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Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry : a new approach for the diagnosis and prognosis of liver cirrhosis?

Marta Costas Rodriguez (UGent) , Yulia Anoshkina (UGent) , Sara Lauwens (UGent) , Hans Van Vlierberghe (UGent) , Joris Delanghe (UGent) and Frank Vanhaecke (UGent)
(2015) METALLOMICS. 7(3). p.491-498
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Abstract
The isotopic composition of blood serum Cu has been investigated as a potential parameter for the diagnosis and prognosis of liver cirrhosis. Serum samples from supposedly healthy women (reference population) and from a group of female patients suffering from liver cirrhosis of different etiologies were analysed. The procedure for isolation of serum Cu and the measurement protocol for its isotopic analysis by multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS) were evaluated. Significant differences in the isotopic composition of Cu were observed between the reference population and the patients. A wide spread in delta Cu-65 was observed within the cirrhosis population and delta Cu-65 seems to be linked to the severity of the disease. Patients with end-stage liver disease showed a significantly lighter serum Cu isotopic composition. Many clinical parameters used for the diagnosis and monitoring of liver diseases, i.e. the levels of aspartate aminotransferase, De Ritis ratio, prothrombin and international normalized ratio, albumin, bilirubin, Na and C-reactive protein, correlate well with the delta Cu-65 values, as did the ceruloplasmin level and the ceruloplasmin/Cu concentration ratio. The isotopic composition of serum Cu appears to reveal the synthetic and hepatocellular function of the liver synergistically with inflammation and fluid retention in the cohort studied. A relevant relationship was also observed between delta Cu-65 and scores of mortality risk, such as the Model for End-stage Liver Disease (MELD) and MELD-Na. Thus, the isotopic composition of serum Cu shows potential as a new approach for the prognosis of liver disease, and although further investigation is required, for evaluation of the mortality risk in end-stage liver disease and prioritization of liver transplants
Keywords
CERULOPLASMIN, SODIUM, COPPER, OXIDATIVE STRESS, PRIMARY BILIARY-CIRRHOSIS, MELD, TOXICITY, STANDARD, ASCITES, DISEASE

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MLA
Costas Rodriguez, Marta, et al. “Isotopic Analysis of Cu in Blood Serum by Multi-Collector ICP-Mass Spectrometry : A New Approach for the Diagnosis and Prognosis of Liver Cirrhosis?” METALLOMICS, vol. 7, no. 3, 2015, pp. 491–98, doi:10.1039/c4mt00319e.
APA
Costas Rodriguez, M., Anoshkina, Y., Lauwens, S., Van Vlierberghe, H., Delanghe, J., & Vanhaecke, F. (2015). Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry : a new approach for the diagnosis and prognosis of liver cirrhosis? METALLOMICS, 7(3), 491–498. https://doi.org/10.1039/c4mt00319e
Chicago author-date
Costas Rodriguez, Marta, Yulia Anoshkina, Sara Lauwens, Hans Van Vlierberghe, Joris Delanghe, and Frank Vanhaecke. 2015. “Isotopic Analysis of Cu in Blood Serum by Multi-Collector ICP-Mass Spectrometry : A New Approach for the Diagnosis and Prognosis of Liver Cirrhosis?” METALLOMICS 7 (3): 491–98. https://doi.org/10.1039/c4mt00319e.
Chicago author-date (all authors)
Costas Rodriguez, Marta, Yulia Anoshkina, Sara Lauwens, Hans Van Vlierberghe, Joris Delanghe, and Frank Vanhaecke. 2015. “Isotopic Analysis of Cu in Blood Serum by Multi-Collector ICP-Mass Spectrometry : A New Approach for the Diagnosis and Prognosis of Liver Cirrhosis?” METALLOMICS 7 (3): 491–498. doi:10.1039/c4mt00319e.
Vancouver
1.
Costas Rodriguez M, Anoshkina Y, Lauwens S, Van Vlierberghe H, Delanghe J, Vanhaecke F. Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry : a new approach for the diagnosis and prognosis of liver cirrhosis? METALLOMICS. 2015;7(3):491–8.
IEEE
[1]
M. Costas Rodriguez, Y. Anoshkina, S. Lauwens, H. Van Vlierberghe, J. Delanghe, and F. Vanhaecke, “Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry : a new approach for the diagnosis and prognosis of liver cirrhosis?,” METALLOMICS, vol. 7, no. 3, pp. 491–498, 2015.
@article{5963189,
  abstract     = {{The isotopic composition of blood serum Cu has been investigated as a potential parameter for the diagnosis and prognosis of liver cirrhosis. Serum samples from supposedly healthy women (reference population) and from a group of female patients suffering from liver cirrhosis of different etiologies were analysed. The procedure for isolation of serum Cu and the measurement protocol for its isotopic analysis by multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS) were evaluated. Significant differences in the isotopic composition of Cu were observed between the reference population and the patients. A wide spread in delta Cu-65 was observed within the cirrhosis population and delta Cu-65 seems to be linked to the severity of the disease. Patients with end-stage liver disease showed a significantly lighter serum Cu isotopic composition. Many clinical parameters used for the diagnosis and monitoring of liver diseases, i.e. the levels of aspartate aminotransferase, De Ritis ratio, prothrombin and international normalized ratio, albumin, bilirubin, Na and C-reactive protein, correlate well with the delta Cu-65 values, as did the ceruloplasmin level and the ceruloplasmin/Cu concentration ratio. The isotopic composition of serum Cu appears to reveal the synthetic and hepatocellular function of the liver synergistically with inflammation and fluid retention in the cohort studied. A relevant relationship was also observed between delta Cu-65 and scores of mortality risk, such as the Model for End-stage Liver Disease (MELD) and MELD-Na. Thus, the isotopic composition of serum Cu shows potential as a new approach for the prognosis of liver disease, and although further investigation is required, for evaluation of the mortality risk in end-stage liver disease and prioritization of liver transplants}},
  author       = {{Costas Rodriguez, Marta and Anoshkina, Yulia and Lauwens, Sara and Van Vlierberghe, Hans and Delanghe, Joris and Vanhaecke, Frank}},
  issn         = {{1756-5901}},
  journal      = {{METALLOMICS}},
  keywords     = {{CERULOPLASMIN,SODIUM,COPPER,OXIDATIVE STRESS,PRIMARY BILIARY-CIRRHOSIS,MELD,TOXICITY,STANDARD,ASCITES,DISEASE}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{491--498}},
  title        = {{Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry : a new approach for the diagnosis and prognosis of liver cirrhosis?}},
  url          = {{http://doi.org/10.1039/c4mt00319e}},
  volume       = {{7}},
  year         = {{2015}},
}

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