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Plant hormone signalling through the eye of the mass spectrometer

Alan Walton (UGent) , Elisabeth Stes (UGent) , Ive De Smet (UGent) , Sofie Goormachtig (UGent) and Kris Gevaert (UGent)
(2015) PROTEOMICS. 15(5-6). p.1113-1126
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Abstract
Plant growth and development are regulated by hormones and the associated signalling pathways share several common steps, the first being the detection of the signal by receptor proteins. This typically leads to conformational changes in the receptor, thereby modifying its spectrum of interaction partners. Next, secondary signals are transmitted via rapid post-translational cascades, such as targeted phosphorylation or ubiquitination, resulting in the activation/deactivation, relocalization or degradation of target proteins. These events finally give rise to the signal-dependent read-out, such as changes in gene expression and regulation of protein activity. So far, the majority of studies aimed at unravelling hormone signalling pathways in plants relied on genetic or transcriptomic approaches. During the last decade however, MS-driven proteomic methods became increasingly popular tools in plant research as they reveal the specific mechanisms controlled by phytohormones, which for a large part occur on the level of the proteome. Here, we provide an up-to-date review on the growing body of work in these areas using MS-based techniques, with a focus on nonpeptide plant hormones.
Keywords
POSTTRANSLATIONAL MODIFICATIONS, QUANTITATIVE PROTEOMICS, ABSCISIC-ACID, ARABIDOPSIS-THALIANA, BOX PROTEIN TIR1, DIFFERENCE GEL-ELECTROPHORESIS, TANDEM AFFINITY PURIFICATION, Signalling, PTMs, Plant proteomics, Plant hormone, Affinity purification, UBIQUITYLATION TARGETS, UBIQUITINATION SITES, REGULATED PROTEINS

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Chicago
Walton, Alan, Elisabeth Stes, Ive De Smet, Sofie Goormachtig, and Kris Gevaert. 2015. “Plant Hormone Signalling Through the Eye of the Mass Spectrometer.” Proteomics 15 (5-6): 1113–1126.
APA
Walton, A., Stes, E., De Smet, I., Goormachtig, S., & Gevaert, K. (2015). Plant hormone signalling through the eye of the mass spectrometer. PROTEOMICS, 15(5-6), 1113–1126.
Vancouver
1.
Walton A, Stes E, De Smet I, Goormachtig S, Gevaert K. Plant hormone signalling through the eye of the mass spectrometer. PROTEOMICS. 2015;15(5-6):1113–26.
MLA
Walton, Alan, Elisabeth Stes, Ive De Smet, et al. “Plant Hormone Signalling Through the Eye of the Mass Spectrometer.” PROTEOMICS 15.5-6 (2015): 1113–1126. Print.
@article{5986181,
  abstract     = {Plant growth and development are regulated by hormones and the associated signalling pathways share several common steps, the first being the detection of the signal by receptor proteins. This typically leads to conformational changes in the receptor, thereby modifying its spectrum of interaction partners. Next, secondary signals are transmitted via rapid post-translational cascades, such as targeted phosphorylation or ubiquitination, resulting in the activation/deactivation, relocalization or degradation of target proteins. These events finally give rise to the signal-dependent read-out, such as changes in gene expression and regulation of protein activity. So far, the majority of studies aimed at unravelling hormone signalling pathways in plants relied on genetic or transcriptomic approaches. During the last decade however, MS-driven proteomic methods became increasingly popular tools in plant research as they reveal the specific mechanisms controlled by phytohormones, which for a large part occur on the level of the proteome. Here, we provide an up-to-date review on the growing body of work in these areas using MS-based techniques, with a focus on nonpeptide plant hormones.},
  author       = {Walton, Alan and Stes, Elisabeth and De Smet, Ive and Goormachtig, Sofie and Gevaert, Kris},
  issn         = {1615-9853},
  journal      = {PROTEOMICS},
  keyword      = {POSTTRANSLATIONAL MODIFICATIONS,QUANTITATIVE PROTEOMICS,ABSCISIC-ACID,ARABIDOPSIS-THALIANA,BOX PROTEIN TIR1,DIFFERENCE GEL-ELECTROPHORESIS,TANDEM AFFINITY PURIFICATION,Signalling,PTMs,Plant proteomics,Plant hormone,Affinity purification,UBIQUITYLATION TARGETS,UBIQUITINATION SITES,REGULATED PROTEINS},
  language     = {eng},
  number       = {5-6},
  pages        = {1113--1126},
  title        = {Plant hormone signalling through the eye of the mass spectrometer},
  url          = {http://dx.doi.org/10.1002/pmic.201400403},
  volume       = {15},
  year         = {2015},
}

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