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Protein methionine sulfoxide dynamics in Arabidopsis thaliana under oxidative stress

(2015) MOLECULAR & CELLULAR PROTEOMICS. 14(5). p.1217-1229
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Reactive oxygen species such as hydrogen peroxide can modify proteins via direct oxidation of their sulfur-containing amino acids, cysteine and methionine. Methionine oxidation, studied here, is a reversible posttranslational modification that is emerging as a mechanism by which proteins perceive oxidative stress and function in redox signaling. Identification of proteins with oxidized methionines is the first prerequisite toward understanding the functional effect of methionine oxidation on proteins and the biological processes in which they are involved. Here, we describe a proteome-wide study of in vivo protein-bound methionine oxidation in plants upon oxidative stress using Arabidopsis thaliana catalase 2 knock-out plants as a model system. We identified over 500 sites of oxidation in about 400 proteins and quantified the differences in oxidation between wild-type and catalase 2 knock-out plants. We show that the activity of two plant-specific glutathione S-transferases, GSTF9 and GSTT23, is significantly reduced upon oxidation. And, by sampling over time, we mapped the dynamics of methionine oxidation and gained new insights into this complex and dynamic landscape of a part of the plant proteome that is sculpted by oxidative stress.
Keywords
TRANSFERASES, RESPONSES, TOLERANCE, SELECTIVITY, PLANTS, REDUCTASE-A, REDOX REGULATION, GENE-EXPRESSION, TYROSINE NITRATION, HYDROGEN-PEROXIDE

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Citation

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Chicago
Jacques, Silke, Bart Ghesquière, Pieter-Jan De Bock, Hans Demol, Khadija Wahni, Patrick Willems, Joris Messens, Frank Van Breusegem, and Kris Gevaert. 2015. “Protein Methionine Sulfoxide Dynamics in Arabidopsis Thaliana Under Oxidative Stress.” Molecular & Cellular Proteomics 14 (5): 1217–1229.
APA
Jacques, Silke, Ghesquière, B., De Bock, P.-J., Demol, H., Wahni, K., Willems, P., Messens, J., et al. (2015). Protein methionine sulfoxide dynamics in Arabidopsis thaliana under oxidative stress. MOLECULAR & CELLULAR PROTEOMICS, 14(5), 1217–1229.
Vancouver
1.
Jacques S, Ghesquière B, De Bock P-J, Demol H, Wahni K, Willems P, et al. Protein methionine sulfoxide dynamics in Arabidopsis thaliana under oxidative stress. MOLECULAR & CELLULAR PROTEOMICS. 2015;14(5):1217–29.
MLA
Jacques, Silke, Bart Ghesquière, Pieter-Jan De Bock, et al. “Protein Methionine Sulfoxide Dynamics in Arabidopsis Thaliana Under Oxidative Stress.” MOLECULAR & CELLULAR PROTEOMICS 14.5 (2015): 1217–1229. Print.
@article{5986018,
  abstract     = {Reactive oxygen species such as hydrogen peroxide can modify proteins via direct oxidation of their sulfur-containing amino acids, cysteine and methionine. Methionine oxidation, studied here, is a reversible posttranslational modification that is emerging as a mechanism by which proteins perceive oxidative stress and function in redox signaling. Identification of proteins with oxidized methionines is the first prerequisite toward understanding the functional effect of methionine oxidation on proteins and the biological processes in which they are involved. Here, we describe a proteome-wide study of in vivo protein-bound methionine oxidation in plants upon oxidative stress using Arabidopsis thaliana catalase 2 knock-out plants as a model system. We identified over 500 sites of oxidation in about 400 proteins and quantified the differences in oxidation between wild-type and catalase 2 knock-out plants. We show that the activity of two plant-specific glutathione S-transferases, GSTF9 and GSTT23, is significantly reduced upon oxidation. And, by sampling over time, we mapped the dynamics of methionine oxidation and gained new insights into this complex and dynamic landscape of a part of the plant proteome that is sculpted by oxidative stress.},
  author       = {Jacques, Silke and Ghesqui{\`e}re, Bart and De Bock, Pieter-Jan and Demol, Hans and Wahni, Khadija and Willems, Patrick and Messens, Joris and Van Breusegem, Frank and Gevaert, Kris},
  issn         = {1535-9476},
  journal      = {MOLECULAR \& CELLULAR PROTEOMICS},
  language     = {eng},
  number       = {5},
  pages        = {1217--1229},
  title        = {Protein methionine sulfoxide dynamics in Arabidopsis thaliana under oxidative stress},
  url          = {http://dx.doi.org/10.1074/mcp.M114.043729},
  volume       = {14},
  year         = {2015},
}

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