Advanced search
1 file | 7.69 MB Add to list

The immunity raised by recent European subtype 1 PRRSV strains allows a better replication of East European subtype 3 PRRSV strain Lena than the immunity raised by an older strain: increased risk for spatial expansion of PRRSV Lena-like strains

Author
Organization
Project
PORRSCON (New tools and approaches to control Porcine Reproductive and Respiratory Syndrome (PRRS) in the EU and Asia)
Abstract
The spatial distribution of PRRSV-1 subtypes in Europe is quite stable, most probably due to a strong population immunity induced by the local PRRSV strains. In this study, we evaluated the potential of the immunity induced by several West European subtype 1 PRRSV strains (2007 isolate 07V063 and 2013 isolates 13V091 and 13V117) to provide a protection against the highly virulent East European subtype 3 PRRSV strain Lena. Eleven-week-old pigs were inoculated with subtype 1 PRRSV strains (07V063, 13V091 or 13V117). Seven weeks later, the pigs were challenged with PRRSV strain Lena. Clinical, virological and serological parameters were monitored upon challenge. Number of fever days was higher (P < 0.05) in the non-immune control group (7.6 ± 1.7 days) compared to animals from immune groups (07V063-immune: 4.0 ± 1.2 days, 13V091-immune: 4.6 ± 1.1 days, 13V117-immune: 4.0 ± 2.9 days). In all groups, protection was characterized by reduction (P < 0.05) of AUC values of nasal shedding (control: 14.6 ± 5.6, 07V063-immune: 3.4 ± 3.4, 13V091-immune: 8.9 ± 6.1, 13V117-immune: 8.0 ± 6.1) and viremia (control: 28.1 ± 11.0, 07V063-immune: 5.4 ± 4.4, 13V091-immune: 9.0 ± 1.5, 13V117-immune: 8.3 ± 4.8). Reduction of respiratory disease, nasal shedding (mean AUC and mean peak values) and viremia (mean AUC and mean peak values) was more pronounced in 07V063-immune (P < 0.05) than in 13V091-immune and 13V117-immune animals. Inoculation of animals with subtype 1 PRRSV strains caused a priming of Lena-specific VN antibody response. Upon challenge with PRRSV Lena a serological booster effect was observed for neutralizing antibodies against strains used for the first inoculation. Our results indicate that immunity elicited by inoculation with subtype 1 PRRSV strains can partially protect against antigenically divergent subtype 3 strains. We conclude that the lower protection level elicited by recently isolated subtype 1 PRRSV strains may facilitate spatial expansion of subtype 3 strains from East Europe to West Europe.
Keywords
PRRS, Adaptive immunity, PRRSV, Subtype, Spatial distribution, Lena, Virus shedding, Viremia, Cross-protection

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 7.69 MB

Citation

Please use this url to cite or link to this publication:

