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Understanding Thiel embalming in pig kidneys to develop a new circulation model

Wouter Willaert, Marie De Vos, Tom Van Hoof UGent, Louke Delrue, Piet Pattyn UGent and Katharina D'Herde UGent (2015) PLOS ONE. 10(3).
abstract
The quality of tissue preservation in Thiel embalmed bodiesvaries. Research on the administered embalming volume and its vascular distribution may elucidate one of the mechanisms of tissue preservation and allow for new applications of Thiel embalming. Vascular embalming with (group 1, n = 15) or without (group 2, n = 20) contrast agent was initiated in pig kidneys. The distribution of Thiel embalming solution in group 1 was visualized using computed tomography. The kidneys in both groups were then immersed in concentrated salt solutions to reduce their weight and volume. Afterwards, to mimic a lifelike circulation in the vessels, group 2 underwent pump-driven reperfusion for 120 minutes with either paraffinum perliquidum or diluted polyethylene glycol. The circulation was imaged with computed tomography. All of the kidneys were adequately preserved. The embalming solution spread diffusely in the kidney, but fluid accumulation was present. Subsequent immersion in concentrated salt solutions reduced weight (P < 0.01) and volume (P < 0.01). Reperfusion for 120 minutes was established in group 2. Paraffinum perliquidum filled both major vessels and renal tissue, whereas diluted polyethylene glycol spread widely in the kidney. There were no increases in weight (P = 0.26) and volume (P = 0.79); and pressure further decreased (P = 0.032) after more than 60 minutes of reperfusion with paraffinum perliquidum, whereas there were increases in weight (P = 0.005), volume (P = 0.032) and pressure (P < 0.0001) after reperfusion with diluted polyethylene glycol. Arterial embalming of kidneys results in successful preservation due to complete parenchymatous spreading. More research is needed to determine whether other factors affect embalming quality. Dehydration is an effective method to regain the organs' initial status. Prolonged vascular reperfusion with paraffinum perliquidum can be established in this model without increases in weight, volume and pressure.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PRESERVATION, HUMAN CADAVERS, FORMALIN, ANATOMY
journal title
PLOS ONE
PLoS One
volume
10
issue
3
article number
e0120114
pages
16 pages
Web of Science type
Article
Web of Science id
000351880000067
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.057 (2015)
JCR rank
11/63 (2015)
JCR quartile
1 (2015)
ISSN
1932-6203
DOI
10.1371/journal.pone.0120114
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5959504
handle
http://hdl.handle.net/1854/LU-5959504
date created
2015-05-19 13:40:43
date last changed
2016-12-21 15:42:50
@article{5959504,
  abstract     = {The quality of tissue preservation in Thiel embalmed bodiesvaries. Research on the administered embalming volume and its vascular distribution may elucidate one of the mechanisms of tissue preservation and allow for new applications of Thiel embalming. Vascular embalming with (group 1, n = 15) or without (group 2, n = 20) contrast agent was initiated in pig kidneys. The distribution of Thiel embalming solution in group 1 was visualized using computed tomography. The kidneys in both groups were then immersed in concentrated salt solutions to reduce their weight and volume. Afterwards, to mimic a lifelike circulation in the vessels, group 2 underwent pump-driven reperfusion for 120 minutes with either paraffinum perliquidum or diluted polyethylene glycol. The circulation was imaged with computed tomography. All of the kidneys were adequately preserved. The embalming solution spread diffusely in the kidney, but fluid accumulation was present. Subsequent immersion in concentrated salt solutions reduced weight (P {\textlangle} 0.01) and volume (P {\textlangle} 0.01). Reperfusion for 120 minutes was established in group 2. Paraffinum perliquidum filled both major vessels and renal tissue, whereas diluted polyethylene glycol spread widely in the kidney. There were no increases in weight (P = 0.26) and volume (P = 0.79); and pressure further decreased (P = 0.032) after more than 60 minutes of reperfusion with paraffinum perliquidum, whereas there were increases in weight (P = 0.005), volume (P = 0.032) and pressure (P {\textlangle} 0.0001) after reperfusion with diluted polyethylene glycol. Arterial embalming of kidneys results in successful preservation due to complete parenchymatous spreading. More research is needed to determine whether other factors affect embalming quality. Dehydration is an effective method to regain the organs' initial status. Prolonged vascular reperfusion with paraffinum perliquidum can be established in this model without increases in weight, volume and pressure.},
  articleno    = {e0120114},
  author       = {Willaert, Wouter and De Vos, Marie and Van Hoof, Tom and Delrue, Louke and Pattyn, Piet and D'Herde, Katharina},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {PRESERVATION,HUMAN CADAVERS,FORMALIN,ANATOMY},
  language     = {eng},
  number       = {3},
  pages        = {16},
  title        = {Understanding Thiel embalming in pig kidneys to develop a new circulation model},
  url          = {http://dx.doi.org/10.1371/journal.pone.0120114},
  volume       = {10},
  year         = {2015},
}

Chicago
WILLAERT, WOUTER, Marie De Vos, Tom Van Hoof, LOUKE DELRUE, Piet Pattyn, and Katharina D’Herde. 2015. “Understanding Thiel Embalming in Pig Kidneys to Develop a New Circulation Model.” Plos One 10 (3).
APA
WILLAERT, W., De Vos, M., Van Hoof, T., DELRUE, L., Pattyn, P., & D’Herde, K. (2015). Understanding Thiel embalming in pig kidneys to develop a new circulation model. PLOS ONE, 10(3).
Vancouver
1.
WILLAERT W, De Vos M, Van Hoof T, DELRUE L, Pattyn P, D’Herde K. Understanding Thiel embalming in pig kidneys to develop a new circulation model. PLOS ONE. 2015;10(3).
MLA
WILLAERT, WOUTER, Marie De Vos, Tom Van Hoof, et al. “Understanding Thiel Embalming in Pig Kidneys to Develop a New Circulation Model.” PLOS ONE 10.3 (2015): n. pag. Print.