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Development of bioelectrocatalytic activity stimulates mixed-culture reduction of glycerol in a bioelectrochemical system

(2015) MICROBIAL BIOTECHNOLOGY. 8(3). p.483-489
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Organization
Abstract
In a microbial bioelectrochemical system (BES), organic substrate such as glycerol can be reductively converted to 1,3-propanediol (1,3-PDO) by a mixed population biofilm growing on the cathode. Here, we show that 1,3-PDO yields positively correlated to the electrons supplied, increasing from 0.27 +/- 0.13 to 0.57 +/- 0.09mol PDOmol(-1) glycerol when the cathodic current switched from 1Am(-2) to 10Am(-2). Electrochemical measurements with linear sweep voltammetry (LSV) demonstrated that the biofilm was bioelectrocatalytically active and that the cathodic current was greatly enhanced only in the presence of both biofilm and glycerol, with an onset potential of -0.46V. This indicates that glycerol or its degradation products effectively served as cathodic electron acceptor. During long-term operation (>150 days), however, the yield decreased gradually to 0.13 +/- 0.02mol PDOmol(-1) glycerol, and the current-product correlation disappeared. The onset potentials for cathodic current decreased to -0.58V in the LSV tests at this stage, irrespective of the presence or absence of glycerol, with electrons from the cathode almost exclusively used for hydrogen evolution (accounted for 99.9% and 89.5% of the electrons transferred at glycerol and glycerol-free conditions respectively). Community analysis evidenced a decreasing relative abundance of Citrobacter in the biofilm, indicating a community succession leading to cathode independent processes relative to the glycerol. It is thus shown here that in processes where substrate conversion can occur independently of the electrode, electroactive microorganisms can be outcompeted and effectively disconnected from the substrate.
Keywords
GENERATION, CONVERSION, PERFORMANCE, ELECTRON-FLUXES, MICROBIAL FUEL-CELLS, ELECTRICITY, ELECTROSYNTHESIS, CARBON, FERMENTATION, COMMUNITIES

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Citation

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MLA
Zhou, Mi et al. “Development of Bioelectrocatalytic Activity Stimulates Mixed-culture Reduction of Glycerol in a Bioelectrochemical System.” MICROBIAL BIOTECHNOLOGY 8.3 (2015): 483–489. Print.
APA
Zhou, M., Freguia, S., Dennis, P. G., Keller, J., & Rabaey, K. (2015). Development of bioelectrocatalytic activity stimulates mixed-culture reduction of glycerol in a bioelectrochemical system. MICROBIAL BIOTECHNOLOGY, 8(3), 483–489.
Chicago author-date
Zhou, Mi, Stefano Freguia, Paul G Dennis, Jürg Keller, and Korneel Rabaey. 2015. “Development of Bioelectrocatalytic Activity Stimulates Mixed-culture Reduction of Glycerol in a Bioelectrochemical System.” Microbial Biotechnology 8 (3): 483–489.
Chicago author-date (all authors)
Zhou, Mi, Stefano Freguia, Paul G Dennis, Jürg Keller, and Korneel Rabaey. 2015. “Development of Bioelectrocatalytic Activity Stimulates Mixed-culture Reduction of Glycerol in a Bioelectrochemical System.” Microbial Biotechnology 8 (3): 483–489.
Vancouver
1.
Zhou M, Freguia S, Dennis PG, Keller J, Rabaey K. Development of bioelectrocatalytic activity stimulates mixed-culture reduction of glycerol in a bioelectrochemical system. MICROBIAL BIOTECHNOLOGY. 2015;8(3):483–9.
IEEE
[1]
M. Zhou, S. Freguia, P. G. Dennis, J. Keller, and K. Rabaey, “Development of bioelectrocatalytic activity stimulates mixed-culture reduction of glycerol in a bioelectrochemical system,” MICROBIAL BIOTECHNOLOGY, vol. 8, no. 3, pp. 483–489, 2015.
@article{5965422,
  abstract     = {In a microbial bioelectrochemical system (BES), organic substrate such as glycerol can be reductively converted to 1,3-propanediol (1,3-PDO) by a mixed population biofilm growing on the cathode. Here, we show that 1,3-PDO yields positively correlated to the electrons supplied, increasing from 0.27 +/- 0.13 to 0.57 +/- 0.09mol PDOmol(-1) glycerol when the cathodic current switched from 1Am(-2) to 10Am(-2). Electrochemical measurements with linear sweep voltammetry (LSV) demonstrated that the biofilm was bioelectrocatalytically active and that the cathodic current was greatly enhanced only in the presence of both biofilm and glycerol, with an onset potential of -0.46V. This indicates that glycerol or its degradation products effectively served as cathodic electron acceptor. During long-term operation (>150 days), however, the yield decreased gradually to 0.13 +/- 0.02mol PDOmol(-1) glycerol, and the current-product correlation disappeared. The onset potentials for cathodic current decreased to -0.58V in the LSV tests at this stage, irrespective of the presence or absence of glycerol, with electrons from the cathode almost exclusively used for hydrogen evolution (accounted for 99.9% and 89.5% of the electrons transferred at glycerol and glycerol-free conditions respectively). Community analysis evidenced a decreasing relative abundance of Citrobacter in the biofilm, indicating a community succession leading to cathode independent processes relative to the glycerol. It is thus shown here that in processes where substrate conversion can occur independently of the electrode, electroactive microorganisms can be outcompeted and effectively disconnected from the substrate.},
  author       = {Zhou, Mi and Freguia, Stefano and Dennis, Paul G and Keller, Jürg and Rabaey, Korneel},
  issn         = {1751-7907},
  journal      = {MICROBIAL BIOTECHNOLOGY},
  keywords     = {GENERATION,CONVERSION,PERFORMANCE,ELECTRON-FLUXES,MICROBIAL FUEL-CELLS,ELECTRICITY,ELECTROSYNTHESIS,CARBON,FERMENTATION,COMMUNITIES},
  language     = {eng},
  number       = {3},
  pages        = {483--489},
  title        = {Development of bioelectrocatalytic activity stimulates mixed-culture reduction of glycerol in a bioelectrochemical system},
  url          = {http://dx.doi.org/10.1111/1751-7915.12240},
  volume       = {8},
  year         = {2015},
}

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