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Non-contact optical tweezers-based single cell analysis through in vivo X-ray elemental imaging

Eva Vergucht (UGent) , Toon Brans (UGent) , Filip Beunis (UGent) , Jan Garrevoet (UGent) , Stephen Bauters (UGent) , Maarten De Rijcke (UGent) , David Deruytter (UGent) , Colin Janssen (UGent) , Christian Riekel, Manfred Burghammer (UGent) , et al.
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Abstract
We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in situ elemental imaging of free-standing living biological microorganisms or single cells in their aqueous environment. Using the model organism Scrippsiella trochoidea, several successful test experiments focussing on applications in environmental toxicology have been performed at ESRF-ID13, demonstrating the feasibility, repeatability and high throughput potential of the OT XRF methodology. We expect that the OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability.

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MLA
Vergucht, Eva, et al. “Non-Contact Optical Tweezers-Based Single Cell Analysis through in Vivo X-Ray Elemental Imaging.” Synchrotron and Neutron Workshop, Abstracts, 2015.
APA
Vergucht, E., Brans, T., Beunis, F., Garrevoet, J., Bauters, S., De Rijcke, M., … Vincze, L. (2015). Non-contact optical tweezers-based single cell analysis through in vivo X-ray elemental imaging. In Synchrotron and Neutron Workshop, Abstracts. Utrecht, The Netherlands.
Chicago author-date
Vergucht, Eva, Toon Brans, Filip Beunis, Jan Garrevoet, Stephen Bauters, Maarten De Rijcke, David Deruytter, et al. 2015. “Non-Contact Optical Tweezers-Based Single Cell Analysis through in Vivo X-Ray Elemental Imaging.” In Synchrotron and Neutron Workshop, Abstracts.
Chicago author-date (all authors)
Vergucht, Eva, Toon Brans, Filip Beunis, Jan Garrevoet, Stephen Bauters, Maarten De Rijcke, David Deruytter, Colin Janssen, Christian Riekel, Manfred Burghammer, and Laszlo Vincze. 2015. “Non-Contact Optical Tweezers-Based Single Cell Analysis through in Vivo X-Ray Elemental Imaging.” In Synchrotron and Neutron Workshop, Abstracts.
Vancouver
1.
Vergucht E, Brans T, Beunis F, Garrevoet J, Bauters S, De Rijcke M, et al. Non-contact optical tweezers-based single cell analysis through in vivo X-ray elemental imaging. In: Synchrotron and Neutron Workshop, Abstracts. 2015.
IEEE
[1]
E. Vergucht et al., “Non-contact optical tweezers-based single cell analysis through in vivo X-ray elemental imaging,” in Synchrotron and Neutron Workshop, Abstracts, Utrecht, The Netherlands, 2015.
@inproceedings{5956057,
  abstract     = {{We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in situ elemental imaging of free-standing living biological microorganisms or single cells in their aqueous environment. Using the model organism Scrippsiella trochoidea, several successful test experiments focussing on applications in environmental toxicology have been performed at ESRF-ID13, demonstrating the feasibility, repeatability and high throughput potential of the OT XRF methodology. 
We expect that the OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability.}},
  author       = {{Vergucht, Eva and Brans, Toon and Beunis, Filip and Garrevoet, Jan and Bauters, Stephen and De Rijcke, Maarten and Deruytter, David and Janssen, Colin and Riekel, Christian and Burghammer, Manfred and Vincze, Laszlo}},
  booktitle    = {{Synchrotron and Neutron Workshop, Abstracts}},
  language     = {{eng}},
  location     = {{Utrecht, The Netherlands}},
  title        = {{Non-contact optical tweezers-based single cell analysis through in vivo X-ray elemental imaging}},
  year         = {{2015}},
}