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Geobacter, Anaeromyxobacter and Anaerolineae populations are enriched on anodes of root exudate-driven microbial fuel cells in rice field soil

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Abstract
Plant-based sediment microbial fuel cells (PMFCs) couple the oxidation of root exudates in living rice plants to current production. We analysed the composition of the microbial community on anodes from PMFC with natural rice field soil as substratum for rice by analysing 16S rRNA as an indicator of microbial activity and diversity. Terminal restriction fragment length polymorphism (TRFLP) analysis indicated that the active bacterial community on anodes from PMFCs differed strongly compared with controls. Moreover, clones related to Deltaproteobacteria and Chloroflexi were highly abundant (49% and 21%, respectively) on PMFCs anodes. Geobacter (19%), Anaeromyxobacter (15%) and Anaerolineae (17%) populations were predominant on anodes with natural rice field soil and differed strongly from those previously detected with potting soil. In open circuit (OC) control PMFCs, not allowing electron transfer, Deltaproteobacteria (33%), Betaproteobacteria (20%), Chloroflexi (12%), Alphaproteobacteria (10%) and Firmicutes (10%) were detected. The presence of an electron accepting anode also had a strong influence on methanogenic archaea. Hydrogenotrophic methanogens were more active on PMFC (21%) than on OC controls (10%), whereas acetoclastic Methanosaetaceae were more active on OC controls (31%) compared with PMFCs (9%). In conclusion, electron accepting anodes and rice root exudates selected for distinct potential anode-reducing microbial populations in rice soil inoculated PMFC.
Keywords
ELECTRICITY PRODUCTION, 16S RIBOSOMAL-RNA, ELECTRON-TRANSFER, REDUCING MICROORGANISMS, HARVESTING ELECTRICITY, BACTERIAL COMMUNITIES, BIOFUEL CELLS, PERFORMANCE, COLONIZATION, DIVERSITY

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MLA
Cabezas, Angela et al. “Geobacter, Anaeromyxobacter and Anaerolineae Populations Are Enriched on Anodes of Root Exudate-driven Microbial Fuel Cells in Rice Field Soil.” ENVIRONMENTAL MICROBIOLOGY REPORTS 7.3 (2015): 489–497. Print.
APA
Cabezas, A., Pommerenke, B., Boon, N., & Friedrich, M. W. (2015). Geobacter, Anaeromyxobacter and Anaerolineae populations are enriched on anodes of root exudate-driven microbial fuel cells in rice field soil. ENVIRONMENTAL MICROBIOLOGY REPORTS, 7(3), 489–497.
Chicago author-date
Cabezas, Angela, Bianca Pommerenke, Nico Boon, and Michael W Friedrich. 2015. “Geobacter, Anaeromyxobacter and Anaerolineae Populations Are Enriched on Anodes of Root Exudate-driven Microbial Fuel Cells in Rice Field Soil.” Environmental Microbiology Reports 7 (3): 489–497.
Chicago author-date (all authors)
Cabezas, Angela, Bianca Pommerenke, Nico Boon, and Michael W Friedrich. 2015. “Geobacter, Anaeromyxobacter and Anaerolineae Populations Are Enriched on Anodes of Root Exudate-driven Microbial Fuel Cells in Rice Field Soil.” Environmental Microbiology Reports 7 (3): 489–497.
Vancouver
1.
Cabezas A, Pommerenke B, Boon N, Friedrich MW. Geobacter, Anaeromyxobacter and Anaerolineae populations are enriched on anodes of root exudate-driven microbial fuel cells in rice field soil. ENVIRONMENTAL MICROBIOLOGY REPORTS. 2015;7(3):489–97.
IEEE
[1]
A. Cabezas, B. Pommerenke, N. Boon, and M. W. Friedrich, “Geobacter, Anaeromyxobacter and Anaerolineae populations are enriched on anodes of root exudate-driven microbial fuel cells in rice field soil,” ENVIRONMENTAL MICROBIOLOGY REPORTS, vol. 7, no. 3, pp. 489–497, 2015.
@article{5970590,
  abstract     = {Plant-based sediment microbial fuel cells (PMFCs) couple the oxidation of root exudates in living rice plants to current production. We analysed the composition of the microbial community on anodes from PMFC with natural rice field soil as substratum for rice by analysing 16S rRNA as an indicator of microbial activity and diversity. Terminal restriction fragment length polymorphism (TRFLP) analysis indicated that the active bacterial community on anodes from PMFCs differed strongly compared with controls. Moreover, clones related to Deltaproteobacteria and Chloroflexi were highly abundant (49% and 21%, respectively) on PMFCs anodes. Geobacter (19%), Anaeromyxobacter (15%) and Anaerolineae (17%) populations were predominant on anodes with natural rice field soil and differed strongly from those previously detected with potting soil. In open circuit (OC) control PMFCs, not allowing electron transfer, Deltaproteobacteria (33%), Betaproteobacteria (20%), Chloroflexi (12%), Alphaproteobacteria (10%) and Firmicutes (10%) were detected. The presence of an electron accepting anode also had a strong influence on methanogenic archaea. Hydrogenotrophic methanogens were more active on PMFC (21%) than on OC controls (10%), whereas acetoclastic Methanosaetaceae were more active on OC controls (31%) compared with PMFCs (9%). In conclusion, electron accepting anodes and rice root exudates selected for distinct potential anode-reducing microbial populations in rice soil inoculated PMFC.},
  author       = {Cabezas, Angela and Pommerenke, Bianca and Boon, Nico and Friedrich, Michael W},
  issn         = {1758-2229},
  journal      = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
  keywords     = {ELECTRICITY PRODUCTION,16S RIBOSOMAL-RNA,ELECTRON-TRANSFER,REDUCING MICROORGANISMS,HARVESTING ELECTRICITY,BACTERIAL COMMUNITIES,BIOFUEL CELLS,PERFORMANCE,COLONIZATION,DIVERSITY},
  language     = {eng},
  number       = {3},
  pages        = {489--497},
  title        = {Geobacter, Anaeromyxobacter and Anaerolineae populations are enriched on anodes of root exudate-driven microbial fuel cells in rice field soil},
  url          = {http://dx.doi.org/10.1111/1758-2229.12277},
  volume       = {7},
  year         = {2015},
}

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