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Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis

Cuiying Chen UGent, Hemda Schmilovtz-Weiss, Xue-en Liu, Orit Pappo, Marisa Halpern, Jacqueline Sulkes, Marius Braun, Maya Cohen, Nir Barak and Ran Tur-Kaspa, et al. (2009) JOURNAL OF PROTEOME RESEARCH. 8(2). p.463-470
abstract
The hepatic histology in nonalcoholic fatty liver disease can vary from isolated hepatic steatosis to steatohepatitis can progress to cirrhosis and liver-related death, The aim was to evaluate the use of blood serum N-glycan fingerprinting as a tool for differential diagnosis of nonalcoholic steatohepatitis from steatosis. A group of 47 patients with NAFLD was diagnosed by clinical laboratory analysis and ultrasonography, and was studied histologically using the Brunt's scoring system. The control group included 13 healthy individuals. N-glycan profiles of serum proteins were determined by DNA sequencer-based carbohydrate analytical profiling. We have found that the concentrations of two glycans (NGA2F and NA2) and their logarithm ratio of NGA2F versus NA2 (named GlycoNashTest) were associated with the degree of NASH-related fibrosis, but had no correlation with the grade of inflammation nor steatosis severity. When used to screen NAFLD patients, GlycoNashTest could identify advanced NASH-related fibrosis (F3-F4) with the diagnosis sensitivity of 89.5% and specificity of 71.4%. The serum N-glycan profile is a promising noninvasive method for detecting NASH or NASH-related fibrosis in NAFLD patients, which could be a valuable supplement to other markers currently used in diagnosis of NASH.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NAFLD, NASH, liver fibrosis, noninvasive, fatty liver, N-glycan, glycomics, FATTY LIVER-DISEASE, HEPATOCELLULAR-CARCINOMA, CORE FUCOSYLATION, GLYCOMIC CHANGES, CIRRHOSIS, HEPATITIS, DIAGNOSIS, ACETYLGLUCOSAMINYLTRANSFERASE, EXPRESSION, ANTIBODY
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
8
issue
2
pages
463 - 470
Web of Science type
Article
Web of Science id
000263193300007
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.132 (2009)
JCR rank
8/65 (2009)
JCR quartile
1 (2009)
ISSN
1535-3893
DOI
10.1021/pr800656e
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
595677
handle
http://hdl.handle.net/1854/LU-595677
date created
2009-04-20 13:11:51
date last changed
2012-06-26 14:31:41
@article{595677,
  abstract     = {The hepatic histology in nonalcoholic fatty liver disease can vary from isolated hepatic steatosis to steatohepatitis can progress to cirrhosis and liver-related death, The aim was to evaluate the use of blood serum N-glycan fingerprinting as a tool for differential diagnosis of nonalcoholic steatohepatitis from steatosis. A group of 47 patients with NAFLD was diagnosed by clinical laboratory analysis and ultrasonography, and was studied histologically using the Brunt's scoring system. The control group included 13 healthy individuals. N-glycan profiles of serum proteins were determined by DNA sequencer-based carbohydrate analytical profiling. We have found that the concentrations of two glycans (NGA2F and NA2) and their logarithm ratio of NGA2F versus NA2 (named GlycoNashTest) were associated with the degree of NASH-related fibrosis, but had no correlation with the grade of inflammation nor steatosis severity. When used to screen NAFLD patients, GlycoNashTest could identify advanced NASH-related fibrosis (F3-F4) with the diagnosis sensitivity of 89.5\% and specificity of 71.4\%. The serum N-glycan profile is a promising noninvasive method for detecting NASH or NASH-related fibrosis in NAFLD patients, which could be a valuable supplement to other markers currently used in diagnosis of NASH.},
  author       = {Chen, Cuiying and Schmilovtz-Weiss, Hemda and Liu, Xue-en and Pappo, Orit and Halpern, Marisa and Sulkes, Jacqueline and Braun, Marius and Cohen, Maya and Barak, Nir and Tur-Kaspa, Ran and Vanhooren, Valerie and Van Vlierberghe, Hans and Libert, Claude and Contreras, Roland and Ben-Arl, Ziv},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {NAFLD,NASH,liver fibrosis,noninvasive,fatty liver,N-glycan,glycomics,FATTY LIVER-DISEASE,HEPATOCELLULAR-CARCINOMA,CORE FUCOSYLATION,GLYCOMIC CHANGES,CIRRHOSIS,HEPATITIS,DIAGNOSIS,ACETYLGLUCOSAMINYLTRANSFERASE,EXPRESSION,ANTIBODY},
  language     = {eng},
  number       = {2},
  pages        = {463--470},
  title        = {Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis},
  url          = {http://dx.doi.org/10.1021/pr800656e},
  volume       = {8},
  year         = {2009},
}

Chicago
Chen, Cuiying, Hemda Schmilovtz-Weiss, Xue-en Liu, Orit Pappo, Marisa Halpern, Jacqueline Sulkes, Marius Braun, et al. 2009. “Serum Protein N-glycans Profiling for the Discovery of Potential Biomarkers for Nonalcoholic Steatohepatitis.” Journal of Proteome Research 8 (2): 463–470.
APA
Chen, Cuiying, Schmilovtz-Weiss, H., Liu, X., Pappo, O., Halpern, M., Sulkes, J., Braun, M., et al. (2009). Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis. JOURNAL OF PROTEOME RESEARCH, 8(2), 463–470.
Vancouver
1.
Chen C, Schmilovtz-Weiss H, Liu X, Pappo O, Halpern M, Sulkes J, et al. Serum protein N-glycans profiling for the discovery of potential biomarkers for nonalcoholic steatohepatitis. JOURNAL OF PROTEOME RESEARCH. 2009;8(2):463–70.
MLA
Chen, Cuiying, Hemda Schmilovtz-Weiss, Xue-en Liu, et al. “Serum Protein N-glycans Profiling for the Discovery of Potential Biomarkers for Nonalcoholic Steatohepatitis.” JOURNAL OF PROTEOME RESEARCH 8.2 (2009): 463–470. Print.