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Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae)

(2015) ECOTOXICOLOGY. 24(1). p.130-142
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Abstract
Azadirachtin is a biorational insecticide commonly reported as selective to a range of beneficial insects. Nonetheless, only few studies have been carried out with pollinators, usually emphasizing the honeybee Apis mellifera and neglecting other important pollinator species such as the bumblebee Bombus terrestris. Here, lethal and sublethal effects of azadirachtin were studied on B. terrestris via oral exposure in the laboratory to bring out the potential risks of the compound to this important pollinator. The compound was tested at different concentrations above and below the maximum concentration that is used in the field (32 mg L-1). As most important results, azadirachtin repelled bumblebee workers in a concentration-dependent manner. The median repellence concentration (RC50) was estimated as 504 mg L-1. Microcolonies chronically exposed to azadirachtin via treated sugar water during 11 weeks in the laboratory exhibited a high mortality ranging from 32 to 100 % with a range of concentrations between 3.2 and 320 mg L-1. Moreover, no reproduction was scored when concentrations were higher than 3.2 mg L-1. At 3.2 mg L-1, azadirachtin significantly inhibited the egg-laying and, consequently, the production of drones during 6 weeks. Ovarian length decreased with the increase of the azadirachtin concentration. When azadirachtin was tested under an experimental setup in the laboratory where bumblebees need to forage for food, the sublethal effects were stronger as the numbers of drones were reduced already with a concentration of 0.64 mg L-1. Besides, a negative correlation was found between the body mass of male offspring and azadirachtin concentration. In conclusion, our results as performed in the laboratory demonstrated that azadirachtin can affect B. terrestris with a range of sublethal effects. Taking into account that sublethal effects are as important as lethal effects for the development and survival of the colonies of B. terrestris, this study confirms the need to test compounds on their safety, especially when they have to perform complex tasks such as foraging. The latter agrees with the recent European Food Safety Authority guidelines to assess 'potentially deleterious' compounds for sublethal effects on behavior.
Keywords
Chronic oral exposure, Bumblebee, Insect growth regulator, Neem, Repellence effect, Reproduction, APIS-MELLIFERA L., LABIDURA-RIPARIA, HUMID TROPICS, ADULT FEMALES, NEEM EXTRACT, HONEY-BEES, INSECTICIDES, PESTICIDES, GROWTH, CONSERVATION

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Chicago
Faria Barbosa, Wagner, Laurens De Meyer, Raul Narciso C Guedes, and Guy Smagghe. 2015. “Lethal and Sublethal Effects of Azadirachtin on the Bumblebee Bombus Terrestris (Hymenoptera: Apidae).” Ecotoxicology 24 (1): 130–142.
APA
Faria Barbosa, W., De Meyer, L., Guedes, R. N. C., & Smagghe, G. (2015). Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae). ECOTOXICOLOGY, 24(1), 130–142.
Vancouver
1.
Faria Barbosa W, De Meyer L, Guedes RNC, Smagghe G. Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae). ECOTOXICOLOGY. 2015;24(1):130–42.
MLA
Faria Barbosa, Wagner, Laurens De Meyer, Raul Narciso C Guedes, et al. “Lethal and Sublethal Effects of Azadirachtin on the Bumblebee Bombus Terrestris (Hymenoptera: Apidae).” ECOTOXICOLOGY 24.1 (2015): 130–142. Print.
@article{5985961,
  abstract     = {Azadirachtin is a biorational insecticide commonly reported as selective to a range of beneficial insects. Nonetheless, only few studies have been carried out with pollinators, usually emphasizing the honeybee Apis mellifera and neglecting other important pollinator species such as the bumblebee Bombus terrestris. Here, lethal and sublethal effects of azadirachtin were studied on B. terrestris via oral exposure in the laboratory to bring out the potential risks of the compound to this important pollinator. The compound was tested at different concentrations above and below the maximum concentration that is used in the field (32 mg L-1). As most important results, azadirachtin repelled bumblebee workers in a concentration-dependent manner. The median repellence concentration (RC50) was estimated as 504 mg L-1. Microcolonies chronically exposed to azadirachtin via treated sugar water during 11 weeks in the laboratory exhibited a high mortality ranging from 32 to 100 \% with a range of concentrations between 3.2 and 320 mg L-1. Moreover, no reproduction was scored when concentrations were higher than 3.2 mg L-1. At 3.2 mg L-1, azadirachtin significantly inhibited the egg-laying and, consequently, the production of drones during 6 weeks. Ovarian length decreased with the increase of the azadirachtin concentration. When azadirachtin was tested under an experimental setup in the laboratory where bumblebees need to forage for food, the sublethal effects were stronger as the numbers of drones were reduced already with a concentration of 0.64 mg L-1. Besides, a negative correlation was found between the body mass of male offspring and azadirachtin concentration. In conclusion, our results as performed in the laboratory demonstrated that azadirachtin can affect B. terrestris with a range of sublethal effects. Taking into account that sublethal effects are as important as lethal effects for the development and survival of the colonies of B. terrestris, this study confirms the need to test compounds on their safety, especially when they have to perform complex tasks such as foraging. The latter agrees with the recent European Food Safety Authority guidelines to assess 'potentially deleterious' compounds for sublethal effects on behavior.},
  author       = {Faria Barbosa, Wagner and De Meyer, Laurens and Guedes, Raul Narciso C and Smagghe, Guy},
  issn         = {0963-9292},
  journal      = {ECOTOXICOLOGY},
  keyword      = {Chronic oral exposure,Bumblebee,Insect growth regulator,Neem,Repellence effect,Reproduction,APIS-MELLIFERA L.,LABIDURA-RIPARIA,HUMID TROPICS,ADULT FEMALES,NEEM EXTRACT,HONEY-BEES,INSECTICIDES,PESTICIDES,GROWTH,CONSERVATION},
  language     = {eng},
  number       = {1},
  pages        = {130--142},
  title        = {Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae)},
  url          = {http://dx.doi.org/10.1007/s10646-014-1365-9},
  volume       = {24},
  year         = {2015},
}

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