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Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates

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Organization
Project
PORRSCON (New tools and approaches to control Porcine Reproductive and Respiratory Syndrome (PRRS) in the EU and Asia)
Abstract
In this study, the pathogenic behavior of PRRSV 13V091 and 13V117, isolated in 2013 from two different Belgian farms with enzootic respiratory problems shortly after weaning in the nursery, were compared with the Belgian strain 07V063 isolated in 2007. Full-length genome sequencing was performed to identify their origin. Twelve weeks-old pigs were inoculated intranasally (IN) with 13V091, 13V117 or 07V063 (9 pigs/group). At 10 days post inoculation (dpi), 4 animals from each group were euthanized and tissues were collected for pathology, virological and serological analysis. 13V091 infection resulted in the highest respiratory disease scores and longest period of fever. Gross lung lesions were more pronounced for 13V091 (13%), than for 13V117 (7%) and 07V063 (11%). The nasal shedding and viremia was also most extensive with 13V091. The 13V091 group showed the highest virus replication in conchae, tonsils and retropharyngeal lymph nodes. 13V117 infection resulted in the lowest virus replication in lymphoid tissues. 13V091 showed higher numbers of sialoadhesin(-) infected cells/mm(2) in conchae, tonsils and spleen than 13V117 and 07V063. Neutralizing antibody response with 07V063 was stronger than with 13V091 and 13V117. It can be concluded that (i) 13V091 is a highly pathogenic type 1 subtype 1 PRRSV strain that replicates better than 07V063 and 13V117 and has a strong tropism for sialoadhesin(-) cells and (ii) despite the close genetic relationship between 13V117 and 07V063, 13V117 has an increased nasal replication and shedding, but a decreased replication in lymphoid tissues compared to 07V063.
Keywords
subtype 1, pathogenicity, type 1, 07V063, 13V117, PRRSV, 13V091, MYSTERY SWINE DISEASE, LELYSTAD VIRUS, COMPARATIVE PATHOGENICITY, MYCOPLASMA-HYOPNEUMONIAE, EPIDEMIC ABORTION, INFECTION, PIGS, REPLICATION, CELL, CHALLENGE

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MLA
Frydas, Ilias et al. “Different Clinical, Virological, Serological and Tissue Tropism Outcomes of Two New and One Old Belgian Type 1 Subtype 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Isolates.” VETERINARY RESEARCH 46 (2015): n. pag. Print.
APA
Frydas, I., Trus, I., Kvisgaard, L., Bonckaert, C., Avalakuppa Papi Reddy, V. R., Li, Y., Larsen, L., et al. (2015). Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates. VETERINARY RESEARCH, 46.
Chicago author-date
Frydas, Ilias, Ivan Trus, Lise Kvisgaard, Caroline Bonckaert, Vishwanatha Reddy Avalakuppa Papi Reddy, Yewei Li, Lars Larsen, and Hans Nauwynck. 2015. “Different Clinical, Virological, Serological and Tissue Tropism Outcomes of Two New and One Old Belgian Type 1 Subtype 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Isolates.” Veterinary Research 46.
Chicago author-date (all authors)
Frydas, Ilias, Ivan Trus, Lise Kvisgaard, Caroline Bonckaert, Vishwanatha Reddy Avalakuppa Papi Reddy, Yewei Li, Lars Larsen, and Hans Nauwynck. 2015. “Different Clinical, Virological, Serological and Tissue Tropism Outcomes of Two New and One Old Belgian Type 1 Subtype 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Isolates.” Veterinary Research 46.
Vancouver
1.
Frydas I, Trus I, Kvisgaard L, Bonckaert C, Avalakuppa Papi Reddy VR, Li Y, et al. Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates. VETERINARY RESEARCH. 2015;46.
IEEE
[1]
I. Frydas et al., “Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates,” VETERINARY RESEARCH, vol. 46, 2015.
@article{5965728,
  abstract     = {In this study, the pathogenic behavior of PRRSV 13V091 and 13V117, isolated in 2013 from two different Belgian farms with enzootic respiratory problems shortly after weaning in the nursery, were compared with the Belgian strain 07V063 isolated in 2007. Full-length genome sequencing was performed to identify their origin. Twelve weeks-old pigs were inoculated intranasally (IN) with 13V091, 13V117 or 07V063 (9 pigs/group). At 10 days post inoculation (dpi), 4 animals from each group were euthanized and tissues were collected for pathology, virological and serological analysis. 13V091 infection resulted in the highest respiratory disease scores and longest period of fever. Gross lung lesions were more pronounced for 13V091 (13%), than for 13V117 (7%) and 07V063 (11%). The nasal shedding and viremia was also most extensive with 13V091. The 13V091 group showed the highest virus replication in conchae, tonsils and retropharyngeal lymph nodes. 13V117 infection resulted in the lowest virus replication in lymphoid tissues. 13V091 showed higher numbers of sialoadhesin(-) infected cells/mm(2) in conchae, tonsils and spleen than 13V117 and 07V063. Neutralizing antibody response with 07V063 was stronger than with 13V091 and 13V117. It can be concluded that (i) 13V091 is a highly pathogenic type 1 subtype 1 PRRSV strain that replicates better than 07V063 and 13V117 and has a strong tropism for sialoadhesin(-) cells and (ii) despite the close genetic relationship between 13V117 and 07V063, 13V117 has an increased nasal replication and shedding, but a decreased replication in lymphoid tissues compared to 07V063.},
  articleno    = {37},
  author       = {Frydas, Ilias and Trus, Ivan and Kvisgaard, Lise and Bonckaert, Caroline and Avalakuppa Papi Reddy, Vishwanatha Reddy and Li, Yewei and Larsen, Lars and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {subtype 1,pathogenicity,type 1,07V063,13V117,PRRSV,13V091,MYSTERY SWINE DISEASE,LELYSTAD VIRUS,COMPARATIVE PATHOGENICITY,MYCOPLASMA-HYOPNEUMONIAE,EPIDEMIC ABORTION,INFECTION,PIGS,REPLICATION,CELL,CHALLENGE},
  language     = {eng},
  pages        = {17},
  title        = {Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) isolates},
  url          = {http://dx.doi.org/10.1186/s13567-015-0166-3},
  volume       = {46},
  year         = {2015},
}

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