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Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae)

Wagner Faria Barbosa, Laurens De Meyer, Raul Narciso C Guedes and Guy Smagghe UGent (2015) ECOTOXICOLOGY. 24(1). p.130-142
abstract
Azadirachtin is a biorational insecticide commonly reported as selective to a range of beneficial insects. Nonetheless, only few studies have been carried out with pollinators, usually emphasizing the honeybee Apis mellifera and neglecting other important pollinator species such as the bumblebee Bombus terrestris. Here, lethal and sublethal effects of azadirachtin were studied on B. terrestris via oral exposure in the laboratory to bring out the potential risks of the compound to this important pollinator. The compound was tested at different concentrations above and below the maximum concentration that is used in the field (32 mg L-1). As most important results, azadirachtin repelled bumblebee workers in a concentration-dependent manner. The median repellence concentration (RC50) was estimated as 504 mg L-1. Microcolonies chronically exposed to azadirachtin via treated sugar water during 11 weeks in the laboratory exhibited a high mortality ranging from 32 to 100 % with a range of concentrations between 3.2 and 320 mg L-1. Moreover, no reproduction was scored when concentrations were higher than 3.2 mg L-1. At 3.2 mg L-1, azadirachtin significantly inhibited the egg-laying and, consequently, the production of drones during 6 weeks. Ovarian length decreased with the increase of the azadirachtin concentration. When azadirachtin was tested under an experimental setup in the laboratory where bumblebees need to forage for food, the sublethal effects were stronger as the numbers of drones were reduced already with a concentration of 0.64 mg L-1. Besides, a negative correlation was found between the body mass of male offspring and azadirachtin concentration. In conclusion, our results as performed in the laboratory demonstrated that azadirachtin can affect B. terrestris with a range of sublethal effects. Taking into account that sublethal effects are as important as lethal effects for the development and survival of the colonies of B. terrestris, this study confirms the need to test compounds on their safety, especially when they have to perform complex tasks such as foraging. The latter agrees with the recent European Food Safety Authority guidelines to assess 'potentially deleterious' compounds for sublethal effects on behavior.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chronic oral exposure, Bumblebee, Insect growth regulator, Neem, Repellence effect, Reproduction, APIS-MELLIFERA L., LABIDURA-RIPARIA, HUMID TROPICS, ADULT FEMALES, NEEM EXTRACT, HONEY-BEES, INSECTICIDES, PESTICIDES, GROWTH, CONSERVATION
journal title
ECOTOXICOLOGY
Ecotoxicology
volume
24
issue
1
pages
130 - 142
Web of Science type
Article
Web of Science id
000347693100012
JCR category
ENVIRONMENTAL SCIENCES
JCR impact factor
2.329 (2015)
JCR rank
81/225 (2015)
JCR quartile
2 (2015)
ISSN
0963-9292
DOI
10.1007/s10646-014-1365-9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5985961
handle
http://hdl.handle.net/1854/LU-5985961
date created
2015-06-08 15:03:36
date last changed
2016-12-19 15:43:17
@article{5985961,
  abstract     = {Azadirachtin is a biorational insecticide commonly reported as selective to a range of beneficial insects. Nonetheless, only few studies have been carried out with pollinators, usually emphasizing the honeybee Apis mellifera and neglecting other important pollinator species such as the bumblebee Bombus terrestris. Here, lethal and sublethal effects of azadirachtin were studied on B. terrestris via oral exposure in the laboratory to bring out the potential risks of the compound to this important pollinator. The compound was tested at different concentrations above and below the maximum concentration that is used in the field (32 mg L-1). As most important results, azadirachtin repelled bumblebee workers in a concentration-dependent manner. The median repellence concentration (RC50) was estimated as 504 mg L-1. Microcolonies chronically exposed to azadirachtin via treated sugar water during 11 weeks in the laboratory exhibited a high mortality ranging from 32 to 100 \% with a range of concentrations between 3.2 and 320 mg L-1. Moreover, no reproduction was scored when concentrations were higher than 3.2 mg L-1. At 3.2 mg L-1, azadirachtin significantly inhibited the egg-laying and, consequently, the production of drones during 6 weeks. Ovarian length decreased with the increase of the azadirachtin concentration. When azadirachtin was tested under an experimental setup in the laboratory where bumblebees need to forage for food, the sublethal effects were stronger as the numbers of drones were reduced already with a concentration of 0.64 mg L-1. Besides, a negative correlation was found between the body mass of male offspring and azadirachtin concentration. In conclusion, our results as performed in the laboratory demonstrated that azadirachtin can affect B. terrestris with a range of sublethal effects. Taking into account that sublethal effects are as important as lethal effects for the development and survival of the colonies of B. terrestris, this study confirms the need to test compounds on their safety, especially when they have to perform complex tasks such as foraging. The latter agrees with the recent European Food Safety Authority guidelines to assess 'potentially deleterious' compounds for sublethal effects on behavior.},
  author       = {Faria Barbosa, Wagner and De Meyer, Laurens and Guedes, Raul Narciso C and Smagghe, Guy},
  issn         = {0963-9292},
  journal      = {ECOTOXICOLOGY},
  keyword      = {Chronic oral exposure,Bumblebee,Insect growth regulator,Neem,Repellence effect,Reproduction,APIS-MELLIFERA L.,LABIDURA-RIPARIA,HUMID TROPICS,ADULT FEMALES,NEEM EXTRACT,HONEY-BEES,INSECTICIDES,PESTICIDES,GROWTH,CONSERVATION},
  language     = {eng},
  number       = {1},
  pages        = {130--142},
  title        = {Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae)},
  url          = {http://dx.doi.org/10.1007/s10646-014-1365-9},
  volume       = {24},
  year         = {2015},
}

Chicago
Faria Barbosa, Wagner, Laurens De Meyer, Raul Narciso C Guedes, and Guy Smagghe. 2015. “Lethal and Sublethal Effects of Azadirachtin on the Bumblebee Bombus Terrestris (Hymenoptera: Apidae).” Ecotoxicology 24 (1): 130–142.
APA
Faria Barbosa, W., De Meyer, L., Guedes, R. N. C., & Smagghe, G. (2015). Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae). ECOTOXICOLOGY, 24(1), 130–142.
Vancouver
1.
Faria Barbosa W, De Meyer L, Guedes RNC, Smagghe G. Lethal and sublethal effects of azadirachtin on the bumblebee Bombus terrestris (Hymenoptera: Apidae). ECOTOXICOLOGY. 2015;24(1):130–42.
MLA
Faria Barbosa, Wagner, Laurens De Meyer, Raul Narciso C Guedes, et al. “Lethal and Sublethal Effects of Azadirachtin on the Bumblebee Bombus Terrestris (Hymenoptera: Apidae).” ECOTOXICOLOGY 24.1 (2015): 130–142. Print.