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Microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules?

Tine De Ryck (UGent) , Eline Vanlancker (UGent) , Charlotte Grootaert (UGent) , Bart Roman (UGent) , Laurens De Coen (UGent) , Isabel Vandenberghe (UGent) , Christian Stevens (UGent) , Marc Bracke (UGent) , Tom Van de Wiele (UGent) and Barbara Vanhoecke (UGent)
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Abstract
Awareness of the impact of microbiota in both health and disease is growing. Using a new in vitro oral mucosa co-culture model, we recently showed a clear inhibition of epithelial wound healing in the presence of an oral microbial community. In this paper, we have used the same model in combination with specific oral microbial species to obtain a better insight into the role of the oral microbiota in wound healing. Monocultures of Klebsiella oxytoca and Lactobacillus salivarius significantly inhibited wound healing with similar to 20%, whereas Streptococcus mitis and S. oralis enhanced the healing process with similar to 15% in 24 h. Yet, neither S. oralis or S. mitis were able to counteract the inhibitory effects from K. oxytoca on wound healing. Other tested microbial species had no effect on wound healing. Apart from this species-dependency, the inhibitory effect on wound healing depended on a microbial threshold concentration. Further mechanistic experiments with K. oxytoca excluded different microbial factors and hypothesized that quorum sensing molecules might play a role in the inter-kingdom signalling during wound healing. These results are important for the development of new strategies for the management of (infected) wounds and ulcerations.
Keywords
Epithelial wound healing, Co-culture model, Klebsiella oxytoca, Quorum sensing, Monocultures, HOMOSERINE LACTONE, IN-VITRO, BACTERIA, CELLS, REPAIR, KLEBSIELLA, MIGRATION, GLUCOSE, MUCOSA

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Chicago
De Ryck, Tine, Eline Vanlancker, Charlotte Grootaert, Bart Roman, Laurens De Coen, Isabel Vandenberghe, Christian Stevens, Marc Bracke, Tom Van de Wiele, and Barbara Vanhoecke. 2015. “Microbial Inhibition of Oral Epithelial Wound Recovery: Potential Role for Quorum Sensing Molecules?” Amb Express 5.
APA
De Ryck, T., Vanlancker, E., Grootaert, C., Roman, B., De Coen, L., Vandenberghe, I., Stevens, C., et al. (2015). Microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules? AMB EXPRESS, 5.
Vancouver
1.
De Ryck T, Vanlancker E, Grootaert C, Roman B, De Coen L, Vandenberghe I, et al. Microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules? AMB EXPRESS. 2015;5.
MLA
De Ryck, Tine, Eline Vanlancker, Charlotte Grootaert, et al. “Microbial Inhibition of Oral Epithelial Wound Recovery: Potential Role for Quorum Sensing Molecules?” AMB EXPRESS 5 (2015): n. pag. Print.
@article{5965184,
  abstract     = {Awareness of the impact of microbiota in both health and disease is growing. Using a new in vitro oral mucosa co-culture model, we recently showed a clear inhibition of epithelial wound healing in the presence of an oral microbial community. In this paper, we have used the same model in combination with specific oral microbial species to obtain a better insight into the role of the oral microbiota in wound healing. Monocultures of Klebsiella oxytoca and Lactobacillus salivarius significantly inhibited wound healing with similar to 20\%, whereas Streptococcus mitis and S. oralis enhanced the healing process with similar to 15\% in 24 h. Yet, neither S. oralis or S. mitis were able to counteract the inhibitory effects from K. oxytoca on wound healing. Other tested microbial species had no effect on wound healing. Apart from this species-dependency, the inhibitory effect on wound healing depended on a microbial threshold concentration. Further mechanistic experiments with K. oxytoca excluded different microbial factors and hypothesized that quorum sensing molecules might play a role in the inter-kingdom signalling during wound healing. These results are important for the development of new strategies for the management of (infected) wounds and ulcerations.},
  articleno    = {27},
  author       = {De Ryck, Tine and Vanlancker, Eline and Grootaert, Charlotte and Roman, Bart and De Coen, Laurens and Vandenberghe, Isabel and Stevens, Christian and Bracke, Marc and Van de Wiele, Tom and Vanhoecke, Barbara},
  issn         = {2191-0855},
  journal      = {AMB EXPRESS},
  language     = {eng},
  pages        = {12},
  title        = {Microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules?},
  url          = {http://dx.doi.org/10.1186/s13568-015-0116-5},
  volume       = {5},
  year         = {2015},
}

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