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In vitro polyploidisation of Helleborus species

Emmy Dhooghe (UGent) , Wim Grunewald (UGent) , Leen Leus and Marie-Christine Van Labeke (UGent)
(2009) EUPHYTICA. 165(1). p.89-95
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Abstract
Helleborus species are members of the family of the Ranunculaceae. These popular perennials are all diploids (2n = 2x = 32). This study investigates polyploidy induction by different antimitotic agents. Colchicine, oryzalin and trifluralin were tested in vitro on shoots of Helleborus niger, H. orientalis and H. 9 nigercors. Furthermore the effect of the antimitotic agents on the viability and the multiplication rate of cultured plantlets were analyzed. Flow cytometry demonstrated that polyploidisation was genotype dependent: using H. niger, tetraploids were obtained using either oryzalin (3 mu M) or trifluralin (3 or 10 lM), whereas for H. 9 nigercors only trifluralin (3 or 10 mu M) induced polyploidisation. For H. orientalis neither treatment was effective to produce tetraploids or mixoploids. For these three species, colchicine (100 mu M) was ineffective. The polyploidisation events in H. niger and H. 9 nigercors were confirmed by chromosome counts of mounted nuclei derived from root tips (2n = 4x = 64).
Keywords
Flow cytometry, Colchicine, Helleborus, Oryzalin, Polyploidisation, Ranunculaceae, Trifluralin, DINITROANILINE HERBICIDE, MISCANTHUS-SINENSIS, PLANT MORPHOLOGY, COLCHICINE, ORYZALIN, INDUCTION, ROSA, EVOLUTION, TETRAPLOIDS, TUBULIN

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MLA
Dhooghe, Emmy, et al. “In Vitro Polyploidisation of Helleborus Species.” EUPHYTICA, vol. 165, no. 1, 2009, pp. 89–95, doi:10.1007/s10681-008-9763-9.
APA
Dhooghe, E., Grunewald, W., Leus, L., & Van Labeke, M.-C. (2009). In vitro polyploidisation of Helleborus species. EUPHYTICA, 165(1), 89–95. https://doi.org/10.1007/s10681-008-9763-9
Chicago author-date
Dhooghe, Emmy, Wim Grunewald, Leen Leus, and Marie-Christine Van Labeke. 2009. “In Vitro Polyploidisation of Helleborus Species.” EUPHYTICA 165 (1): 89–95. https://doi.org/10.1007/s10681-008-9763-9.
Chicago author-date (all authors)
Dhooghe, Emmy, Wim Grunewald, Leen Leus, and Marie-Christine Van Labeke. 2009. “In Vitro Polyploidisation of Helleborus Species.” EUPHYTICA 165 (1): 89–95. doi:10.1007/s10681-008-9763-9.
Vancouver
1.
Dhooghe E, Grunewald W, Leus L, Van Labeke M-C. In vitro polyploidisation of Helleborus species. EUPHYTICA. 2009;165(1):89–95.
IEEE
[1]
E. Dhooghe, W. Grunewald, L. Leus, and M.-C. Van Labeke, “In vitro polyploidisation of Helleborus species,” EUPHYTICA, vol. 165, no. 1, pp. 89–95, 2009.
@article{596012,
  abstract     = {{Helleborus species are members of the family of the Ranunculaceae. These popular perennials are all diploids (2n = 2x = 32). This study investigates polyploidy induction by different antimitotic agents. Colchicine, oryzalin and trifluralin were tested in vitro on shoots of Helleborus niger, H. orientalis and H. 9 nigercors. Furthermore the effect of the antimitotic agents on the viability and the multiplication rate of cultured plantlets were analyzed. Flow cytometry demonstrated that polyploidisation was genotype dependent: using H. niger, tetraploids were obtained using either oryzalin (3 mu M) or trifluralin (3 or 10 lM), whereas for H. 9 nigercors only trifluralin (3 or 10 mu M) induced polyploidisation. For H. orientalis neither treatment was effective to produce tetraploids or mixoploids. For these three species, colchicine (100 mu M) was ineffective. The polyploidisation events in H. niger and H. 9 nigercors were confirmed by chromosome counts of mounted nuclei derived from root tips (2n = 4x = 64).}},
  author       = {{Dhooghe, Emmy and Grunewald, Wim and Leus, Leen and Van Labeke, Marie-Christine}},
  issn         = {{0014-2336}},
  journal      = {{EUPHYTICA}},
  keywords     = {{Flow cytometry,Colchicine,Helleborus,Oryzalin,Polyploidisation,Ranunculaceae,Trifluralin,DINITROANILINE HERBICIDE,MISCANTHUS-SINENSIS,PLANT MORPHOLOGY,COLCHICINE,ORYZALIN,INDUCTION,ROSA,EVOLUTION,TETRAPLOIDS,TUBULIN}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{89--95}},
  title        = {{In vitro polyploidisation of Helleborus species}},
  url          = {{http://doi.org/10.1007/s10681-008-9763-9}},
  volume       = {{165}},
  year         = {{2009}},
}
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