MLA
Trus, Ivan, Ilias Frydas, Vishwanatha Reddy Avalakuppa Papi Reddy, et al. “The Immunity Raised by Recent European Subtype 1 PRRSV Strains Allows a Better Replication of East European Subtype 3 PRRSV Strain Lena Than the Immunity Raised by an Older Strain: Increased Risk for Spatial Expansion of PRRSV Lena-like Strains.” International PRRS Congress : Abstract Book. 2015. 37–37. Print.
APA
Trus, I., Frydas, I., Avalakuppa Papi Reddy, V. R., Bonckaert, C., Li, Y., Kvisgaard, L. K., Larsen, L. E., et al. (2015). The immunity raised by recent European subtype 1 PRRSV strains allows a better replication of East European subtype 3 PRRSV strain Lena than the immunity raised by an older strain: increased risk for spatial expansion of PRRSV Lena-like strains. International PRRS congress : abstract book (pp. 37–37). Presented at the 2015 International Porcine Reproductive and Respiratory Syndrome congress (PRRS 2015).
Chicago author-date
Trus, Ivan, Ilias Frydas, Vishwanatha Reddy Avalakuppa Papi Reddy, Caroline Bonckaert, Yewei Li, Lise K Kvisgaard, Lars E Larsen, and Hans Nauwynck. 2015. “The Immunity Raised by Recent European Subtype 1 PRRSV Strains Allows a Better Replication of East European Subtype 3 PRRSV Strain Lena Than the Immunity Raised by an Older Strain: Increased Risk for Spatial Expansion of PRRSV Lena-like Strains.” In International PRRS Congress : Abstract Book, 37–37.
Chicago author-date (all authors)
Trus, Ivan, Ilias Frydas, Vishwanatha Reddy Avalakuppa Papi Reddy, Caroline Bonckaert, Yewei Li, Lise K Kvisgaard, Lars E Larsen, and Hans Nauwynck. 2015. “The Immunity Raised by Recent European Subtype 1 PRRSV Strains Allows a Better Replication of East European Subtype 3 PRRSV Strain Lena Than the Immunity Raised by an Older Strain: Increased Risk for Spatial Expansion of PRRSV Lena-like Strains.” In International PRRS Congress : Abstract Book, 37–37.
Vancouver
1.
Trus I, Frydas I, Avalakuppa Papi Reddy VR, Bonckaert C, Li Y, Kvisgaard LK, et al. The immunity raised by recent European subtype 1 PRRSV strains allows a better replication of East European subtype 3 PRRSV strain Lena than the immunity raised by an older strain: increased risk for spatial expansion of PRRSV Lena-like strains. International PRRS congress : abstract book. 2015. p. 37–37.
IEEE
[1]
I. Trus et al., “The immunity raised by recent European subtype 1 PRRSV strains allows a better replication of East European subtype 3 PRRSV strain Lena than the immunity raised by an older strain: increased risk for spatial expansion of PRRSV Lena-like strains,” in International PRRS congress : abstract book, Ghent, Belgium, 2015, pp. 37–37.
@inproceedings{5980407,
  abstract     = {The spatial distribution of PRRSV-1 subtypes in Europe is quite stable, most probably due to a strong population immunity induced by the local PRRSV strains. In this study, we evaluated the potential of the immunity induced by several West European subtype 1 PRRSV strains (2007 isolate 07V063 and 2013 isolates 13V091 and 13V117) to provide a protection against the highly virulent East European subtype 3 PRRSV strain Lena. Eleven-week-old pigs were inoculated with subtype 1 PRRSV strains (07V063, 13V091 or 13V117). Seven weeks later, the pigs were challenged with PRRSV strain Lena. Clinical, virological and serological parameters were monitored upon challenge. Number of fever days was higher (P < 0.05) in the non-immune control group (7.6 ± 1.7 days) compared to animals from immune groups (07V063-immune: 4.0 ± 1.2 days, 13V091-immune: 4.6 ± 1.1 days, 13V117-immune: 4.0 ± 2.9 days). In all groups, protection was characterized by reduction (P < 0.05) of AUC values of nasal shedding (control: 14.6 ± 5.6, 07V063-immune: 3.4 ± 3.4, 13V091-immune: 8.9 ± 6.1, 13V117-immune: 8.0 ± 6.1) and viremia (control: 28.1 ± 11.0, 07V063-immune: 5.4 ± 4.4, 13V091-immune: 9.0 ± 1.5, 13V117-immune: 8.3 ± 4.8). Reduction of respiratory disease, nasal shedding (mean AUC and mean peak values) and viremia (mean AUC and mean peak values) was more pronounced in 07V063-immune (P < 0.05) than in 13V091-immune and 13V117-immune animals. Inoculation of animals with subtype 1 PRRSV strains caused a priming of Lena-specific VN antibody response. Upon challenge with PRRSV Lena a serological booster effect was observed for neutralizing antibodies against strains used for the first inoculation. Our results indicate that immunity elicited by inoculation with subtype 1 PRRSV strains can partially protect against antigenically divergent subtype 3 strains. We conclude that the lower protection level elicited by recently isolated subtype 1 PRRSV strains may facilitate spatial expansion of subtype 3 strains from East Europe to West Europe.},
  articleno    = {abstract O.15},
  author       = {Trus, Ivan and Frydas, Ilias and Avalakuppa Papi Reddy, Vishwanatha Reddy and Bonckaert, Caroline and Li, Yewei and Kvisgaard, Lise K and Larsen, Lars E and Nauwynck, Hans},
  booktitle    = {International PRRS congress : abstract book},
  keywords     = {PRRS,Adaptive immunity,PRRSV,Subtype,Spatial distribution,Lena,Virus shedding,Viremia,Cross-protection},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {abstract O.15:37--abstract O.15:37},
  title        = {The immunity raised by recent European subtype 1 PRRSV strains allows a better replication of East European subtype 3 PRRSV strain Lena than the immunity raised by an older strain: increased risk for spatial expansion of PRRSV Lena-like strains},
  year         = {2015},
}