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In vitro polyploidisation of Helleborus species

Emmy Dhooghe UGent, Wim Grunewald UGent, Leen Leus and Marie-Christine Van Labeke UGent (2009) EUPHYTICA. 165(1). p.89-95
abstract
Helleborus species are members of the family of the Ranunculaceae. These popular perennials are all diploids (2n = 2x = 32). This study investigates polyploidy induction by different antimitotic agents. Colchicine, oryzalin and trifluralin were tested in vitro on shoots of Helleborus niger, H. orientalis and H. 9 nigercors. Furthermore the effect of the antimitotic agents on the viability and the multiplication rate of cultured plantlets were analyzed. Flow cytometry demonstrated that polyploidisation was genotype dependent: using H. niger, tetraploids were obtained using either oryzalin (3 mu M) or trifluralin (3 or 10 lM), whereas for H. 9 nigercors only trifluralin (3 or 10 mu M) induced polyploidisation. For H. orientalis neither treatment was effective to produce tetraploids or mixoploids. For these three species, colchicine (100 mu M) was ineffective. The polyploidisation events in H. niger and H. 9 nigercors were confirmed by chromosome counts of mounted nuclei derived from root tips (2n = 4x = 64).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Flow cytometry, Colchicine, Helleborus, Oryzalin, Polyploidisation, Ranunculaceae, Trifluralin, DINITROANILINE HERBICIDE, MISCANTHUS-SINENSIS, PLANT MORPHOLOGY, COLCHICINE, ORYZALIN, INDUCTION, ROSA, EVOLUTION, TETRAPLOIDS, TUBULIN
journal title
EUPHYTICA
Euphytica
volume
165
issue
1
pages
89 - 95
Web of Science type
Article
Web of Science id
000262086900009
JCR category
HORTICULTURE
JCR impact factor
1.405 (2009)
JCR rank
7/30 (2009)
JCR quartile
1 (2009)
ISSN
0014-2336
DOI
10.1007/s10681-008-9763-9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
596012
handle
http://hdl.handle.net/1854/LU-596012
date created
2009-04-21 09:13:48
date last changed
2009-09-07 13:19:31
@article{596012,
  abstract     = {Helleborus species are members of the family of the Ranunculaceae. These popular perennials are all diploids (2n = 2x = 32). This study investigates polyploidy induction by different antimitotic agents. Colchicine, oryzalin and trifluralin were tested in vitro on shoots of Helleborus niger, H. orientalis and H. 9 nigercors. Furthermore the effect of the antimitotic agents on the viability and the multiplication rate of cultured plantlets were analyzed. Flow cytometry demonstrated that polyploidisation was genotype dependent: using H. niger, tetraploids were obtained using either oryzalin (3 mu M) or trifluralin (3 or 10 lM), whereas for H. 9 nigercors only trifluralin (3 or 10 mu M) induced polyploidisation. For H. orientalis neither treatment was effective to produce tetraploids or mixoploids. For these three species, colchicine (100 mu M) was ineffective. The polyploidisation events in H. niger and H. 9 nigercors were confirmed by chromosome counts of mounted nuclei derived from root tips (2n = 4x = 64).},
  author       = {Dhooghe, Emmy and Grunewald, Wim and Leus, Leen and Van Labeke, Marie-Christine},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {Flow cytometry,Colchicine,Helleborus,Oryzalin,Polyploidisation,Ranunculaceae,Trifluralin,DINITROANILINE HERBICIDE,MISCANTHUS-SINENSIS,PLANT MORPHOLOGY,COLCHICINE,ORYZALIN,INDUCTION,ROSA,EVOLUTION,TETRAPLOIDS,TUBULIN},
  language     = {eng},
  number       = {1},
  pages        = {89--95},
  title        = {In vitro polyploidisation of Helleborus species},
  url          = {http://dx.doi.org/10.1007/s10681-008-9763-9},
  volume       = {165},
  year         = {2009},
}

Chicago
Dhooghe, Emmy, Wim Grunewald, Leen Leus, and Marie-Christine Van Labeke. 2009. “In Vitro Polyploidisation of Helleborus Species.” Euphytica 165 (1): 89–95.
APA
Dhooghe, E., Grunewald, W., Leus, L., & Van Labeke, M.-C. (2009). In vitro polyploidisation of Helleborus species. EUPHYTICA, 165(1), 89–95.
Vancouver
1.
Dhooghe E, Grunewald W, Leus L, Van Labeke M-C. In vitro polyploidisation of Helleborus species. EUPHYTICA. 2009;165(1):89–95.
MLA
Dhooghe, Emmy, Wim Grunewald, Leen Leus, et al. “In Vitro Polyploidisation of Helleborus Species.” EUPHYTICA 165.1 (2009): 89–95. Print.