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Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR

Sarah Tavernier (UGent) and Tom Coenye (UGent)
(2015) PEERJ. 3.
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Abstract
Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.
Keywords
PMA-qPCR, CYSTIC-FIBROSIS PATIENTS, Resistance, Pseudomonas aeruginosa, Biofilm, PREFERENTIAL DETECTION, PROPIDIUM MONOAZIDE, AMPLIFICATION, COMBINATION, TOBRAMYCIN, INFECTION, EFFICACY, DNA

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Citation

Please use this url to cite or link to this publication:

Chicago
Tavernier, Sarah, and Tom Coenye. 2015. “Quantification of Pseudomonas Aeruginosa in Multispecies Biofilms Using PMA-qPCR.” Peerj 3.
APA
Tavernier, Sarah, & Coenye, T. (2015). Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR. PEERJ, 3.
Vancouver
1.
Tavernier S, Coenye T. Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR. PEERJ. 2015;3.
MLA
Tavernier, Sarah, and Tom Coenye. “Quantification of Pseudomonas Aeruginosa in Multispecies Biofilms Using PMA-qPCR.” PEERJ 3 (2015): n. pag. Print.
@article{5957388,
  abstract     = {Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.},
  articleno    = {e787},
  author       = {Tavernier, Sarah and Coenye, Tom},
  issn         = {2167-8359},
  journal      = {PEERJ},
  keyword      = {PMA-qPCR,CYSTIC-FIBROSIS PATIENTS,Resistance,Pseudomonas aeruginosa,Biofilm,PREFERENTIAL DETECTION,PROPIDIUM MONOAZIDE,AMPLIFICATION,COMBINATION,TOBRAMYCIN,INFECTION,EFFICACY,DNA},
  language     = {eng},
  pages        = {15},
  title        = {Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR},
  url          = {http://dx.doi.org/10.7717/peerj.787},
  volume       = {3},
  year         = {2015},
}

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