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Inhibition of spontaneous neutrophil apoptosis by parabutoporin acts independently of NADPH oxidase inhibition but by lipid raft-dependent stimulation of Akt

(2009) JOURNAL OF LEUKOCYTE BIOLOGY. 85(3). p.497-507
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Abstract
Neutrophil cell death plays a crucial role in neutrophil homeostasis and the resolution of inflammation. The superoxide-producing NADPH oxidase is involved in pathogen degradation and subsequent activation of cell death programs. Neutrophils from patients with chronic granulomatous disease, who have a deficient NADPH oxidase activity, have been demonstrated previously to have a prolonged lifespan, suggesting that a basal NADPH oxidase activity also regulates spontaneous neutrophil turnover. The NADPH oxidase inhibitor parabutoporin (PP) does delay spontaneous apoptosis, but this effect is completely independent of NADPH oxidase inhibition. Instead, the prosurvival effect of PP depends on activation of protein kinase B/Akt via lipid raft signaling. Disruption of lipid rafts abrogates the prosurvival effect without interfering with NADPH oxidase activity. Furthermore, we cannot detect a different rate of spontaneous apoptosis between normal and NADPH oxidase-deficient neutrophils, arguing against a role of NADPH oxidase in spontaneous neutrophil apoptosis.
Keywords
chronic granulomatous disease, granulocyte, superoxide, scorpion, CHRONIC GRANULOMATOUS-DISEASE, NF-KAPPA-B, FAS LIGAND, IN-VITRO, ACTIVATION, INVOLVEMENT, MCL-1, MITOCHONDRIA, MECHANISMS, LEUKOCYTES

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Citation

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MLA
Remijsen, Quinten et al. “Inhibition of Spontaneous Neutrophil Apoptosis by Parabutoporin Acts Independently of NADPH Oxidase Inhibition but by Lipid Raft-dependent Stimulation of Akt.” JOURNAL OF LEUKOCYTE BIOLOGY 85.3 (2009): 497–507. Print.
APA
Remijsen, Q., Vanden Berghe, T., Parthoens, E., Asselbergh, B., Vandenabeele, P., & Willems, J. (2009). Inhibition of spontaneous neutrophil apoptosis by parabutoporin acts independently of NADPH oxidase inhibition but by lipid raft-dependent stimulation of Akt. JOURNAL OF LEUKOCYTE BIOLOGY, 85(3), 497–507.
Chicago author-date
Remijsen, Quinten, Tom Vanden Berghe, Eef Parthoens, Bob Asselbergh, Peter Vandenabeele, and Jean Willems. 2009. “Inhibition of Spontaneous Neutrophil Apoptosis by Parabutoporin Acts Independently of NADPH Oxidase Inhibition but by Lipid Raft-dependent Stimulation of Akt.” Journal of Leukocyte Biology 85 (3): 497–507.
Chicago author-date (all authors)
Remijsen, Quinten, Tom Vanden Berghe, Eef Parthoens, Bob Asselbergh, Peter Vandenabeele, and Jean Willems. 2009. “Inhibition of Spontaneous Neutrophil Apoptosis by Parabutoporin Acts Independently of NADPH Oxidase Inhibition but by Lipid Raft-dependent Stimulation of Akt.” Journal of Leukocyte Biology 85 (3): 497–507.
Vancouver
1.
Remijsen Q, Vanden Berghe T, Parthoens E, Asselbergh B, Vandenabeele P, Willems J. Inhibition of spontaneous neutrophil apoptosis by parabutoporin acts independently of NADPH oxidase inhibition but by lipid raft-dependent stimulation of Akt. JOURNAL OF LEUKOCYTE BIOLOGY. 2009;85(3):497–507.
IEEE
[1]
Q. Remijsen, T. Vanden Berghe, E. Parthoens, B. Asselbergh, P. Vandenabeele, and J. Willems, “Inhibition of spontaneous neutrophil apoptosis by parabutoporin acts independently of NADPH oxidase inhibition but by lipid raft-dependent stimulation of Akt,” JOURNAL OF LEUKOCYTE BIOLOGY, vol. 85, no. 3, pp. 497–507, 2009.
@article{595637,
  abstract     = {Neutrophil cell death plays a crucial role in neutrophil homeostasis and the resolution of inflammation. The superoxide-producing NADPH oxidase is involved in pathogen degradation and subsequent activation of cell death programs. Neutrophils from patients with chronic granulomatous disease, who have a deficient NADPH oxidase activity, have been demonstrated previously to have a prolonged lifespan, suggesting that a basal NADPH oxidase activity also regulates spontaneous neutrophil turnover. The NADPH oxidase inhibitor parabutoporin (PP) does delay spontaneous apoptosis, but this effect is completely independent of NADPH oxidase inhibition. Instead, the prosurvival effect of PP depends on activation of protein kinase B/Akt via lipid raft signaling. Disruption of lipid rafts abrogates the prosurvival effect without interfering with NADPH oxidase activity. Furthermore, we cannot detect a different rate of spontaneous apoptosis between normal and NADPH oxidase-deficient neutrophils, arguing against a role of NADPH oxidase in spontaneous neutrophil apoptosis.},
  author       = {Remijsen, Quinten and Vanden Berghe, Tom and Parthoens, Eef and Asselbergh, Bob and Vandenabeele, Peter and Willems, Jean},
  issn         = {0741-5400},
  journal      = {JOURNAL OF LEUKOCYTE BIOLOGY},
  keywords     = {chronic granulomatous disease,granulocyte,superoxide,scorpion,CHRONIC GRANULOMATOUS-DISEASE,NF-KAPPA-B,FAS LIGAND,IN-VITRO,ACTIVATION,INVOLVEMENT,MCL-1,MITOCHONDRIA,MECHANISMS,LEUKOCYTES},
  language     = {eng},
  number       = {3},
  pages        = {497--507},
  title        = {Inhibition of spontaneous neutrophil apoptosis by parabutoporin acts independently of NADPH oxidase inhibition but by lipid raft-dependent stimulation of Akt},
  url          = {http://dx.doi.org/10.1189/jlb.0908525},
  volume       = {85},
  year         = {2009},
}

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