conclusion: 8-PN and TAM prevent volume reduction of irradiated cell aggregates in suspension culture. In the tongues of mice, these compounds increase the latency period. This suggests a role for these compounds for the amelioration of radiation-induced mucositis in the treatment of head and neck tumors.},
  author       = {De Ryck, Tine and Van Impe, Annouchka and Vanhoecke, Barbara and Heyerick, Arne and Vakaet, Luc and De Neve, Wilfried and M{\"u}ller, Doreen and Schmidt, Margret and D{\"o}rr, Wolfgang and Bracke, Marc},
  issn         = {0179-7158},
  journal      = {STRAHLENTHERAPIE UND ONKOLOGIE},
  keyword      = {Head and neck cancer,Radiotherapy,Mouse tongue model,Buccal epithelium,Cell aggregates,E-cadherin,KERATINOCYTE GROWTH-FACTOR,MAMMARY-CARCINOMA CELLS,NECK-CANCER,FACTOR PALIFERMIN,E-CADHERIN,BREAST-CANCER,HEAD,RADIOTHERAPY,MICE,MAINTENANCE},
  language     = {eng},
  number       = {5},
  pages        = {429--436},
  title        = {8-prenylnaringenin and tamoxifen inhibit the shedding of irradiated epithelial cells and increase the latency period of radiation-induced oral mucositis},
  url          = {http://dx.doi.org/10.1007/s00066-014-0782-2},
  volume       = {191},
  year         = {2015},
}

Chicago
De Ryck, Tine, Annouchka Van Impe, Barbara Vanhoecke, Arne Heyerick, Luc Vakaet, Wilfried De Neve, Doreen Müller, Margret Schmidt, Wolfgang Dörr, and Marc Bracke. 2015. “8-prenylnaringenin and Tamoxifen Inhibit the Shedding of Irradiated Epithelial Cells and Increase the Latency Period of Radiation-induced Oral Mucositis.” Strahlentherapie Und Onkologie 191 (5): 429–436.
APA
De Ryck, T., Van Impe, A., Vanhoecke, B., Heyerick, A., Vakaet, L., De Neve, W., Müller, D., et al. (2015). 8-prenylnaringenin and tamoxifen inhibit the shedding of irradiated epithelial cells and increase the latency period of radiation-induced oral mucositis. STRAHLENTHERAPIE UND ONKOLOGIE, 191(5), 429–436.
Vancouver
1.
De Ryck T, Van Impe A, Vanhoecke B, Heyerick A, Vakaet L, De Neve W, et al. 8-prenylnaringenin and tamoxifen inhibit the shedding of irradiated epithelial cells and increase the latency period of radiation-induced oral mucositis. STRAHLENTHERAPIE UND ONKOLOGIE. 2015;191(5):429–36.
MLA
De Ryck, Tine, Annouchka Van Impe, Barbara Vanhoecke, et al. “8-prenylnaringenin and Tamoxifen Inhibit the Shedding of Irradiated Epithelial Cells and Increase the Latency Period of Radiation-induced Oral Mucositis.” STRAHLENTHERAPIE UND ONKOLOGIE 191.5 (2015): 429–436. Print.