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Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis

(2009) JOURNAL OF PROTEOME RESEARCH. 8(2). p.463-470
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Abstract
The hepatic histology in nonalcoholic fatty liver disease can vary from isolated hepatic steatosis to steatohepatitis can progress to cirrhosis and liver-related death, The aim was to evaluate the use of blood serum N-glycan fingerprinting as a tool for differential diagnosis of nonalcoholic steatohepatitis from steatosis. A group of 47 patients with NAFLD was diagnosed by clinical laboratory analysis and ultrasonography, and was studied histologically using the Brunt's scoring system. The control group included 13 healthy individuals. N-glycan profiles of serum proteins were determined by DNA sequencer-based carbohydrate analytical profiling. We have found that the concentrations of two glycans (NGA2F and NA2) and their logarithm ratio of NGA2F versus NA2 (named GlycoNashTest) were associated with the degree of NASH-related fibrosis, but had no correlation with the grade of inflammation nor steatosis severity. When used to screen NAFLD patients, GlycoNashTest could identify advanced NASH-related fibrosis (F3-F4) with the diagnosis sensitivity of 89.5% and specificity of 71.4%. The serum N-glycan profile is a promising noninvasive method for detecting NASH or NASH-related fibrosis in NAFLD patients, which could be a valuable supplement to other markers currently used in diagnosis of NASH.
Keywords
NAFLD, NASH, liver fibrosis, noninvasive, fatty liver, N-glycan, glycomics, FATTY LIVER-DISEASE, HEPATOCELLULAR-CARCINOMA, CORE FUCOSYLATION, GLYCOMIC CHANGES, CIRRHOSIS, HEPATITIS, DIAGNOSIS, ACETYLGLUCOSAMINYLTRANSFERASE, EXPRESSION, ANTIBODY

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Citation

Please use this url to cite or link to this publication:

Chicago
Chen, Cuiying, Hemda Schmilovtz-Weiss, Xue-en Liu, Orit Pappo, Marisa Halpern, Jacqueline Sulkes, Marius Braun, et al. 2009. “Serum Protein N-glycans Profiling for the Discovery of Potential Biomarkers for Nonalcoholic Steatohepatitis.” Journal of Proteome Research 8 (2): 463–470.
APA
Chen, Cuiying, Schmilovtz-Weiss, H., Liu, X., Pappo, O., Halpern, M., Sulkes, J., Braun, M., et al. (2009). Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis. JOURNAL OF PROTEOME RESEARCH, 8(2), 463–470.
Vancouver
1.
Chen C, Schmilovtz-Weiss H, Liu X, Pappo O, Halpern M, Sulkes J, et al. Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis. JOURNAL OF PROTEOME RESEARCH. 2009;8(2):463–70.
MLA
Chen, Cuiying, Hemda Schmilovtz-Weiss, Xue-en Liu, et al. “Serum Protein N-glycans Profiling for the Discovery of Potential Biomarkers for Nonalcoholic Steatohepatitis.” JOURNAL OF PROTEOME RESEARCH 8.2 (2009): 463–470. Print.
@article{595677,
  abstract     = {The hepatic histology in nonalcoholic fatty liver disease can vary from isolated hepatic steatosis to steatohepatitis can progress to cirrhosis and liver-related death, The aim was to evaluate the use of blood serum N-glycan fingerprinting as a tool for differential diagnosis of nonalcoholic steatohepatitis from steatosis. A group of 47 patients with NAFLD was diagnosed by clinical laboratory analysis and ultrasonography, and was studied histologically using the Brunt's scoring system. The control group included 13 healthy individuals. N-glycan profiles of serum proteins were determined by DNA sequencer-based carbohydrate analytical profiling. We have found that the concentrations of two glycans (NGA2F and NA2) and their logarithm ratio of NGA2F versus NA2 (named GlycoNashTest) were associated with the degree of NASH-related fibrosis, but had no correlation with the grade of inflammation nor steatosis severity. When used to screen NAFLD patients, GlycoNashTest could identify advanced NASH-related fibrosis (F3-F4) with the diagnosis sensitivity of 89.5\% and specificity of 71.4\%. The serum N-glycan profile is a promising noninvasive method for detecting NASH or NASH-related fibrosis in NAFLD patients, which could be a valuable supplement to other markers currently used in diagnosis of NASH.},
  author       = {Chen, Cuiying and Schmilovtz-Weiss, Hemda and Liu, Xue-en and Pappo, Orit and Halpern, Marisa and Sulkes, Jacqueline and Braun, Marius and Cohen, Maya and Barak, Nir and Tur-Kaspa, Ran and Vanhooren, Valerie and Van Vlierberghe, Hans and Libert, Claude and Contreras, Roland and Ben-Arl, Ziv},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  language     = {eng},
  number       = {2},
  pages        = {463--470},
  title        = {Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis},
  url          = {http://dx.doi.org/10.1021/pr800656e},
  volume       = {8},
  year         = {2009},
}

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